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Records with Keyword: Fermentation
Showing records 1 to 25 of 71. [First] Page: 1 2 3 Last
Study on Radio Frequency-Treated Agricultural Byproducts as Media for Hericium erinaceus Solid-State Fermentation for Whitening Effects
Zih-Yang Lin, Chia-Ling Yen, Su-Der Chen
June 6, 2024 (v1)
Keywords: agricultural byproducts, drying, Fermentation, radio frequency (RF), whitening
Hot air-assisted radio frequency (HARF) is considered a rapid heating process. In order to improve the circular economy of agricultural byproducts, this study used different proportions of HARF stabilized rice bran (R) from milling rice, HARF dried ginseng residue (G) from ultrasonic extraction, and peanut residue (P) from HARF roasting and oil extraction as the Hericium erinaceus solid-state fermented media. Then, the whitening effects of water extracts from media and fermented products were analyzed. First, the surface temperature of 1 kg rice bran exceeded 90 °C after 3 min of 5 kW HARF heating, effectively deactivating lipase. The combinations of 1 kg of rice bran with 0.5, 1, 1.5, and 2 kg of ginseng residue (85% moisture content) were dried using 5 kW HARF. Each of the drying rates was about 27 g/min, and the drying periods were 14, 30, 46, and 62 min, respectively, which were used to reduce the moisture content below 10%. Compared to traditional air drying for ginseng residue, H... [more]
Differential Analysis of Pomelo Peel Fermentation by Cordyceps militaris Based on Untargeted Metabolomics
Yannan Xiang, Siyi Tian, Xinyu Luo, Chenggang Cai, Yaowen Du, Hailong Yang, Haiyan Gao
June 5, 2024 (v1)
Keywords: Cordyceps militaris, Fermentation, multivariate statistical analysis, pomelo peel, untargeted metabolomics
The content of differentially abundant metabolites in the fermentation broth of grapefruit peels fermented by Cordyceps militaris at different fermentation times was analyzed via LC−MS/MS. Small molecule metabolites and differential metabolic pathways were analyzed via multivariate analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. A total of 423 metabolites were identified at 0, 2, 6, and 10 days after fermentation. Among them, 169 metabolites showed differential abundance, with significant differences observed between the fermentation liquids of every two experimental groups, and the metabolite composition in the fermentation liquid changed over the fermentation time. In summary, the upregulation and downregulation of metabolites in cancer metabolic pathways collectively promote the remodeling of cancer cell metabolism, facilitating increased glycolysis, alterations in TCA cycle flux, and enhanced biosynthesis of the macromolecules required for rapid proliferatio... [more]
Screening of Microbial Strains Used to Ferment Dendrobium officinale to Produce Polysaccharides, and Investigation of These Polysaccharides’ Skin Care Effects
Xin Tang, Bulei Wang, Bingyong Mao, Jianxin Zhao, Guangrong Liu, Kaiye Yang, Shumao Cui
November 30, 2023 (v1)
Subject: Biosystems
Keywords: Dendrobium officinale, Fermentation, Limosilactobacillus reuteri CCFM8631, polysaccharides
The microbial fermentation of plants is a promising approach for enhancing the yield of polysaccharides with increased activity. In this study, ten microbial strains, Lactiplantibacillus plantarum CCFM8661, Limosilactobacillus reuteri CCFM8631, Lactobacillus helveticus M10, Lacticaseibacillus rhamnosus CCFM237, Lactilactobacillus sakei GD17-9, Lacticaseibacillus casei CCFM1073, Bacillus subtilis CCFM1162, Bacteroides cellulosilyticus FTJSI-E-2, Bacteroides stercoris FNMHLBEIK-4, and Saccharomyces cerevisiae HN7-A5, were used to ferment Dendrobium officinale. The skin care activity of the resulting polysaccharides (F-DOP) was evaluated in cultured HaCaT and RAW 264.7 cells, and a mouse model. The results indicated that D. officinale medium promoted strain proliferation, and fermentation significantly enhanced polysaccharide yield (up to 1.42 g/L) compared to that without fermentation (0.76 g/L). Moreover, F-DOPs, especially after CCFM8631 fermentation, exhibited an excellent ability to... [more]
Waste-to-Energy Pipeline through Consolidated Fermentation−Microbial Fuel Cell (MFC) System
Kundan Kumar, Ling Ding, Haiyan Zhao, Ming-Hsun Cheng
September 21, 2023 (v1)
Keywords: agriculture residues (AR), bioenergy, Fermentation, microbial fuel cell (MFC), municipal solid waste (MSW)
The rise in population, urbanization, and industrial developments have led to a substantial increase in waste generation and energy demand, posing significant challenges for waste management as well as energy conservation and production. Bioenergy conversions have been merged as advanced, sustainable, and integrated solutions for these issues, encompassing energy generation and waste upcycling of different types of organic waste. Municipal solid waste (MSW) and agricultural residues (AR) are two main resources for bioenergy conversions. Bioenergy production involves feedstock deconstruction and the conversion of platform chemicals to energy products. This review provides a detailed overview of waste sources, biofuel, and bioelectricity production from fermentation and microbial fuel cell (MFC) technology, and their economic and environmental perspectives. Fermentation plays a critical role in liquid biofuel production, while MFCs demonstrate promising potential for simultaneous product... [more]
Fermentation of Menaquinone-7: The Influence of Environmental Factors and Storage Conditions on the Isomer Profile
Neha Lal, Mostafa Seifan, Aydin Berenjian
July 13, 2023 (v1)
Subject: Biosystems
Keywords: bioactivity, environmental factors, Fermentation, menaquinone-7 isomer profile, storage conditions
Menaquinone-7 (MK-7) provides significant health gains due to its excellent pharmacokinetic properties. However, MK-7 occurs at low concentrations in mainstream foods, heightening the demand for nutritional supplements. MK-7 exists as geometric isomers, and only all-trans MK-7 is bioactive. Exposure to certain environments impacts the isomer profile. Knowledge of these factors and their influence on the isomer composition is important, as the efficacy of fermented MK-7 end products is solely determined by the all-trans isomer. This investigation aimed to evaluate the short- and long-term effect of atmospheric oxygen, common temperatures, and light on the isomer profile. From the short-term study, it was ascertained that MK-7 is moderately heat-stable but extremely light-sensitive. The stability of all-trans MK-7 was then examined during 8 weeks of storage at a low temperature with minimal oxygen exposure in the absence of light. Negligible change in the all-trans MK-7 concentration occ... [more]
Application of Technological Processes to Create a Unitary Model for Energy Recovery from Municipal Waste
Robert Sidełko
April 20, 2023 (v1)
Keywords: biogas, combustion, energy recovery, Fermentation, model of energy recovery, waste management
The subject matter of this paper is the functioning of a highly effective waste management system. Assumptions of the Energy Recovery Waste Processing (ERWP) model, being a universal solution for towns and regions irrespective of their population, are presented here. The result of simulations illustrating the energetic potential of municipal waste stored and processed in biological and physicochemical processes are also presented. Calculations were performed for the municipality of Koszalin (Poland), with a population of 106,000. Mixed household and commercial waste, organic waste, waste from selective collection and sewage sludge from a municipal wastewater treatment plant were considered in the waste mass balance. Empirical equations and unit coefficients describing the energetic efficiency of particular processes originating from the author’s own research work as well as from the results available from the scientific literature were used in the calculations. The developed ERWP model... [more]
Experimental and Artificial Intelligence Modelling Study of Oil Palm Trunk Sap Fermentation
Leila Ezzatzadegan, Rubiyah Yusof, Noor Azian Morad, Parvaneh Shabanzadeh, Nur Syuhana Muda, Tohid N. Borhani
April 19, 2023 (v1)
Subject: Optimization
Keywords: ANFIS, Fermentation, neuro-fuzzy, oil palm trunk sap, particle swarm optimization (PSO)
Five major operations for the conversion of lignocellulosic biomasses into bioethanol are pre-treatment, detoxification, hydrolysis, fermentation, and distillation. The fermentation process is a significant biological step to transform lignocellulose into biofuel. The interactions of biochemical networks and their uncertainty and nonlinearity that occur during fermentation processes are major problems for experts developing accurate bioprocess models. In this study, mechanical processing and pre-treatment on the palm trunk were done before fermentation. Analysis was performed on the fresh palm sap and the fermented sap to determine the composition. The analysis for total sugar content was done using high-performance liquid chromatography (HPLC) and the percentage of alcohols by volume was determined using gas chromatography (GC). A model was also developed for the fermentation process based on the Adaptive-Network-Fuzzy Inference System (ANFIS) combined with particle swarm optimization... [more]
The Municipal Solid Waste Management System with Anaerobic Digestion
Przemysław Seruga
April 19, 2023 (v1)
Keywords: biogas, energy recovery, Fermentation, organic waste, soil amendment, waste to energy
This study investigated the applied methods for the collection and treatment of an organic fraction of municipal solid waste with anaerobic digestion (AD), including the effects of selective waste collection system introduction. As the research area, data from a waste treatment plant, which collects waste from about 260,000 inhabitants, was used as the selected waste management plan. Biowaste stream management was emphasized. Thus, research on energy recovery and the characteristics of digestate (nutrient and heavy metals content) obtained from biowaste AD was performed. The results of the studies and their quantitative data were interpreted. A significant discrepancy between the assumptions and the actual situation was revealed (up to 20% year-on-year regarding biowaste). An underestimation of the amount of waste when planning was noted. AD ensures energy recovery from biowaste, which can cover facility electricity needs and material recovery. The digestate might find agricultural usa... [more]
Biochemical Conversion of Lignocellulosic Biomass from Date Palm of Phoenix dactylifera L. into Ethanol Production
Yousra Antit, Inmaculada Olivares, Moktar Hamdi, Sebastián Sánchez
April 14, 2023 (v1)
Keywords: cellulosic fibers from date palm, enzymatic hydrolysis, Ethanol, Fermentation, P. tannophilus, pretreatment
Cellulosic fibers from date palm are among the most promising lignocellulose feedstock for biorefinery purposes. The world production is between 1.9 and 2.4 million t/year. Initially, a pretreatment with dilute-sulphuric acid of these fibers was performed using a response surface methodology, with temperature and process time as factors. The aim is to produce bioethanol from young and old fibers from date palm, Phoenix dactylifera L. Optimal thermochemical pretreatment conditions for both fibers palms were 220 °C in hydrothermal conditions (without acid); in these conditions pretreated young fibers presented a maximum content in holocelluloses of 45.18% and old fibers 61.97%. Subsequently, during the enzymatic hydrolysis a maximum yield of total reducing sugars (TRS) was reached, 46.32 g/100 g for pretreated dry young fibers and 48.54 g/100 g for pretreated dry old fibers. After enzymatic saccharification, hydrolysates were fermented by Pachysolen tannophilus (ATCC 32691) to ethanol, r... [more]
Hydrogen Dark Fermentation for Degradation of Solid and Liquid Food Waste
Vira Hovorukha, Olesia Havryliuk, Galina Gladka, Oleksandr Tashyrev, Antonina Kalinichenko, Monika Sporek, Agnieszka Dołhańczuk-Śródka
April 14, 2023 (v1)
Subject: Environment
Keywords: biohydrogen, environmental biotechnology, Fermentation, green energy, liquid food waste, solid food waste
The constant increase in the amount of food waste accumulating in landfills and discharged into the water reservoirs causes environment pollution and threatens human health. Solid and liquid food wastes include fruit, vegetable, and meat residues, alcohol bard, and sewage from various food enterprises. These products contain high concentrations of biodegradable organic compounds and represent an inexpensive and renewable substrate for the hydrogen fermentation. The goal of the work was to study the efficiency of hydrogen obtaining and decomposition of solid and liquid food waste via fermentation by granular microbial preparation (GMP). The application of GMP improved the efficiency of the dark fermentation of food waste. Hydrogen yields reached 102 L/kg of solid waste and 2.3 L/L of liquid waste. The fermentation resulted in the 91-fold reduction in the weight of the solid waste, while the concentration of organics in the liquid waste decreased 3-fold. Our results demonstrated the pote... [more]
Lactic Acid: A Comprehensive Review of Production to Purification
Abidemi Oluranti Ojo, Olga de Smidt
April 11, 2023 (v1)
Subject: Biosystems
Keywords: Fermentation, lactic acid, lignocellulose, microorganisms, pretreatments
Lactic acid (LA) has broad applications in the food, chemical, pharmaceutical, and cosmetics industries. LA production demand rises due to the increasing demand for polylactic acid since LA is a precursor for polylactic acid production. Fermentative LA production using renewable resources, such as lignocellulosic materials, reduces greenhouse gas emissions and offers a cheaper alternative feedstock than refined sugars. Suitable pretreatment methods must be selected to minimize LA cost production, as the successful hydrolysis of lignocellulose results in sugar-rich feedstocks for fermentation. This review broadly focused on fermentative LA production from lignocellulose. Aspects discussed include (i). low-cost materials for fermentative LA production, (ii). pretreatment methods, (iii). enzymatic hydrolysis of cellulose and hemicellulose, (iv). lactic acid-producing microorganisms, including fungi, bacteria, genetically modified microorganisms, and their fermentative pathways, and (v). f... [more]
Energy and Economic Analysis of Date Palm Biomass Feedstock for Biofuel Production in UAE: Pyrolysis, Gasification and Fermentation
Remston Martis, Amani Al-Othman, Muhammad Tawalbeh, Malek Alkasrawi
April 4, 2023 (v1)
Keywords: Aspen Plus, Biofuels, Biomass, energy integration, Fermentation, gasification, process simulation, pyrolysis
This work evaluates date palm waste as a cheap and available biomass feedstock in UAE for the production of biofuels. The thermochemical and biochemical routes including pyrolysis, gasification, and fermentation were investigated. Simulations were done to produce biofuels from biomass via Aspen Plus v.10. The simulation results showed that for a tonne of biomass feed, gasification produced 56 kg of hydrogen and fermentation yielded 233 kg of ethanol. Process energy requirements, however, proved to offset the bioethanol product value. For 1 tonne of biomass feed, the net duty for pyrolysis was 37 kJ, for gasification was 725 kJ, and for fermentation was 7481.5 kJ. Furthermore, for 1 tonne of date palm waste feed, pyrolysis generated a returned USD $768, gasification generated USD 166, but fermentation required an expenditure of USD 763, rendering it unfeasible. The fermentation economic analysis showed that reducing the system’s net duty to 6500 kJ/tonne biomass and converting 30% hemic... [more]
Oriented Fermentation of Food Waste towards High-Value Products: A Review
Qiao Wang, Huan Li, Kai Feng, Jianguo Liu
April 4, 2023 (v1)
Keywords: Ethanol, fatty acids, Fermentation, food waste, lactic acid, recovery
Food waste has a great potential for resource recovery due to its huge yield and high organic content. Oriented fermentation is a promising method with strong application prospects due to high efficiency, strong robustness, and high-value products. Different fermentation types lead to different products, which can be shifted by adjusting fermentation conditions such as inoculum, pH, oxidation-reduction potential (ORP), organic loading rate (OLR), and nutrients. Compared with other types, lactic acid fermentation has the lowest reliance on artificial intervention. Lactic acid and volatile fatty acids are the common products, and high yield and high purity are the main targets of food waste fermentation. In addition to operational parameters, reactors and processes should be paid more attention to for industrial application. Currently, continuously stirred tank reactors and one-stage processes are used principally for scale-up continuous fermentation of food waste. Electro-fermentation a... [more]
Life Cycle Analysis of the Bioethanol Production from Food Waste—A Review
Aikaterini Konti, Dimitris Kekos, Diomi Mamma
April 3, 2023 (v1)
Subject: Environment
Keywords: Biofuels, environmental impact, Fermentation, Renewable and Sustainable Energy, waste
Food Waste (FW) because of its composition is considered as an ideal feedstock for the production of biofuels and in particular bioethanol. The production of bioethanol from lignocellulosic materials has been studied over a long time. The process consists of the stages of pretreatment, enzymatic hydrolysis, fermentation and product recovery. However, the legal framework regarding biofuels has established specific environmental criteria for their production which are regularly updated. The most common tool for the assessment of the environmental performance of a process or product is the Life Cycle Analysis (LCA). In the present review, the results of LCA studies on the production of bioethanol from food waste are presented. Significant differences are observed among the studies in terms of the methodological choices made. Despite the high heterogeneity observed which does not allow a direct comparison among them, there is strong evidence that the production of bioethanol from food wast... [more]
Process Integration of Green Hydrogen: Decarbonization of Chemical Industries
Mohammad Ostadi, Kristofer Gunnar Paso, Sandra Rodriguez-Fabia, Lars Erik Øi, Flavio Manenti, Magne Hillestad
April 3, 2023 (v1)
Keywords: biogas upgrading, calcination, electrolysis, Fermentation, green hydrogen, iron reduction, municipal waste incineration, oxy-combustion, process integration, pulp production
Integrated water electrolysis is a core principle of new process configurations for decarbonized heavy industries. Water electrolysis generates H2 and O2 and involves an exchange of thermal energy. In this manuscript, we investigate specific traditional heavy industrial processes that have previously been performed in nitrogen-rich air environments. We show that the individual process streams may be holistically integrated to establish new decarbonized industrial processes. In new process configurations, CO2 capture is facilitated by avoiding inert gases in reactant streams. The primary energy required to drive electrolysis may be obtained from emerging renewable power sources (wind, solar, etc.) which have enjoyed substantial industrial development and cost reductions over the last decade. The new industrial designs uniquely harmonize the intermittency of renewable energy, allowing chemical energy storage. We show that fully integrated electrolysis promotes the viability of decarboniz... [more]
Thermophilic Co-Fermentation of Wood Wastes and High in Nitrogen Animal Manures into Bio-Methane with the Aid of Fungi and its Potential in the USA
John G. Ingersoll
March 31, 2023 (v1)
Subject: Biosystems
Keywords: archaea and fungi, bio-fertilizers, bio-methane, Carbon Dioxide, Fermentation, Hydrogen, modular industrial plant design, nitrogen-rich animal manures, thermophilic bacteria, water electrolysis, wind power, wood
A novel process is proposed whereby wood wastes from forest tree mortalities and improved forest management are co-digested with high in nitrogen content animal manures to yield bio-methane along with nitrogen, phosphorous, and potassium bio-fertilizers. The process mimics the well-known wood conversion to methane process in lower termites but relies on thermophilic fungi, bacteria, and archaea instead. It is based on the modified state-of-the art two-step, hyperthermophilic (70 °C) hydrolysis and thermophilic (55 °C) fermentation, dry (30% TS), anaerobic digestion technology with a high organic loading and shortened retention time. The process is augmented with the thermophilic fermentation of carbon dioxide in the biogas into secondary bio-methane by employing hydrogen produced via wind-powered electrolysis. The entire process comprised of five distinct steps is designated as “Wood to Methane 3 + 2”. An industrial type, standardized plant unit has been developed that can be employed... [more]
Fermentation of Ferulated Arabinoxylan Recovered from the Maize Bioethanol Industry
Mayra A. Mendez-Encinas, Dora E. Valencia-Rivera, Elizabeth Carvajal-Millan, Humberto Astiazaran-Garcia, Valérie Micard, Agustín Rascón-Chu
March 28, 2023 (v1)
Subject: Biosystems
Keywords: antiproliferative agent, colon cancer, Fermentation, ferulated arabinoxylan, ferulic acid
Maize by-product from the bioethanol industry (distiller’s dried grains with solubles, DDGS) is a source of ferulated arabinoxylan (AX), which is a health-promoting polysaccharide. In the present study, AX from DDGS was fermented by a representative colonic bacterial mixture (Bifidobacterium longum, Bifidobacterium adolescentis, and Bacteroides ovatus), and the effect of the fermented AX (AX-f) on the proliferation of the cell line Caco-2 was investigated. AX was efficiently metabolized by these bacteria, as evidenced by a decrease in the polysaccharide molecular weight from 209 kDa to < 50 kDa in AX-f, the release of ferulic acid (FA) from polysaccharide chains (1.14 µg/mg AX-f), and the short-chain fatty acids (SCFA) production (277 µmol/50 mg AX). AX-f inhibited the proliferation of Caco-2 cells by 80−40% using concentrations from 125−1000 µg/mL. This dose-dependent inverse effect was attributed to the increased viscosity of the media due to the polysaccharide concentration. The... [more]
An Overview of the Factors Influencing Apple Cider Sensory and Microbial Quality from Raw Materials to Emerging Processing Technologies
Paul Cristian Calugar, Teodora Emilia Coldea, Liana Claudia Salanță, Carmen Rodica Pop, Antonella Pasqualone, Cristina Burja-Udrea, Haifeng Zhao, Elena Mudura
March 28, 2023 (v1)
Subject: Materials
Keywords: apple cider, emerging technologies, Fermentation, sensory profile, volatile compounds
Given apple, an easily adapted culture, and a large number of apple varieties, the production of apple cider is widespread globally. Through the fermentation process, a series of chemical changes take place depending on the apple juice composition, type of microorganism involved and technology applied. Following both fermentations, alcoholic and malo-lactic, and during maturation, the sensory profile of cider changes. This review summarises the current knowledge about the influence of apple variety and microorganisms involved in cider fermentation on the sensory and volatile profiles of cider. Implications of both Saccharomyces, non-Saccharomyces yeast and lactic acid bacteria, respectively, are discussed. Also are presented the emerging technologies applied to cider processing (pulsed electric field, microwave extraction, enzymatic, ultraviolet and ultrasound treatments, high-pressure and pulsed light processing) and the latest trends for a balanced production in terms of sustainabili... [more]
Statistical Optimization of Alkali Pretreatment to Improve Sugars Recovery from Spent Coffee Grounds and Utilization in Lactic Acid Fermentation
Kang Hyun Lee, Ye Won Jang, Jeongho Lee, Seunghee Kim, Chulhwan Park, Hah Young Yoo
March 28, 2023 (v1)
Keywords: alkali pretreatment, enzymatic hydrolysis, Fermentation, lactic acid, Optimization, spent coffee grounds
Biorefinery, which utilizes carbon-neutral biomass as a resource, is attracting attention as a significant alternative in a modern society confronted with climate change. In this study, spent coffee grounds (SCGs) were used as the feedstock for lactic acid fermentation. In order to improve sugar conversion, alkali pretreatment was optimized by a statistical method, namely response surface methodology (RSM). The optimum conditions for the alkali pretreatment of SCGs were determined as follows: 75 °C, 3% potassium hydroxide (KOH) and a time of 2.8 h. The optimum conditions for enzymatic hydrolysis of pretreated SCGs were determined as follows: enzyme complex loading of 30-unit cellulase, 15-unit cellobiase and 50-unit mannanase per g biomass and a reaction time of 96 h. SCG hydrolysates were used as the carbon source for Lactobacillus cultivation, and the conversions of lactic acid by L. brevis ATCC 8287 and L. parabuchneri ATCC 49374 were 40.1% and 55.8%, respectively. Finally, the maxi... [more]
Biowaste Treatment and Waste-To-Energy—Environmental Benefits
Martin Pavlas, Jan Dvořáček, Thorsten Pitschke, René Peche
March 24, 2023 (v1)
Subject: Environment
Keywords: biowaste, composting, Fermentation, global warming potential, greenhouse gases, waste-to-energy
Biowaste represents a significant fraction of municipal solid waste (MSW). Its separate collection is considered as a useful measure to enhance waste management systems in both the developed and developing world. This paper aims to compare the environmental performance of three market-ready technologies currently used to treat biowaste—biowaste composting, fermentation, and biowaste incineration in waste-to-energy (WtE) plants as a component of residual municipal solid waste (RES). Global warming potential (GWP) was applied as an indicator and burdens related to the operation of facilities and credits obtained through the products were identified. The environmental performance of a WtE plant was investigated in detail using a model, implementing an approach similar to marginal-cost and revenues, which is a concept widely applied in economics. The results show that all of the treatment options offer an environmentally friendly treatment (their net GWP is negative). The environmental per... [more]
Production of Itaconic Acid from Cellulose Pulp: Feedstock Feasibility and Process Strategies for an Efficient Microbial Performance
Abraham A. J. Kerssemakers, Pablo Doménech, Marco Cassano, Celina K. Yamakawa, Giuliano Dragone, Solange I. Mussatto
March 24, 2023 (v1)
Subject: Biosystems
Keywords: Aspergillus terreus, biorefinery, C:N ratio, cellulose pulp, Fermentation, hydrolysis, itaconic acid, lignocellulosic biomass, oxygen availability
This study assessed the feasibility of using bleached cellulose pulp from Eucalyptus wood as a feedstock for the production of itaconic acid by fermentation. Additionally, different process strategies were tested with the aim of selecting suitable conditions for an efficient production of itaconic acid by the fungus Aspergillus terreus. The feasibility of using cellulose pulp was demonstrated through assays that revealed the preference of the strain in using glucose as carbon source instead of xylose, mannose, sucrose or glycerol. Additionally, the cellulose pulp was easily digested by enzymes without requiring a previous step of pretreatment, producing a glucose-rich hydrolysate with a very low level of inhibitor compounds, suitable for use as a fermentation medium. Fermentation assays revealed that the technique used for sterilization of the hydrolysate (membrane filtration or autoclaving) had an important effect in its composition, especially on the nitrogen content, consequently af... [more]
Assessment of the Effect of Nitrogen Concentration on Fermentation and Selection of a Highly Competitive Saccharomyces cerevisiae Strain for Efficient Ethanol Production
Patricia Portero Barahona, Jesús Martín-Gil, Pablo Martín-Ramos, Ana Briones Pérez, Enrique Javier Carvajal Barriga
March 22, 2023 (v1)
Keywords: Ethanol, Fermentation, nitrogen, Saccharomyces cerevisiae
The optimum nitrogen concentration for media supplementation and strain dominance are aspects of key importance to the industrial production of ethanol with a view to reducing costs and increasing yields. In this work, these two factors were investigated for four ethanologenic Saccharomyces cerevisiae strains (CLQCA-INT-001, CLQCA-INT-005, CLQCA-10-099, and UCLM 325), selected from the screening of 150 isolates, mostly from Ecuadorian yeast biodiversity. The effect of nitrogen concentration was assessed in terms of cellular growth, glucose consumption and ethanol production, and the yeast strains’ dominance was evaluated in continuous co-fermentation with cellular recycling by mitochondrial DNA analyses. Among the four selected yeast strains under study, CLQCA-INT-005 presented the highest glucose consumption at a nitrogen supplement concentration as low as 0.4 g·L−1, attaining an ethanol yield of up to 96.72% in 24 h. The same yeast strain was found to be highly competitive, showing a... [more]
Application of Industrial Wastewater and Sewage Sludge for Biohydrogen Production
Monika Šabić Runjavec, Marija Vuković Domanovac, Ante Jukić
March 17, 2023 (v1)
Subject: Biosystems
Keywords: biohydrogen, Fermentation, hydrogen production potential, industrial wastewater, low-cost substrate, microorganisms, waste activated sludge, waste stream
Recent technological developments have led to a significant increase in energy consumption in daily life. The search for alternative means of energy production has become an important task for applied sciences and modern technology. Hydrogen technology has great potential as a source of clean energy. The production of green hydrogen is a desirable and beneficial way to contribute to the decarbonization of the energy sector. In response to the demand for environmentally friendly and economically feasible approaches, biohydrogen production from waste materials has recently attracted interest. Waste materials from industrial or municipal production can be used as low-cost substrates for biohydrogen production through microbial degradation. Green energy needs could be met through a form of sustainable development that moves hand in hand with the harnessing of the microbial potential of waste biomass. Reuse of waste materials leads to pollution reductions and energy recycling. The aim of th... [more]
Enzymatic Co-Fermentation of Onion Waste for Bioethanol Production Using Saccharomyces cerevisiae and Pichia pastoris
Iqra Shahid, Ghulam Hussain, Mehwish Anis, Muhammad Umar Farooq, Muhammad Usman, Yasser Fouad, Jaroslaw Krzywanski
March 17, 2023 (v1)
Subject: Materials
Keywords: bioethanol, co-culture, Fermentation, Pichia pastoris, Saccharomyces cerevisiae, waste-to-energy
This paper evaluates the feasibility of bioethanol production from onion waste by Saccharomyces cerevisiae and Pichia pastoris and their novel co-culture through fermentation. The process parameters were optimized for each strain and their combination to observe the synergistic effect of co-fermentation. A dinitro salicylic acid (DNS) test was conducted to study the reducing sugar content of samples at different time intervals. Fourier transform infrared (FTIR) spectroscopic analysis was used to compare results for functional groups of samples before and after fermentation, and gas chromatography with flame ionization detection (GC-FID) analysis was performed to measure the bioethanol concentration obtained at different combinations of pH (5, 5.5, 6), temperature (20 °C, 30 °C, 40 °C), and time (24−110 h). The maximum bioethanol concentration was achieved through a monoculture of Saccharomyces cerevisiae, i.e., 30.56 g/L. The ethanol productivity was determined based on the ethanol con... [more]
Microalgal Hydrogen Production in Relation to Other Biomass-Based Technologies—A Review
Marcin Dębowski, Magda Dudek, Marcin Zieliński, Anna Nowicka, Joanna Kazimierowicz
March 9, 2023 (v1)
Keywords: Biofuels, Fermentation, Hydrogen, microalgae, thermochemical methods
Hydrogen is an environmentally friendly biofuel which, if widely used, could reduce atmospheric carbon dioxide emissions. The main barrier to the widespread use of hydrogen for power generation is the lack of technologically feasible and—more importantly—cost-effective methods of production and storage. So far, hydrogen has been produced using thermochemical methods (such as gasification, pyrolysis or water electrolysis) and biological methods (most of which involve anaerobic digestion and photofermentation), with conventional fuels, waste or dedicated crop biomass used as a feedstock. Microalgae possess very high photosynthetic efficiency, can rapidly build biomass, and possess other beneficial properties, which is why they are considered to be one of the strongest contenders among biohydrogen production technologies. This review gives an account of present knowledge on microalgal hydrogen production and compares it with the other available biofuel production technologies.
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