The startup process occurs frequently for pumped storage units. During this process, the rotating rate that changes rapidly and unsteady flow in runner cause the complex dynamic response of runner, sometimes even resonance. The sharp rise of stress and the large-amplitude dynamic stresses of runner will greatly shorten the fatigue life. Thus, the study of start-up process in turbine mode is critical to the safety operation. This paper introduced a method of coupling one dimensional (1D) pipeline calculation and three-dimensional computational dynamics (3D CFD) simulation to analyze transient unsteady flow in units and to obtain more accurate and reliable dynamic stresses results during start up process. According to the results, stress of the ring near fixed support increased quickly as rotating rate rose and became larger than at fillets of leading edge and band in the later stages of start-up. In addition, it was found that dynamic response can be caused by rotor stator interaction (... [more]

This article aims to develop a proton exchange membrane (PEM) electrolyzer emulator. This emulator is realized through an equivalent electrical scheme. It allows taking into consideration the dynamic operation of PEM electrolyzers, which is generally neglected in the literature. PEM electrolyzer dynamics are reproduced by the use of supercapacitors, due to the high value of the equivalent double-layer capacitance value. Steady-state and dynamics operations are investigated in this work. The design criteria are addressed. The PEM electrolyzer emulator is validated by using a 400-W commercial PEM electrolyzer. This emulator is conceived to test new DC-DC converters to supply the PEM ELs and their control as well, avoiding the risk to damage a real electrolyzer for experiment purposes. The proposed approach is valid both for a single cell and for the whole stack emulation.

Traditional wisdom suggests that the interchangeable design in process system engineering, such as modularity or commonality design, can lower the manufacturing cost and act as a revenue driver. Moreover, the interchangeable design will be efficient in both assembling for new production and disassembling for remanufacturing. As such, interchangeable design confronted remanufacturing processing often involves a balance of revenue from cost drivers and cannibalization effects from remanufacturing. Therefore, this paper studies how the original equipment manufacturers’ (OEMs’) interchangeable design impacts the remanufacturing decisions, as well as the economy and environment. Specifically, we develop two theoretical models, in which an OEM makes a strategic choice relating to design interchangeability when the remanufacturing operations are undertaken by itself (Model O) or outsourced to third-party remanufacturers (Model T). This study finds that, although the optimal level of interchan... [more]

Dissolved oxygen plays a key role in microalgal growth at high density. This effect was so far rarely quantified. Here we propose a new model to represent the combined effect of light, oxygen concentration and temperature (LOT-model) on microalgae growth. The LOT-model introduces oxygen concentration in order to represent the oxidative stress affecting the cultures, adding a toxicity term in the expression of the net growth rate. The model was validated with experimental data for several species such as Chlorella minutissima, Chlorella vulgaris, Dunaliella salina, Isochrysis galbana. It successfully predicted experimental records with an average error lower than 5.5%. The model was also validated using dynamical data where oxygen concentration varies. It highlights a strong impact of oxygen concentration on productivity, depending on temperature. The model quantifies the sensitivity to oxidative stress of different species and shows, for example, that Dunaliella salina is much less aff... [more]

Tripartite motif 21 (TRIM21) plays an important role in hepatocellular carcinoma (HCC). However, the gene polymorphisms of TRIM21 in HCC is not as well known. In this study, two single nucleotide polymorphisms (SNPs) in the TRIM21 gene, rs4144331, and re915956, were selected to investigate correlations between these SNPs and susceptibility to HCC. Two SNPs of the TRIM21 gene from 1196 controls without cancer and 394 HCC patients were analyzed using real-time polymerase chain reaction. These results were further analyzed to expound the associations between these TRIM21 polymorphisms and the risk of HCC as well as the impact of these SNPs on clinicopathological characteristics of HCC. After adjustment for other covariants, we observed that that younger patients (<65 years) with the TRIM21 rs915956 A allele had a probability of HCC (AOR = 3.153, 95% CI: 1.315−7.516, p = 0.010). Moreover, patients with a smoking habit who carried the T allele of rs4144331 had more probability of HCC (AO... [more]

Biorefinery, which utilizes carbon-neutral biomass as a resource, is attracting attention as a significant alternative in a modern society confronted with climate change. In this study, spent coffee grounds (SCGs) were used as the feedstock for lactic acid fermentation. In order to improve sugar conversion, alkali pretreatment was optimized by a statistical method, namely response surface methodology (RSM). The optimum conditions for the alkali pretreatment of SCGs were determined as follows: 75 °C, 3% potassium hydroxide (KOH) and a time of 2.8 h. The optimum conditions for enzymatic hydrolysis of pretreated SCGs were determined as follows: enzyme complex loading of 30-unit cellulase, 15-unit cellobiase and 50-unit mannanase per g biomass and a reaction time of 96 h. SCG hydrolysates were used as the carbon source for Lactobacillus cultivation, and the conversions of lactic acid by L. brevis ATCC 8287 and L. parabuchneri ATCC 49374 were 40.1% and 55.8%, respectively. Finally, the maxi... [more]

A hydrothermal pre-treatment has been developed to improve sewage sludge quality or to produce low nitrogen biocrude via hydrothermal liquefaction (HTL) in a subsequent step. The mild hydrothermal pre-treatment (150 °C) step was performed with deionized water, sulfuric acid (0.5 M), or citric acid (0.5 M) to solubilize nitrogen containing compounds in the aqueous supernatant. Downstream, the residual solid material was liquefied with the addition of sodium carbonate via hydrothermal liquefaction (350 °C). The pre-treatment with citric acid transferred up to 66.7 wt. % of nitrogen into the aqueous supernatant, while 62.0 wt. % of carbon was recovered in the solid. Due to the pre-treatment lipids retained in the sewage sludge solid, which increased the favored biocrude yield up to 42.9 wt. % and the quality evaluating value H/Ceff ratio significantly to 1.48. Multi-method characterization of the resulted biocrude samples showed a lower concentration of N-heterocycles, while long-chain al... [more]

The objective of the study was to investigate a more effective use of commercially available biomass pellets (wheat straw, wood, peat) using microwave pretreatment to improve heat production. Pellets were pretreated using the originally designed microwave torrefaction device. The effects of microwave (mw) pretreatment were quantified, providing measurements of the weight loss and elemental composition of pellets and estimating the effect of mw pretreatment on their porosity, surface area and calorific values at pretreatment temperatures of T = 448−553 K. Obtained results show that the highest structural variations and elemental composition during mw pretreatment were obtained for wheat straw pellets, with an increase in reactivity, a decreasing in the duration of the thermal decomposition by about 40% and an increase in the yield of combustible volatiles. Increased reactivity of pretreated pellets enhanced the ignition and burnout of volatiles, decreasing the duration of the burnout of... [more]

In this paper, an arc was drawn over ten years of research activities from three chairs of the Montanuniversitaet Leoben, as well as industrial partners. The superior objective of this research effort was to develop a wet-mechanical process for the recovery of polyolefin concentrates (90 wt% polyolefins) from mixed waste fraction for use in chemical recycling and to advance this new technology to commercial maturity. As a bridge technology, it would close the gap between state-of-the-art dry processing of mixed plastic waste materials and chemical plastic recycling via thermo-chemical conversion. The methods used were mainly tested in a lab-scale plant with a throughput capacity of 50 to 200 kg/h depending on the bulk density of the used feedstock. Further studies for the treatment and usage of the main products and by-products, as well as chemical analyses of them, were completed during the investigation. Within these series of tests, polyolefin concentrates, which satisfied the requi... [more]

Supercritical carbon dioxide + ethanol (SC-CO2+EtOH) extraction, was employed as a deflavoring method to improve the sensory properties of pea flours. Furthermore, the impacts of particle size along with extraction on volatile profile and sensory attributes of pea flours were investigated using multiple approaches. These included headspace solid-phase microextraction-gas chromatography (HS-SPME-GC), GC-olfactometry (GC-O), and quantitative descriptive analysis (QDA) using a trained sensory panel. Total volatile contents of non-deflavored and deflavored whole pea flour and its fractions were in the range of 7.1 ± 0.3 to 18.1 ± 1.0 µg/g and 0.4 ± 0.1 to 2.7 ± 0.4 µg/g, respectively. The GC-O system showed that the total volatile intensity was in the range of 14.5 to 22.0 and 0 to 3.5, for non-deflavored and deflavored pea flours, respectively. Volatile analyses indicated that 1-hexanol, 1-octanol, 1-nonanol, nonanal, and 2-alkyl methoxypyrazines were major off-aroma compounds. Most off-a... [more]

The development of a bio-based economy has its foundations in the development of efficient processes to optimize biomass potential. In this context there are a multitude of molecules that can be either synthetized or recovered from biomass, among those the so-called 12 building-blocks reported by the US Department of Energy. Even if their identification and importance is clearly defined, research efforts concerning the purification or separation of these platform molecules are limited. To fill this gap, different configurations for the purification of levulinic acid recovered from lignocellulosic biomass are examined and compared in this work. In particular, hybrid configurations obtained by the combination of liquid-liquid extraction and distillation have been considered. It was demonstrated how a deep understanding of the subspace including all extraction-assisted simple column distillation configurations represents a fundamental step in the synthesis of different process alternative... [more]

The integration of real time release testing into an advanced process control (APC) concept in combination with digital twins accelerates the process towards autonomous operation. In order to implement this, on the one hand, measurement technology is required that is capable of measuring relevant process data online, and on the other hand, a suitable model must be available to calculate new process parameters from this data, which are then used for process control. Therefore, the feasibility of online measurement techniques including Raman-spectroscopy, attenuated total reflection Fourier transformed infrared spectroscopy (ATR-FTIR), diode array detector (DAD) and fluorescence is demonstrated within the framework of the process analytical technology (PAT) initiative. The best result is achieved by Raman, which reliably detected mAb concentration (R2 of 0.93) and purity (R2 of 0.85) in real time, followed by DAD. Furthermore, the combination of DAD and Raman has been investigated, which... [more]

Although many benefits drawn from beer consumption are claimed, the epidemiological records are contradictory with respect to cancer prevention. The purpose of this study was to investigate the possible health-related activities involving genome safety and the ageing processes of two types of lyophilised ale beers (blond and stout), as well as two of their bioactive compounds (tyrosol and iso-alpha humulone). A multipurpose trial set of in vivo toxicity, antitoxicity, mutagenicity, antimutagenicity, lifespan and healthspan assays using Drosophila melanogaster were used. In parallel, several in vitro assays were designed using the cancer cell line HL-60 in order to establish the possible chemopreventive activity of the selected substances, where epigenetic modulation of DNA methylation changes, clastogenic activity and tumour cell inhibition growth were evaluated. The safety of the four substances was confirmed: lyophilised blond ale beer (LBAB), lyophilised stout ale beer (LSAB), tyros... [more]

Proportional−integral−derivative (PID) control remains the primary choice for industrial process control problems. However, owing to the increased complexity and precision requirement of current industrial processes, a conventional PID controller may provide only unsatisfactory performance, or the determination of PID gains may become quite difficult. To address these issues, studies have suggested the use of reinforcement learning in combination with PID control laws. The present study aims to extend this idea to the control of a multiple-input multiple-output (MIMO) process that suffers from both physical coupling between inputs and a long input/output lag. We specifically target a thin film production process as an example of such a MIMO process and propose a self-tuning two-degree-of-freedom PI controller for the film thickness control problem. Theoretically, the self-tuning functionality of the proposed control system is based on the actor-critic reinforcement learning algorithm.... [more]

Uncertainty and variability are inherent to pile design and consequently, there have been considerable researches in quantifying the reliability or probability of failure of structures. This paper aims at examining and comparing the applicability and adaptability of Minimax Probability Machine Regression (MPMR), Emotional Neural Network (ENN), Group Method of Data Handling (GMDH), and Adaptive Neuro-Fuzzy Inference System (ANFIS) in the reliability analysis of pile embedded in cohesionless soil and proposes an AI-based prediction method for bearing capacity of pile foundation. To ascertain the homogeneity and distribution of the datasets, Mann−Whitney U (M−W) and Anderson−Darling (AD) tests are carried out, respectively. The performance of the developed soft computing models is ascertained using various statistical parameters. A comparative study is implemented among reliability indices of the proposed models by employing First Order Second Moment Method (FOSM). The results of FOSM sho... [more]

In this study, we examined the effect of ethanolic extract of Salicornia herbacea (ESH), isorhamnetin 3-O-glucoside (I3G), quercetin 3-O-glucoside (Q3G), quercetin, and isorhamnetin on α-glucosidase activity and glucose-stimulated insulin secretion (GSIS) in insulin-secreting rat insulinoma (INS-1) cells. A portion of the ethyl acetate fraction of ESH was chromatographed on a silica gel by a gradient elution with chloroform and methanol to provide Q3G and I3G. ESH, Q3G, and quercetin inhibited α-glucosidase activity, and quercetin (IC50 value was 29.47 ± 3.36 μM) inhibited the activity more effectively than Q3G. We further demonstrated that ESH, Q3G, quercetin, I3G, and isorhamnetin promote GSIS in INS-1 pancreatic β-cells without inducing cytotoxicity. Among them, I3G was the most effective in enhancing GSIS. I3G enhanced the phosphorylation of total extracellular signal-regulated kinase (ERK), insulin receptor substrate-2 (IRS-2), phosphatidylinositol 3-kinase (PI3K), Akt, and activa... [more]

Cubing machines in food processing have evolved over the years, which have made products like Star®, Knorr®, and Chicken® bouillon cubes become commercially available today, even in many parts of Africa. On the other hand, the fermented mesquite seed “okpeye” food condiment, traditionally produced by well-trained artisans and widely utilised in Nigeria, requires further product development in order to compete with bouillon cubes. Quality comparisons between the cubed “okpeye” condiment and commercially available bouillon cube products have not yet been reported. Therefore, cubing fabrication/costing and machine performance on African fermented “okpeye” condiment quality attributes, compared with commercial bouillon types, were done. The processing of mesquite seeds into the “okpeye” condiment resembled those of artisans. Bouillon cube products involved the Star®, Knorr®, and Chicken® types. Quality attributes involved proximate, micronutrient, phytochemical, and microbial aspects. Resu... [more]

Propylene glycol, also known as 1,2 propanediol, is one of the most important chemicals in the industry. It is a water-soluble liquid, considered by the U.S. Food and Drug Administration as safe to manufacture consumer products, including foodstuffs, medicines, and cosmetics. This chemical has essential properties, such as solvent, moisturizer, or antifreeze, in addition to a low level of toxicity. This paper aims to present the selection, simulation, and dimensioning of a trickle bed reactor at a laboratory scale. The sizing was validated with other authors. Two predictive models have been considered for reactor modeling, intrinsic kinetics and coupled intrinsic kinetics, along with mass transfer equations and the wetting of the catalyst particles. The model was implemented using Aspen Custom Modeler® (20 Crosby Dr. Bedford, MA 01730, EE. UU.) to study the reactor behavior in terms of conversion. The results show the profiles of different variables throughout the reactor and present h... [more]

During the transportation and packaging of low density polyethylene (LDPE) granular materials, fine dusts such as floccules, powder and fiber will be produced, which pollute the environment, affect product quality and generate fire hazards. In this work, the separation performance of fine dust and optimal operating conditions of an improved elutriator were investigated experimentally. Experiments were carried out to investigate the effects of air speed, feeding speed, and grid layout on the removal efficiency of fine particles. Experimental data showed that the separation efficiency of the novel elutriator ranged from 96% to 98.50%, which was more stable and an average of 51.44% higher than that of the original elutriator. By setting internals and improving the structure, the gas flow field in the equipment was regulated, the particle dispersion was intensified, and the static electricity was eliminated, which significantly improved the separation efficiency of fine dust.

A symmetrical NACA 0018 airfoil is often used in such applications as small-to-medium scale vertical-axis wind turbines and aerial vehicles. A review of the literature indicates a large gap in experimental studies of this airfoil at low and moderate Reynolds numbers in the previous century. This gap has limited the potential development of classical turbulence models, which in this range of Reynolds numbers predict the lift coefficients with insufficiently accurate results in comparison to contemporary experimental studies. Therefore, this paper validates the aerodynamic performance of the NACA 0018 airfoil and the characteristics of the laminar separation bubble formed on its suction side using the standard uncalibrated four-equation Transition SST turbulence model and the unsteady Reynolds-averaged Navier-Stokes (URANS) equations. A numerical study was conducted for the chord Reynolds number of 160,000, angles of attack between 0 and 11 degrees, as well as for the free-stream turbule... [more]

The application of white box models in digital twins is often hindered by missing knowledge, uncertain information and computational difficulties. Our aim was to overview the difficulties and challenges regarding the modelling aspects of digital twin applications and to explore the fields where surrogate models can be utilised advantageously. In this sense, the paper discusses what types of surrogate models are suitable for different practical problems as well as introduces the appropriate techniques for building and using these models. A number of examples of digital twin applications from both continuous processes and discrete manufacturing are presented to underline the potentials of utilising surrogate models. The surrogate models and model-building methods are categorised according to the area of applications. The importance of keeping these models up to date through their whole model life cycle is also highlighted. An industrial case study is also presented to demonstrate the app... [more]

Optimally designed functional foods are considered the most important part of a balanced and healthy diet. Goat meat nuggets, an otherwise healthy option, are packed with undesirable saturated and unsaturated fats. The present work suggests an optimal functional formulation to reduce the surplus fat content of goat meat nuggets by adding two optimally calculated functional ingredients, namely, fenugreek leaves (FL) and psyllium husk (PH). Response surface optimization was performed to determine the optimal content of the functional ingredients (FL and PH), resulting in minimum fat content without affecting the overall acceptability (OA) and other properties representing the taste and texture (e.g., ash content, pH, crude fiber content, and moisture content) of the nuggets. Functional additives at optimum levels successfully reduced the fat content of the weight-conserved nuggets by almost 39% compared with the control nuggets. Minimal and acceptable effects were observed regarding OA a... [more]

Muscular tissue regeneration may be enhanced in vitro by means of mechanical stimulation, inducing cellular alignment and the growth of functional fibers. In this work, a novel bioreactor is designed for the radial stimulation of porcine-derived diaphragmatic scaffolds aiming at the development of clinically relevant tissue patches. A Finite Element (FE) model of the bioreactor membrane is developed, considering two different methods for gripping muscular tissue patch during the stimulation, i.e., suturing and clamping with pliers. Tensile tests are carried out on fresh and decellularized samples of porcine diaphragmatic tissue, and a fiber-reinforced hyperelastic constitutive model is assumed to describe the mechanical behavior of tissue patches. Numerical analyses are carried out by applying pressure to the bioreactor membrane and evaluating tissue strain during the stimulation phase. The bioreactor designed in this work allows one to mechanically stimulate tissue patches in a radial... [more]

To foster a circular economy in line with compost quality assessment, a deep understanding of the fates of nutrients and carbon in the composting process is essential to achieve the co-benefits of value-added and environmentally friendly objectives. This paper is a review aiming to fill in the knowledge gap about the composting process. Firstly, a systematic screening search and a descriptive analysis were conducted on composting models involving the fates of Carbon (C), Nitrogen (N), Phosphorus (P) and Potassium (K) over the past decade, followed by the development of a checklist to define the gap between the existing models and target models. A review of 22 models in total led to the results that the mainstream models involved the fates of C and N, while only a few models involved P and K as target variables. Most of the models described the laboratory-scale composting process. Mechanism-derived models were relatively complex; however, the application of the fractionation of substrat... [more]

This study proposes a reliable inline PAT concept for the simultaneous monitoring of different product components after chromatography. The feed for purification consisted of four main components, IgG monomer, dimer, and two lower molecular weight components of 4.4 kDa and 1 kDa molecular weight. The proposed measurement setup consists of a UV−VIS diode-array detector and a fluorescence detector. Applying this system, a R2 of 0.93 for the target component, a R2 of 0.67 for the dimer, a R2 of 0.91 for the first side component and a R2 of 0.93 for the second side component is achieved. Root mean square error for IgG monomer was 0.027 g/L, for dimer 0.0047 g/L, for side component 1 0.016 g/L and for the side component 2 0.014 g/L. The proposed measurement concept tracked component concentration reliably down to 0.05 g/L. Zero-point fluctuations were kept within a standard deviation of 0.018 g/L for samples with no IgG concentration but with side components present, allowing a reliable det... [more]