Work on synthetic biology has largely used a component-based metaphor for system construction. While this paradigm has been successful for the construction of numerous systems, the incorporation of contextual design issues—either compositional, host or environmental—will be key to realising more complex applications. Here, we present a design framework that radically steps away from a purely parts-based paradigm by using aspect-oriented software engineering concepts. We believe that the notion of concerns is a powerful and biologically credible way of thinking about system synthesis. By adopting this approach, we can separate core concerns, which represent modular aims of the design, from cross-cutting concerns, which represent system-wide attributes. The explicit handling of cross-cutting concerns allows for contextual information to enter the design process in a modular way. As a proof-of-principle, we implemented the aspect-oriented approach in the Python tool, SynBioWeaver, which e... [more]

Over the last several years, the trend of researchers has been to use some very low-cost materials as adsorbents. For this purpose, some already commercially used bast fibers were selected as potential adsorbent materials to remove basic dye from synthetic effluents. The adsorption of basic yellow 37 dye was studied using three different bast fibers under the names of flax, ramie, and kenaf. Their morphological structure was examined using several techniques such as scanning electron microscopy (SEM), crystallinity, X-Ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), as well as those characterizations being a useful tool to propose a mechanism of the whole adsorption process. The adsorption evaluation was achieved by studying at first the pH (12) and temperature effects (25⁻55 °C). Two isotherm models (Langmuir and Freundlich) were also applied to the experimental equilibrium data revealing the superiority of ramie fibers (327, 435, and 460 mg·g−1 (25 °C) for ke... [more]

In recent years, membrane technologies have been developed to address water shortage and energy crisis. Forward osmosis (FO), as an emerging membrane-based water treatment technology, employs an extremely concentrated draw solution (DS) to draw water pass through the semi-permeable membrane from a feed solution. DS as a critical material in FO process plays a key role in determining separation performance and energy cost. Most of existing DSs after FO still require a regeneration step making its return to initial state. Therefore, selecting suitable DS with low reverse solute, high flux, and easy regeneration is critical for improving FO energy efficiency. Numerous novel DSs with improved performance and lower regeneration cost have been developed. However, none reviews reported the categories of DS based on the energy used for recovery up to now, leading to the lack of enough awareness of energy consumption in DS regeneration. This review will give a comprehensive overview on the exis... [more]

In this contribution, we aim to show that opinion dynamics and population balance modeling can benefit from an exchange of problems and methods. To support this claim, the Deffuant-Weisbuch model, a classical approach in opinion dynamics, is formulated as a population balance model. This new formulation is subsequently analyzed in terms of moment equations, and conservation of the first and second order moment is shown. Exemplary results obtained by our formulation are presented and agreement with the original model is found. In addition, the influence of the initial distribution is studied. Subsequently, the Deffuant-Weisbuch model is transferred to engineering and interpreted as mass transfer between liquid droplets which results in a more flexible formulation compared to alternatives from the literature. On the one hand, it is concluded that the transfer of opinion-dynamics problems to the domain of population balance modeling offers some interesting insights as well as stimulating... [more]

Solar energy is becoming a promising source of heat and power for electrical generation and desalination plants. In this work, an integrated study of modeling, optimization, and experimental work is undertaken for a parabolic trough concentrator combined with a humidification and dehumidification desalination unit. The objective is to study the design performance and economic feasibility of a solar-driven desalination system. The design involves the circulation of a closed loop of synthetic blend motor oil in the concentrators and the desalination unit heat input section. The air circulation in the humidification and dehumidification unit operates in a closed loop, where the circulating water runs during the daytime and requires only makeup feed water to maintain the humidifier water level. Energy losses are reduced by minimizing the waste of treated streams. The process is environmentally friendly, since no significant chemical treatment is required. Design, construction, and operatio... [more]

The present work gives a critical overview of the recent progresses and new perspectives in the field of photocatalytic membranes (PMs) in photocatalytic membrane reactors (PMRs), thus highlighting the main advantages and the still existing limitations for large scale applications in the perspective of a sustainable growth. The classification of the PMRs is mainly based on the location of the photocatalyst with respect to the membranes and distinguished in: (i) PMRs with photocatalyst solubilized or suspended in solution and (ii) PMRs with photocatalyst immobilized in/on a membrane (i.e., a PM). The main factors affecting the two types of PMRs are deeply discussed. A multidisciplinary approach for the progress of research in PMs and PMRs is presented starting from selected case studies. A special attention is dedicated to PMRs employing dispersed TiO₂ confined in the reactor by a membrane for wastewater treatment. Moreover, the design and development of efficient photocatalytic membran... [more]

The article summarizes a systematic process design for the extraction and purification of artemisinin from annual mugwort (Artemisia annua L.). Artemisinin serves as an anti-malaria drug, therefore, resource-efficient and economic processes for its production are needed. The process design was based on lab-scale experiments and afterwards piloted on miniplant-scale at the institute. In this part of the article, a detailed economic feasibility studies including a reference process as a benchmark the lab-scale process and the pilot-scale process is given. Relevant differences between the different scales are discussed. The details of the respective unit operations (solid-liquid extraction, liquid-liquid extraction, chromatography and crystallization) are presented in dedicated articles. The study showed that even miniaturized lab-scale experiments are able to deliver data detailed enough for scale-up calculations on a theoretical basis. To our knowledge, a comparable systematic process d... [more]

In recent papers we have discussed the use of cuboid packed-bed devices as alternative to columns for chromatographic separations. These devices address some of the major flow distribution challenges faced by preparative columns used for process-scale purification of biologicals. Our previous studies showed that significant improvements in separation metrics such as the number of theoretical plates, peak shape, and peak resolution in multi-protein separation could be achieved. However, the length-to-width aspect ratio of a cuboid packed-bed device could potentially affect its performance. A systematic comparison of six cuboid packed-bed devices having different length-to-width aspect ratios showed that it had a significant effect on separation performance. The number of theoretical plates per meter in the best-performing cuboid packed-bed device was about 4.5 times higher than that in its equivalent commercial column. On the other hand, the corresponding number in the worst-performing... [more]

Groundwater can cause many hazardous problems when a tunnel is excavating. Seepage force acting on the support structure and the tunnel surface cannot be negligible. Under high groundwater table condition, the seepage situation becomes more complex and it is more difficult to control the leakage of groundwater to flow into a tunnel. In the paper, a multiple times grouting method is proposed, and the mechanical deformation behavior of surrounding rock is analyzed using the FLAC3D (Fast Lagrangian Analysis of Continua in 3 Dimensions) software according to the high groundwater table condition of the Hokusatsu tunnel. The results present that multiple times grouting can control leakage and the rock deformation well, compared with one-time grouting condition in rock breaking and high water pressure area. The seepage force decrease around the tunnel and the displacement is controlled effectively. The pore pressure reduces inside the grouting zone using a new kind of grouting material, which... [more]

The regeneration of red blood cells (RBCs) after blood loss is an individual complex process. We present a novel simple compartment model which is able to capture the most important features and can be personalized using parameter estimation. We compare predictions of the proposed and personalized model to a more sophisticated state-of-the-art model for erythropoiesis, and to clinical data from healthy subjects. We discuss the choice of model parameters with respect to identifiability. We give an outlook on how extensions of this novel mathematical model could have an important impact for personalized clinical decision support in the case of polycythemia vera (PV). PV is a slow-growing type of blood cancer, where especially the production of RBCs is increased. The principal treatment targeting the symptoms of PV is bloodletting (phlebotomy), at regular intervals that are based on personal experiences of the physicians. Model-based decision support might help to identify optimal and ind... [more]

In this work, the influence of the Hurst exponent and Peclet number (Pe) on the temporal mixing behavior of a conservative solute in the self-affine fractures with variable-aperture fracture and constant-aperture distributions were investigated. The mixing was quantified by the scalar dissipation rate (SDR) in fractures. The investigation shows that the variable-aperture distribution leads to local fluctuation of the temporal evolution of the SDR, whereas the temporal evolution of the SDR in the constant-aperture fractures is smoothly decreasing as a power-law function of time. The Peclet number plays a dominant role in the temporal evolution of mixing in both variable-aperture and constant-aperture fractures. In the constant-aperture fracture, the influence of Hurst exponent on the temporal evolution of the SDR becomes negligible when the Peclet number is relatively small. The longitudinal SDR can be related to the global SDR in the constant-aperture fracture when the Peclet number is... [more]

The Effectiveness-Number of Transfer Unit method (ε-NTU method) was applied to determine the overall mass transfer coefficient, KLa, of operating gas-liquid absorbers treating Volatile Organic Compounds (VOCs). This method requires the knowledge of the operating conditions (gas flow rate, QG; liquid flow rate, QL; scrubber volume V), the measurement of gaseous concentrations at the inlet, CGin, and at the outlet, CGout, of the contactor (in order to determine the effectiveness of the absorber ε) and the calculation of the Henry coefficient of the VOC between the gas and the liquid phases (HVOC). Coupled with the “equivalent absorption capacity„ concept, the ε-NTU method was used to determine KLa of absorbers contacting a gas and a mixture of water and a Non Aqueous Phase, successfully. The method, validated from literature data for configurations countercurrent scrubbers and stirred tank reactors, could be used to simply determine the overall mass transfer coefficient of systems for wh... [more]

The adsorptive properties of poly(1-methylpyrrol-2-ylsquaraine) (PMPS) particles were investigated in batch and column adsorption experiments as alternative adsorbent for the treatment of endocrine-disrupting chemicals in water. The PMPS particles were synthesised by condensing 3,4-dihydroxycyclobut-3-ene-1,2-dione (squaric acid) with 1-methylpyrrole in butanol. The results demonstrated that PMPS particles are effective in the removal of endocrine disrupting chemicals (EDCs) in water with adsorption being more favourable at an acidic pH, and a superior sorption capacity being achieved at pH 4. The results also showed that the removal of EDCs by the PMPS particles was a complex process involving multiple rate-limiting steps and physicochemical interactions between the EDCs and the particles. Gibbs free energy of −8.32 kJ/mole and −6.6 kJ/mol, and enthalpies of 68 kJ/mol and 43 kJ/mol, were achieved for the adsorption E2 and EE2 respectively The removal efficiencies of the EDCs by PMPS p... [more]

An experimental study in industry was previously carried out on a batch fluid bed granulation system by varying the inlet fluidizing air temperature, binder liquid spray atomization pressure, the binder liquid spray rate and the disintegrant composition in the formulation. A population balance model framework integrated with heat transfer and moisture balance due to liquid addition and evaporation was developed to simulate the fluid bed granulation system. The model predictions were compared with the industry data, namely, the particle size distributions (PSDs) and geometric mean diameters (GMDs) at various time-points in the granulation process. The model also predicted the trends for binder particle dissolution in the wetting liquid and the temperatures of the bed particles in the fluid bed granulator. Lastly, various process parameters were varied and extended beyond the region studied in the aforementioned experimental study to identify optimal regimes for granulation.

Supercritical carbon dioxide (S-CO₂) Brayton cycles (BC) are soon to be a competitive and environment friendly power generation technology. Progressive technological developments in turbo-machineries and heat exchangers have boosted the idea of using S-CO₂ in a closed-loop BC. This paper describes and discusses energy and exergy analysis of S-CO₂ BC in cascade arrangement with a secondary cycle using CO₂, R134a, ammonia, or argon as working fluids. Pressure drop in the cycle is considered, and its effect on the overall performance is investigated. No specific heat source is considered, thus any heat source capable of providing temperature in the range from 500 °C to 850 °C can be utilized, such as solar energy, gas turbine exhaust, nuclear waste heat, etc. The commercial software ‘Aspen HYSYS version 9’ (Aspen Technology, Inc., Bedford, MA, USA) is used for simulations. Comparisons with the literature and simulation results are discussed first for the standalone S-CO₂ BC. Energy analys... [more]

The coupling between hydraulic and mechanical processes in rock joints has significantly influenced the properties and applications of rock mass in many engineering fields. In this study, a series of regular shear tests and shear-flow coupled tests were conducted on artificial joints with sawtooth asperities. Shear deformation, strength, and seepage properties were comprehensively analyzed to reveal the influence of joint roughness, normal stress, and seepage pressure on shear-flow coupled behavior. The results indicate that the shear failure mode, which can be divided into sliding and cutting, is dominated by joint roughness and affected by the other two factors under certain conditions. The seepage process makes a negative impact on shear strength as a result of the mutual reinforcing of offsetting and softening effects. The evolution of hydraulic aperture during the shear-flow coupled tests embodies a consistent pattern of four stages: shear contraction, shear dilation, re-contracti... [more]

The degradation and detachment of the polyamide (PA) layer for the conventional thin-film composite (TFC) membranes due to chemical disinfectants cleaning with chlorine and material difference of PA layer and substrate are two major bottlenecks of forward osmosis (FO) technology. In this study, a new type of FO membranes was first prepared by controlling dopamine (DA) as the sole amine in the aqueous phase and the reaction with trimesoyl chloride (TMC) as the acyl chloride during interfacial polymerization (IP) process. The influence of membrane synthesis parameters such as monomer concentration, pH of the aqueous phase, IP reaction time and IP temperature were systematically investigated. The optimized membrane showed both improved structure stability and chlorine resistance, more so than the conventional TFC membrane. In general, novel DA/TMC TFC membranes could be an effective strategy to synthesize high-performance FO membranes with excellent structural stability and chlorine resis... [more]

When the hard and thick key strata are located above the working face, the bed separation structure is easy to be formed after mining because of the high strength and integrity of the hard and thick key strata and the initial breaking step is large. After the hard, thick strata are broken, the overburden will be largely collapsed and unstable in a large area and the dynamic disaster is easily induced. In this study, considering the fundamental deformation and failure effect of coal seam, the development law of the bed separation and the fractures under hard and thick magmatic rocks and the mechanism of breaking induced disaster of hard and thick magmatic rocks are studied by similar simulation tests. The results of the study are as follows: (1) The similar material ratio of coal seam is obtained by low-strength orthogonal ratio test of similar materials of coal seam, that is, cement:sand:water:activated carbon:coal = 6:6:7:1.1:79.9. (2) The magmatic rocks play a role in shielding the d... [more]

The existence of surface roughness, various contact conditions and the occurrence of flow nonlinearity make the flow process in natural rock fractures more complicated. To evaluate the fluid flow regimes in deformable rough-walled rock fractures, a great many hydromechanical tests were conducted on nine real fractures artificially produced from a wide range of lithological diversity. For fractures with a certain JRC (fracture roughness coefficient) value, the confining pressure varied from 5 to 20 MPa, and the hydraulic pressure was increased from 0.4 to 6.0 MPa. The experimental results display that (i) regression analyses of the raw experimental data indicate that the Forchheimer’s law provides a perfect description for flow process through the fractures. The coefficients of viscous and inertial pressure drops undergo a growth of 2⁻3 orders of magnitude with an increase in the confining pressure; (ii) the hydraulic aperture decreases by approximately 87.41⁻92.81% as the confining pre... [more]

Synthetic biology seeks to design biological parts and circuits that implement new functions in cells. Major accomplishments have been reported in this field, yet predicting a priori the in vivo behaviour of synthetic gene circuits is major a challenge. Mathematical models offer a means to address this bottleneck. However, in biology, modelling is perceived as an expensive, time-consuming task. Indeed, the quality of predictions depends on the accuracy of parameters, which are traditionally inferred from poorly informative data. How much can parameter accuracy be improved by using model-based optimal experimental design (MBOED)? To tackle this question, we considered an inducible promoter in the yeast S. cerevisiae. Using in vivo data, we re-fit a dynamic model for this component and then compared the performance of standard (e.g., step inputs) and optimally designed experiments for parameter inference. We found that MBOED improves the quality of model calibration by ∼60%. Results furt... [more]

Pulsed field gradient (PFG) nuclear magnetic resonance (NMR) allows recording of molecular diffusion paths (notably, the probability distribution of molecular displacements over typically micrometers, covered during an observation time of typically milliseconds) and has thus proven to serve as a most versatile means for the in-depth study of mass transfer in complex materials. This is particularly true with nanoporous host materials, where PFG NMR enabled the first direct measurement of intracrystalline diffusivities of guest molecules. Spatial resolution, i.e., the minimum diffusion path length experimentally observable, is limited by the time interval over which the pulsed field gradients may be applied. In “conventional„ PFG NMR measurements, this time interval is determined by a characteristic quantity of the host-guest system under study, the so-called transverse nuclear magnetic relaxation time. This leads, notably when considering systems with low molecular mobilities, to severe... [more]

Coal and gas outbursts are serious safety concerns in the Chinese coal industry. Mining of the upper or lower protective coal seams has been widely used to minimize this problem. This paper presents new findings from longwall mining-induced fractures, stress distribution changes in roof strata, strata movement and gas flow dynamics after the lower protective coal seam is extracted in a deep underground coal mine in Jincheng, China. Two Flac3D models with varying gob loading characteristics as a function of face advance were analyzed to assess the effect of gob behavior on stress relief in the protected coal seam. The gob behavior in the models is incorporated by applying variable force to the floor and roof behind the longwall face to simulate gob loading characteristics in the field. The influence of mining height on the stress-relief in protected coal seam is also incorporated. The stress relief coefficient and relief angle were introduced as two essential parameters to evaluate the... [more]

Since modeling and simulation are the two most effective tools that can be used in the design or analysis process, they play a vital role in developing such system. In many cases, they are the only possible means of making a safe engineering decision for a new concept of process for a large-scale system. Elsewhere, they are used as a critical element in the analysis of energy systems or to suggest a method of developing a novel and effective energy system model. Thus, in this study, simulations and test bed experiment were carried out to assess a low-power digital excitation system in order to validate its effectiveness. The excitation systems currently used by most of the power stations in the Republic of Korea were installed during the 1970s or 1980s. Unfortunately, it is difficult to seek technical assistance for them as they depend on foreign technologies, requiring a large sum to be paid when requesting one or more engineers to be dispatched. As such, technical updates have always... [more]

The fluid dynamics of macroscopic and microscopic systems is well developed and has been extensively validated. Its extraordinary success makes it tempting to apply Navier⁻Stokes fluid dynamics without modification to systems of ever decreasing dimensions as studies of nanofluidics become more prevalent. However, this can result in serious error. In this paper, we discuss several ways in which nanoconfined fluid flow differs from macroscopic flow. We give particular attention to several topics that have recently received attention in the literature: slip, spin angular momentum coupling, nonlocal stress response and density inhomogeneity. In principle, all of these effects can now be accurately modelled using validated theories. Although the basic principles are now fairly well understood, much work remains to be done in their application.

Lignin valorization is a key aspect to design sustainable management systems for lignocellulosic biomass. The successful implementation of bio-refineries requires high value added applications for the chemicals derived from lignin. Without effective separation processes, the achievement of this purpose is difficult. Supported ionic liquid membranes can play a relevant role in the separation and purification of lignocellulosic components. This work investigated different supported ionic liquid membranes for selective transport of two different types of technical lignins (Kraft lignin and lignosulphonate) and monosaccharides (xylose and glucose) in aqueous solution. Although five different membrane supports and nine ionic liquids were tested, only the system composed by [BMIM][DBP] as an ionic liquid and polytetrafluoroethylene (PTFE) as a membrane support allowed the selective transport of the tested solutes. The results obtained with this selective membrane demonstrated that lignins we... [more]