Microbial bioformulations, due to their positive impact on the growth and development of plants, as well as the absence of harmful effects on the environment and humans, have a vast potential for mass introduction into agriculture. Assessing the quality of bioformulations, especially complex ones, is a difficult task. In this study, we show that high-throughput sequencing can be an effective tool for the quality control and safety of microbial bioformulations. By the method of high-throughput sequencing on the MiSeq platform, we studied 20 samples of commercially available microbial bioformulations. In parallel with this, bioformulations were studied by classical microbiological methods. The analysis showed the presence of extraneous undeclared bacterial genera by the manufacturer. Only 10% of the bioformulations fully corresponded to the commercial composition, and another 10% of the bioformulations did not contain the bacteria declared by the manufacturer in their composition at all.... [more]

Widely used in traditional medicine, sheep placenta extract (SPE) is known for its physiological effects such as wound healing, antioxidant, and anti-inflammatory properties. However, the effect of SPE on antiaging is still unclear. In this study, we investigated the effect of SPE on aging through the senescence-accelerated mouse prone 8 (SAMP8) strain. We designed an experiment using both male and female mice randomly divided into 4 groups (n = 10) as follows: Group A—control group; Group B—low-dose SPE (61.5 mg/kg BW/day); Group C—medium-dose SPE (123 mg/kg BW/day); and Group D—high-dose SPE (184.5 mg/kg BW/day). As a result of measuring the aging index parameters such as skin glossiness, spine lordosis, and kyphosis, it was found that the treatment of SPE lowered the aging index. In addition, we found that biochemical parameters such as lactic acid, glucose, ketone bodies, free fatty acids, tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6) were not changed in the experim... [more]

The pump as turbines (PATs) has been widely used in the petrochemical, seawater desalination, and mining industries. Volutes are critical components for flow guidance and energy conversion in the PATs. Therefore, its inner flow characteristic could significantly influence the hydraulic turbine system stability. To reveal the vortex evolution, pressure pulsation characteristics, and the interaction between the two in the volute of PATs, a single-stage cantilever hydraulic turbine is investigated by the numerical and experiment method. The effect of impeller rotation on vorticity distribution and pressure fluctuation intensity in volute is analyzed based on the numerical simulation results. By clarifying the frequency components corresponding to local high amplitude vorticity and pressure pulsations, the relationship between vortex evolution and pressure pulsations is established. The results showed that the dominant frequency of pressure pulsation in the circumferential direction of the... [more]

The improper disposal of flower waste from cultural activities is one of the main challenges in certain countries such as India. If the flower waste is not managed properly, it causes a number of environmental issues. Therefore, various technologies have been developed to transform flower waste into value-added products. To integrate multiple technologies holistically to maximise the energy and material recovery, an integrated flower-waste biorefinery is required. Since there are a wide range of technologies available that can convert the waste into multiple products, there is a need to develop a systematic approach to evaluate all the technologies. This research proposes a systematic approach to synthesise an integrated flower-waste biorefinery based on different optimisation objectives, e.g., maximum economic performance and minimum environmental impact. Due to the conflicting nature between the two objectives, a fuzzy optimisation approach has been adapted to synthesise a sustainabl... [more]

The climate change impact associated with greenhouse gas emissions is a major global concern. This work investigates perovskite compounds for oxygen separation from air to supply oxygen to oxyfuel energy systems to abate these significant environmental impacts. The perovskites studied were Me0.5Sr0.5Co0.8Cu0.2O3−δ (MeSCC) where the A-site substitution was carried out by four different cations (Me = Ca, Mg, Sr, or Ba). SEM analysis showed the formation of small particle (<1 µm) aggregates with varying morphological features. XRD analysis confirmed that all compounds were perovskites with a hexagonal phase. Under reduction and oxidation reactions (redox), Ba and Ca substitutions resulted in the highest and lowest oxygen release, respectively. In terms of real application for oxygen separation from air, Ba substitution as BaSCC proved to be preferable due to short temperature cycles for the uptake and release of oxygen of 134 °C, contrary to Ca substitution with long and undesirable te... [more]

Despite the development of many antibiotics, excessive inflammation caused by endotoxins is still a subject of interest to biomedical researchers. The hyper-inflammatory response of macrophages activated by endotoxins is an important topic in the development of natural product-based anti-inflammatory drugs. Conioselinum tenuissimum, a perennial herb of the family Apiaceae, contains levistolide A, demethylsuberosin, and fraxetin. One of the synonyms of Conioselinum tenuissimum is Angelica tenuissima. The objective of this study was to determine the effects of Conioselinumtenuissimum root water extract (AT) on the hyper-inflammatory responses of macrophages activated by endotoxin (lipopolysaccharide; LPS) and the mechanisms involved in such effects. Levels of cytokines, nitric oxide (NO), hydrogen peroxide, and cytosolic calcium in LPS-activated RAW 264.7 murine macrophages were evaluated by the multiplex cytokine assay (MCA), Griess reagent assay (GRA), dihydrorhodamine 123 assay (DHR),... [more]

Extractive distillation has great significance for the separation of ethylbenzene from C8 aromatic hydrocarbons. Herein, a distillation process for the separation of ethylbenzene was designed using methyl phenylacetate as an extractant. A genetic algorithm (GA) was used to evaluate the economic and environmental factors of the process, and Aspen Dynamic was used to assess the dynamic performance. The sequential optimization method was used to obtain the initial process parameters. Then, the total annual cost and CO2 emissions were minimized by NSGA-III to increase the economic and environmental benefits. To enhance the search performance of GA, the mutation probability and crossover probability were studied and adjusted. The optimal total annual cost and CO2 emissions were 11.7% and 23.7% lower than those of the initial process. Based on a steady process, two control strategies, which were the flow rate of the recycling solvent controlled by entrainer makeup flow rate (CS1) and the bot... [more]

The transport and prediction of hexavalent chromium (Cr(VI)) contamination in “slag−soil−groundwater” is one with many uncertainties. Based on the column experiments, a migration model for Cr(VI) in the slag−soil−groundwater system was investigated. The hydraulic conductivity (Kt), distribution coefficient (Kd), retardation factor (Rd), and other hydraulic parameters were estimated in a laboratory. Combining these hydraulic parameters with available geological and hydrogeological data for the study area, the groundwater flow and Cr(VI) migration model were developed for assessing groundwater contamination. Subsequently, a Cr(VI) migration model was developed to simulate the transport of Cr(VI) in the slag−soil−groundwater system and predict the effect of three different control programs for groundwater contamination. The results showed that the differences in the measured and predicted groundwater head values were all less than 3 m. The maximum and minimum differences in Cr(VI) between... [more]

Evaluating the degree of coordination among regional carbon emission systems is key to achieving an earlier carbon peak and carbon neutrality. However, quantifying the co-evolution of carbon emissions among regions is challenging. Therefore, we propose a data-driven method for evaluating the synergetic development of the regional carbon emission composite system. First, the proposed method employs relevant data to calculate the carbon emissions and carbon emission intensity of each subsystem within the region to describe the temporal trends. The inverse entropy weight method is then used to assign weight to each order parameter of the subsystem for data processing. Then, we perform synergetic development assessment of the composite system to measure the order degree of each subsystem, the degree of synergy among subsystems, and the overall synergetic degree of the temporal evolution of carbon emissions between regions. Finally, the evaluation results can be used to suggest measures for... [more]

Since the beginning of this century, the world has experienced a growing need for enabling techniques and more environmentally friendly protocols that can facilitate more rational industrial production. Scientists are faced with the major challenges of global warming and safeguarding water and food quality. Organic solvents are still widely used and seem to be hard to replace, despite their enormous environmental and toxicological impact. The development of water-based strategies for the extraction of primary and secondary metabolites from plants on a laboratory scale is well documented, with several intensified processes being able to maximize the extraction power of water. Technologies, such as ultrasound, hydrodynamic cavitation, microwaves and pressurized reactors that achieve subcritical water conditions can dramatically increase extraction rates and yields. In addition, significant synergistic effects have been observed when using combined techniques. Due to the limited penetrati... [more]

The presence of tens of Clostridium botulinum spores per gram of honey can cause infantile botulism. Thermal treatment is insufficient to inactivate these resistant forms. This study explored the effectiveness of supercritical CO2 (scCO2) on its own and combined with lemon (LEO), clove (CLEO), and cinnamon (CEO) essential oils on the inactivation of Clostridium sporogenes (CECT 553) as a surrogate of Clostridium botulinum. In water, the degree of inactivation at 10 MPa after 60 min increased with the increasing temperature, reducing the population by 90% at 40 °C and by 99.7% at 80 °C. In contrast, when applied to honey, scCO2 did not inactivate Clostridium spores satisfactorily at temperatures below 70 °C, which was related to the protective effect of honey. Meanwhile, scCO2 modified with CEO (<0.4% mass) improved the inactivation degree, with a 1.3-log reduction achieved at 60 °C. With this same mixture, a reduction of 3.7 logs was accomplished in a derivative with 70% moisture. H... [more]

Airborne fungi emitted from livestock manure composting plants are one of the major harmful factors causing respiratory disease for workers and nearby residents. Their generation of emissions is relatively high compared to other workplaces. This study investigated the emission characteristics of airborne fungi generated in livestock manure composting plants and utilized them as basic data to prevent workers’ health. The livestock manure composting plants selected for the survey in this study were according to the fermentation mode, including screw type, rotary type and natural dry type. The field evaluation period was from September 2019 to August 2020 and was surveyed monthly. The equipment for collecting airborne fungi was a six-stage cascade impactor. An analysis of the quantification and qualification of airborne fungi was conducted through a culture method and identification technique, respectively. The mean levels of airborne fungi in livestock manure composting plants were 1143... [more]

In the last years, the demand for new eco-friendly ingredients has increased on the cosmetic market. Consumers are more aware of sustainable principles and, simultaneously, more conscious regarding the skin aging process. Chestnut (Castanea sativa) shells are a food by-product produced in high quantities in Europe. This waste has been described as a rich source of phenolic compounds with skin biological effects, such as antioxidant, antiradical, and anti-inflammatory activities. Despite the huge number of assays reporting the richness of chestnut shell extracts in bioactive compounds as well as the development of cosmetic formulations containing these extracts, no in vivo assays have assessed their clinical efficacy in human volunteers. The aim of this study was to evaluate the effect on skin face parameters of a formulation containing a chestnut shell extract in human volunteers (n = 22) who applied the product twice per day, for 56 days. For that, biophysical techniques, including Co... [more]

In this paper, the heat transfer and flow characteristics of a helically coiled tube with twisted elliptical in a low Reynolds number (Re = 500−3000) flow were investigated numerically. The working fluid flowed in a laminar regime. Numerical results were compared with empirical correlations in the existing literature, demonstrating the accuracy of the analysis in this study. Firstly, we investigated the effects of geometric parameters and Reynolds number on the heat transfer and flow characteristics. The results showed that lower twist pitch length and semi-major axis length could induce sufficient fluid mixing and a larger temperature gradient near the tube wall, enhancing the heat exchange and producing larger friction resistance. Overall, the heat transfer performances were improved by about 1.04−1.21 times and 1.02−1.23 times for different semi-major axis lengths and different twist pitch lengths, respectively, compared to the smooth helical coil. Secondly, by changing the working... [more]

An axial flow pump is a kind of high-specific revolution vane pump that has the characteristics of large flow, low head, and high efficiency. Due to its unique properties, it is widely used in large water diversion projects, such as the South-to-North Water Diversion Project. However, during the operation of the pump, some fish enter the axial flow pump together with the water flow through the screen before the entrance of the pump station. Consequently, some fish are inevitably damaged or even die in the process of traversing through the pump. Meanwhile, the decay of dead fish directly affects the quality of water, hence, posing serious ecological pollution and destabilizing the ecological balance. Therefore, understanding the dynamics of axial flow pumps in relation to fish species in water bodies for biodiversity and ecosystem services remain vital for nature conservation. In this paper, the impact of damage of the model pump on fish is exhaustively investigated according to the the... [more]

The chemical co-precipitation method was used to prepare magnetically separable Fe−Mn oxide composites, and the degradation of p-chloroaniline (PCA) using MnFe2O4 activated peroxydisulfate (PDS). The MnFe2O4 catalyst exhibited highly catalytic activity in the experiments. XRD, FTIR, SEM and TEM were used to characterize the catalytic materials. MnFe2O4 calcined at 500 °C was more suitable as a catalytic material for PCA degradation. The elevated reaction temperature was beneficial to the degradation of PCA in neutral pH solution. The reaction mechanism of the MnFe2O4 catalyzed oxidative degradation of PCA by PDS was investigated by free radical quenching experiments and XPS analysis. The results showed that sulfate radicals (SO4•−), hydroxyl radicals (•OH) and singlet oxygen (1O2) may all be participated in the degradation of PCA. XPS spectra showed that the electron gain and loss of Mn2+ and Fe3+ was the main cause of free radical generation. The possible intermediates in the degradat... [more]

sp. is a microorganism cultured for producing docosahexaenoic acid (DHA). Genome-scale metabolic modeling (GEM) is a promising technique for describing gen-protein-reactions in cells, but with still limited industrial application due to its complexity and high computation requirements. In this work, we simplified GEM results regarding the relationship between the specific oxygen uptake rate (−rO2), the specific growth rate (µ), and the rate of lipid synthesis (rL) using an evolutionary algorithm for developing a model that can be used by a soft sensor for fermentation monitoring. The soft sensor estimated the concentration of active biomass (X), glutamate (N), lipids (L), and DHA in a Schizochytrium sp. fermentation using the dissolved oxygen tension (DO) and the oxygen mass transfer coefficient (kLa) as online input variables. The soft sensor model described the biomass concentration response of four reported experiments characterized by different kLa values. The average range normali... [more]

The aim of the study was to develop a methodology for the acquisition of skin images in visible light in a repeatable manner, enabling an objective assessment and comparison of the skin condition before and after a series of IPL treatments. Thirteen patients with erythematous lesions, vascular skin and/or rosacea were examined. Treatments aimed at reducing the erythema were carried out using the Lumecca™ (InMode MD Ltd., Yokneam, Israel) The research used the FOTOMEDICUS image acquisition system (Elfo, Łódź, Poland). The RGB images were recorded and decomposed to individual channels: red, green and blue. Then, the output image (RGB) and its individual channels were transformed into images in shades of gray. The GLCM and QTDECOMP algorithms were used for the quantitative analysis of vascular lesions. Image recording in cross-polarized light enables effective visualization of vascular lesions of the facial skin. A series of three treatments using the IPL light source seems to be sufficie... [more]

Saudi Arabia is one of the major producers of date (Phoenix dactylifera) fruit. Date fruit flesh is considered a healthy food due to the presence of natural antioxidants. Green and innovative supercritical fluid (SFE, 52.5 °C temperature, 27.50 MPa pressure, 5 mL CO2/min flow rate) and subcritical (SubCO2, 250 extraction cycles, 29 °C temperature, 6.8 MPa, 12 h, ethanol solvent) extraction techniques were used to produce flesh extracts from four Saudi date fruits (Sukari (SKFE), Ambara (AMFE), Majdool (MJFE) and Sagai (SGFE)), and extracts prepared using 6 h Soxhlet extraction at 70 °C for 16 h using n-hexane as solvent, were taken as control. SFE produced the highest (p < 0.05) extract yields, whereas the SubCO2 method recovered significantly higher (p < 0.05) amounts of phytochemicals. Total phenolics (186.37−447.31 mg GAE/100 g), total flavonoids (82.12−215.28 mg QE/100 g), total anthocyanins (0.41−1.34 mg/100 g), and total carotenoid (1.24−2.85 mg BCE/100 g) were quantified i... [more]

Failure modes and effects analysis (FMEA) is a systematic approach that focuses on evaluating critical disturbances in a system. However, traditional FMEA has its own drawbacks, such as invalid computations and ambiguous priority definitions, which lead to many constraints in the application of complex production processes, especially in job shops with various resources. Therefore, this paper proposes an analytic disturbance prediction method for job shop with multiple resources and multiple evaluation indexes, which combines the vector computing techniques, FMEA, and fuzzy analytic hierarchy process (FAHP). In contrast to other work, this paper focuses on the establishment of FMEA mathematical model to improve the readability of multi-resource disturbance risk results. To this end, the projection of the disturbance vector is visualized to reduce repeated calculation results, triangles and trapezoids are used as membership functions to improve the accuracy of weight, and the differenti... [more]

The biodegradation behavior of a ternary alloy Mg-4Li-1Ca (LX41) was investigated. Preliminary studies of the alloys showed that its property profile may qualify it as a material of choice for bioresorbable bone implants. However, no data on the in vivo behavior of the alloy were available to date. Here we report the results on the in vivo response of juvenile growing rats to implantation over a period of 24 weeks based on micro-computed tomography and histology examination. A comparison with an established Mg-Zn-Ca alloy revealed a higher biodegradation rate of LX41. However, LX41 implants were well integrated, and their faster degradation did not negatively affect bone in-growth and morphology. While lower degradation rates are still desirable, especially at the initial stage of bioresorption, the results obtained suggest that the moderate degradation rates observed will not be detrimental to long-term outcomes of LX41 implantation.

An oblique flow pump is widely used in farmland irrigation and drainage, water transfer projects, thermal power generation, and other fields. However, unstable flow factors in the oblique flow pump easily lead to pump vibration and noise. To improve the stability of pump operation and optimize the operating environment, it is necessary to study the flow-induced noise characteristics of oblique flow pumps. In this paper, CFD and noise simulation software are used to calculate the flow field and sound field of the oblique flow pump. The internal flow characteristics and flow-induced noise characteristics of the oblique flow pump were studied. The results show that when the flow rate of the oblique flow pump deviates from the optimal operating point, especially in the small flow rate, due to the phenomena of backflow and flow separation, more high-energy vortices are produced in the flow channel, and the vortices are distributed in a wide area, which will cause greater flow-induced noise.... [more]

Traditional wastewater treatments involve expensive mechanical and physiochemical methods, so researchers have been developing cost-effective, sustainable technologies that use enzymes to produce higher quality effluents and recover more energy and nutrients from wastewater. A thermostable, alkaline, and solvent-tolerant lipase was partially purified from thermophilic Bacillus stearothermophilus. The lipase displayed maximum activity at 50 °C and pH 11.0 and catalyzed both short- and long-chain triacylglycerols at similar rates. B. stearothermophilus lipase also exhibited high stability when incubated at 40 °C for 1 h with anionic and non-ionic surfactants. Studies show that thermostable enzymes can be improved through immobilization and modification of other reaction conditions. Therefore, B. stearothermophilus lipase was immobilized through adsorption on CaCO3, Celite 545, and silica gel with the CaCO3 support producing the best adsorption rate (89.33%). The optimal initial lipase ac... [more]

is a plant with a high biomass and heavy metal tolerance, which is a good candidate for phytoremediation. Pot experiments were conducted to compare the growth response, Pb enrichment ability, and the effect on Pb speciation of two ecotypes of M. floridulus from the Dabaoshan Mining Area and the non-mining area of Boluo County, Huizhou, in soils with different Pb contents. The results showed that two ecotypes of M. floridulus had different growth responses to Pb concentrations in soil. Under a low concentration of Pb (100 mg·kg−1) treatment, the aboveground biomass of the non-mining area plant ecotype was significantly affected, while the plants with the mining area ecotype were not significantly affected. When the concentration of Pb increased, the aboveground biomass of the non-mining ecotype was 30.2−41.1% of the control, while that of the mining ecotype was 57.8−65.0% of the control. The root biomass of the non-mining ecotype decreased with the increase of treatment concentration, a... [more]

In this study, the bioactive components in the stem of Hibiscus taiwanensis S. Y. Hu were extracted by supercritical carbon dioxide (SC-CO2), and the inhibition activity of the extract tyrosinase was analyzed. When the particle size of the powder was controlled to be 50 mesh, the effects of temperatures (40, 45, 50, 55, and 60 °C), pressures (15, 20, 25, 30, and 35 MPa), concentrations of entrainment agent (ethanol) (60, 80, and 95%) and CO2 flow rates (80, 100, 120, and 140 L/min) on the extraction rate, respectively, were studied with the single-variable method. The optimal parameters included an extraction temperature of 55 °C, an extraction pressure of 30 MPa, 80% ethanol as the entrainment agent, and the CO2 flow rate of 100 L/min. Under the optimal conditions, the extraction rate of Hibiscus taiwanensis S. Y. Hu reached more than 0.5% within 2 h. At the same time, the chemical compositions of the extract were investigated by using high-resolution liquid chromatography-mass spectr... [more]