LAPSE:2019.0470
Published Article
LAPSE:2019.0470
Effect of the Length-to-Width Aspect Ratio of a Cuboid Packed-Bed Device on Efficiency of Chromatographic Separation
Guoqiang Chen, Raja Ghosh
April 8, 2019
In recent papers we have discussed the use of cuboid packed-bed devices as alternative to columns for chromatographic separations. These devices address some of the major flow distribution challenges faced by preparative columns used for process-scale purification of biologicals. Our previous studies showed that significant improvements in separation metrics such as the number of theoretical plates, peak shape, and peak resolution in multi-protein separation could be achieved. However, the length-to-width aspect ratio of a cuboid packed-bed device could potentially affect its performance. A systematic comparison of six cuboid packed-bed devices having different length-to-width aspect ratios showed that it had a significant effect on separation performance. The number of theoretical plates per meter in the best-performing cuboid packed-bed device was about 4.5 times higher than that in its equivalent commercial column. On the other hand, the corresponding number in the worst-performing cuboid-packed bed was lower than that in the column. A head-to-head comparison of the best-performing cuboid packed bed and its equivalent column was carried out. Performance metrics compared included the widths and dispersion indices of flow-through and eluted protein peaks. The optimized cuboid packed-bed device significantly outperformed its equivalent column with regards to all these attributes.
Keywords
bioseparation, chromatography, chromatography box, cuboid packed-bed, protein, separation efficiency
Suggested Citation
Chen G, Ghosh R. Effect of the Length-to-Width Aspect Ratio of a Cuboid Packed-Bed Device on Efficiency of Chromatographic Separation. (2019). LAPSE:2019.0470
Author Affiliations
Chen G: Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L7, Canada
Ghosh R: Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L7, Canada
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Journal Name
Processes
Volume
6
Issue
9
Article Number
E160
Year
2018
Publication Date
2018-09-06
Published Version
ISSN
2227-9717
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PII: pr6090160, Publication Type: Journal Article
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LAPSE:2019.0470
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doi:10.3390/pr6090160
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Apr 8, 2019
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Calvin Tsay
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