Sensors and process control systems are essential for process automation and optimization. Many sectors have adapted to the Industry 4.0 paradigm, but copper smelters remain hesitant to implement these technologies without appropriate justification, as many critical functions remain subject to ground operator experience. Recent experiments and industrial trials using radiometric optoelectronic data acquisition, coupled with advanced quantitative methods and expert systems, have successfully distinguished between mineral species in reactive vessels with high classification rates. These experiments demonstrate the increasing potential for the online monitoring of the state of a charge in pyrometallurgical furnaces, allowing data-driven adjustments to critical operational parameters. However, the justification to implement an innovative control system requires a quantitative framework that is conducive to multiphase engineering projects. This paper presents a unified quantitative framewor... [more]

Xylindein, a stable quinonic blue-green fungal pigment, has shown potential for use not only as a colorant but also as an (opto)electronic material. As no method presently exists to synthesize the pigment, organic production by slow-growing fungi from the genus Chlorociboria is the only method to obtain it. This has resulted in limited quantities of impure xylindein, hampering research. In order to improve quantity and quality of pigment for optoelectronic applications, speed of xylindein production by Chlorociboria aeruginosa and its relative purity were compared across liquid and solid-state fermentation conditions on selected nutrient sources. Liquid 2% malt shaking cultures produced the same amount of pigment in 5 weeks that previous testing produced in 2 months. Xylindein generation speed, purity, and conductive properties of produced pigment for (opto)electronics was then compared between two Chlorociboria species native to North America, Chlorociboria aeruginosa and Chlorocibori... [more]

The aim of this study was to synthesize, characterize, and evaluate neoteric imine compounds for antimicrobial activity and hemocompatibility. Four compounds were synthesized using 3-thiophene carboxaldehyde, ethanol, amine, and acetic acid. The compounds were characterized using nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), and powder X-ray diffraction (PXRD). A solubility study was conducted with various solvents and surfactants at 40 °C. An in vitro antimicrobial assay was performed against bacterial and fungal strains to determine the zone of inhibition and minimum inhibitory concentrations. Finally, an in vitro hemolysis study was conducted using rat erythrocytes. The structure of the compounds was confirmed by NMR, FTIR corroborated their functional group attributes, DSC determined their enthalpies of fusion and fusion temperatures, and PXRD confirmed their crystalline nature. These compound... [more]

The working principle and motion process of an aviation wet clutch are analyzed. The initial velocity before the friction pair engaged is solved. The transient Reynolds equation is modified, and an oil film bearing capacity model and a micro-convex bearing capacity model are derived. The film thickness equation between N friction pairs and a pressure-plate is derived. A dynamic engaged model of springs, pistons, friction pairs, and pressure plates are established. The torque balance equation is established of two pairs of friction pairs. The friction torque, rate of change in the oil film, and law of relative change in speed are obtained. The results demonstrate that the spring preload and the viscosity of the lubricating oil have a significant influence on the engagement characteristics. Increasing the quality of the friction plate will reduce the time of engagement, whereas the quality of the friction plate has slight effect on the friction torque characteristics and oil film thickne... [more]

For the last twenty years, polymer hybrid nanocomposites have enjoyed unflagging interest from numerous scientific groups and R&D departments, as they provide notable enhancement of properties, even at low nanofillers’ content. Their performance results from many factors, the most important of which is the uniform distribution in the entire volume of the matrix, that still is very challenging, but is the right choice of two types of nanoparticles that can lead to an increase of dispersion stability and even more uniform distribution of fillers. The incorporation of two types of nanofillers, especially when they differ in aspect ratio or chemical nature, allows to additively reduce the price of the final composite by replacing the more expensive filler with the cheaper one, or even synergistically improving the properties, e.g., mechanical, thermal, and barrier, etc., that can extend their usage in the industry. Despite numerous review papers on nanocomposites, there is no review on how... [more]

 In the present report, we provide the results of the molecular biology studies of spiroheterocyclic hybrids, where the derivatives are found to possess potential anticancer activity towards cancer cells. A series of spiroxindole−pyrrolidine heterocyclic hybrids were evaluated for cell viability and proliferation against HepG2 cancer cells at concentrations in the range of 12.5−200 µg/mL over two different time periods of 24 and 48 h. In addition, the highly active compounds were also verified for their behavior towards noncancer cells (L929 cells), and it was found that the tested derivatives were not aggressive due to the observation of only limited cell loss, as compared to the cancer cells. Further analysis of the observed toxicity mechanism showed the apoptotic pathway was mediated by oxidative stress, with the involvement of caspases.

The main objective of the present paper is to compare, by means of a problem leading to a closed-form solution, the qualitative behavior of solutions based on three strain hardening laws: Swift’s law, Ludwik’s law, and Voce’s law. The boundary value problem involves the maximum friction law as one of the boundary conditions. Such features of the solutions as nonexistence and singularity are emphasized. An important feature of Swift’s and Ludwik’s laws is that the equivalent stress approaches infinity as the equivalent strain approaches infinity. On the contrary, Voce’s law involves saturation stress as one of the constitutive parameters. This qualitative difference in the equivalent stress behavior as the equivalent strain approaches infinity results in the qualitative difference in solutions’ behavior. In particular, Swift’s and Ludwik’s hardening laws are compatible with the regime of sticking independently of other conditions. In the case of Voce’s law, the solution under sticking c... [more]

The NOx reduction in the iron ore pelletizing process becomes an important environmental concern owing to its role in the formation of photochemical smog and acid rain. Thus, it is essential to develop new technologies for reducing NOx emissions in order to contribute to the cleaner production of pellets. In this paper, NOx reduction by advanced reburning ingrate-rotary kiln for oxidized pellet production was performed on a laboratory-scale gas kiln. Temperature and NH3/NOx molar ratio (NSR) were the key factors affecting the reduction of NOx. A better denitrification effect can be obtained on flus gas with higher initial NOx concentration, at temperature = 900 °C, NSR = 1.2, and reaction time exceeds one second. NOx reduction rate had reached 55−65% when the initial NOx concentration was above 400 ppm, and exceeds 70% when the initial NOx concentration was around 680 ppm. Urea solution has the best denitrification effect compared with NH3·H2O and NH4HCO3 solution. As for additives, th... [more]

Currently, cellulose nanostructures are among the most promising structures, and extensive work in materials and biotechnology industries is aimed at identifying an efficient process of production. Even when production at the laboratory scale is successful, crucial aspects of increased commercial applications for cellulose nanostructures are linked to large-scale production. Large-scale production requires a balance between the cost of the culture medium and product value. Therefore, in this work, for the optimization and scaling up of bacterial nanocellulose, a culture medium consisting of rotten banana unsuitable for human consumption was used for the first time as an inexpensive feedstock. Initially, the bacterial nanocellulose (BNC) culture medium conditions were optimized, and it was established that a glucose concentration of 26.4 g/L and a V/A ratio of 2.2 cm were the optimal conditions for production reaching a BNC yield of 5 g/L, which was 42.4% higher than the best result ini... [more]

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can result in severe damage to the respiratory system. With no specific treatment to date, it is crucial to identify potent inhibitors of SARS-CoV-2 Chymotrypsin-like protease (3CLpro) that could also modulate the enzymes involved in the respiratory damage that accompanies SARS-CoV-2 infection. Here, flavones isolated from Scutellaria baicalensis (baicalein, baicalin, wogonin, norwogonin, and oroxylin A) were studied as possible compounds in the treatment of SARS-CoV-2 and SARS-CoV-2-induced acute lung injuries. We used structural bioinformatics and cheminformatics to (i) identify the critical molecular features of flavones for their binding activity at human and SARS-CoV-2 enzymes; (ii) predict their drug-likeness and lead-likeness features; (iii) calculate their pharmacokinetic profile, with an emphasis on toxicology; (iv) predict their pharmacodynamic profiles, with the identification of their human body targets... [more]

In the present work, a methodology is presented where batch and fixed-bed column tests of dye sorption onto granular biosorbents are analyzed with properly selected models to estimate the parameters required for the rational design of pilot-scale units. The sorption of methylene blue (MB) onto banana peels (BP) was investigated as a case study. To identify the mechanisms of MB sorption onto BP, the pore structure and surface of BP were characterized with mercury intrusion porosimetry (MIP), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Batch tests were performed over the temperature range of 15−45 °C, and three models (Langmuir, Freundlich, Langmuir−Freundlich) were fitted to equilibrium and kinetic data for (i) estimating thermodynamic/kinetic parameters and (ii) choosing the model with the best goodness-of-fit. Sorption tests on fixed-bed columns were combined with a one-dimensional macroscopic convection/dispersion/sorption model to estimate... [more]

Aminoethers of boric acid (AEBA) were studied as potential extractants for the separation of aqueous−alcoholic azeotropic mixtures by extractive distillation. The conditions of vapor−liquid equilibrium in aqueous solutions of ethanol and isopropanol in the presence of AEBA were studied. The division of AEBA molecules into group components was proposed, and previously unknown geometric parameters of the boron group and the energetic pair parameters of the boron group with the alkane group, ether group, amine-3d group, and alcohol group were determined within the framework of the Universal Functional Group Activity Coefficient (UNIFAC) model. The modeling of the extractive rectification process of an ethanol−water mixture with AEBA as extractant has been carried out. The dependences of the cost function on the extractant flow rate, the residual water content in it and the number of theoretical trays were obtained. A technological scheme for ethanol dehydration has been proposed, and its... [more]

This paper describes a design procedure for a collaborative control structure in Plant Wide Control (PWC), taking into account the existing controllable parameters as a novelty in the procedure. The collaborative control structure includes two layers, supervisory and regulatory, which are determined according to the dynamics hierarchy obtained by means of the Hankel matrix. The supervisory layer is determined by the main dynamics of the process and the regulatory layer comprises the secondary dynamics and controllable parameters. The methodology proposed is applied to a wastewater treatment plant, particularly to the Benchmark Simulation Model No 1 (BSM1) for the activated sludge process, comparing the results with the use of a Model Predictive Controller in the supervisory layer. For determining controllable parameters in the BSM1 control, a new specific oxygen mass transfer model in the biological reactor has been developed, separating the kLa volumetric mass transfer coefficient int... [more]

This work describes a novel methodology for the quality assessment of a Fused Filament Fabrication (FFF) 3D printing object during the printing process through AI-based Computer Vision. Specifically, Neural Networks are developed for identifying 3D printing defects during the printing process by analyzing video captured from the process. Defects are likely to occur in 3D printed objects during the printing process, with one of them being stringing; they are mostly correlated to one of the printing parameters or the object’s geometries. The defect stringing can be on a large scale and is usually located in visible parts of the object by a capturing camera. In this case, an AI model (Deep Convolutional Neural Network) was trained on images where the stringing issue is clearly displayed and deployed in a live environment to make detections and predictions on a video camera feed. In this work, we present a methodology for developing and deploying deep neural networks for the recognition of... [more]

This paper provides an overview of nonlinear state estimation techniques along with a discussion on the challenges and opportunities for future work in the field. Emphasis is given on Bayesian methods such as moving horizon estimation (MHE) and extended Kalman filter (EKF). A discussion on Bayesian, deterministic, and hybrid methods is provided and examples of each of these methods are listed. An approach for nonlinear state estimation design is included to guide the selection of the nonlinear estimator by the user/practitioner. Some of the current challenges in the field are discussed involving covariance estimation, uncertainty quantification, time-scale multiplicity, bioprocess monitoring, and online implementation. A case study in which MHE and EKF are applied to a batch reactor system is addressed to highlight the challenges of these technologies in terms of performance and computational time. This case study is followed by some possible opportunities for state estimation in the f... [more]

This study aimed to identify the water-vapor transport mechanisms through an aerated matrix during microwave freeze-drying. Due to the larger surface area and lower water vapor transport resistance of an aerated product compared to the solution, foam structures dry faster. Different foam structures were produced with different maltodextrin (MD) concentrations (10−40%) as a foam-stabilizing agent. Depending on the initial viscosity of the solution prior to foaming, the samples differed in overrun (41−1671%) and pore size (d50 = 58−553 µm). Experiments were partially performed in a freeze-drying chamber of a light microscope to visualize structural changes in-situ. Different mechanisms were identified explaining the accelerated drying of foams, depending on the MD concentration, above or below 30%. At lower MD concentration, high overruns could be produced prior to freezing with big bubbles and thin lamellae with short diffusion pathway length. At 40% MD concentration, the viscosity was... [more]

Supercritical CO2 power cycles have been deeply investigated in recent years. However, their potential in waste heat recovery is still largely unexplored. This paper presents a critical review of engineering background, technical challenges, and current advances of the s-CO2 cycle for waste heat recovery. Firstly, common barriers for the further promotion of waste heat recovery technology are discussed. Afterwards, the technical advantages of the s-CO2 cycle in solving the abovementioned problems are outlined by comparing several state-of-the-art thermodynamic cycles. On this basis, current research results in this field are reviewed for three main applications, namely the fuel cell, internal combustion engine, and gas turbine. For low temperature applications, the transcritical CO2 cycles can compete with other existing technologies, while supercritical CO2 cycles are more attractive for medium- and high temperature sources to replace steam Rankine cycles. Moreover, simple and regener... [more]

Monitoring the evolution of hydrogen gas on carbon steel pipe using acoustic emission (AE) signal can be a part of a reliable technique in the modern structural health-monitoring (SHM) field. However, the extracted AE signal is always mixed up with random extraneous noise depending on the nature of the service structure and experimental environment. The noisy AE signals often mislead the obtaining of the desired features from the signals for SHM and degrade the performance of the monitoring system. Therefore, there is a need for the signal denoising method to improve the quality of the extracted AE signals without degrading the original properties of the signals before using them for any knowledge discovery. This article proposes a non-decimated stationary wavelet transform (ND-SWT) method based on the variable soft threshold function for denoising hydrogen evolution AE signals. The proposed method filters various types of noises from the acquired AE signal and removes them efficiently... [more]

A novel approach to improve ethanol production from sugarcane bagasse is proposed. Biomass was pretreated with sodium hydroxide, sulfuric, oxalic, and maleic acids (1% w/v) at different temperatures (130−170 °C) and times (10−30 min). The pretreatment with NaOH at 160 °C for 20 min was found to be the most efficient for further enzymatic saccharification. A semi-continuous fermentation system coupled with a simultaneous saccharification and fermentation strategy was used, attaining fermented liquor every 24 h. The amount of enzymes needed for saccharification was optimized, as well as the production time and ethanol concentration. The process occurred with near to complete depletion of glucose, obtaining ethanol concentrations ranging from 8.36 to 10.79% (v/v). The whole system, at bench scale, showed stability over 30 days, and ease of management and control. This strategy may improve cost efficiency in the production of cellulosic ethanol at industrial scale.

Microalgae are photoautotrophic microorganisms capable of producing compounds with potential bioenergetic applications as an alternative energy source due to the imminent exhaustion of fossil fuels, their impact on the environment, and the constant population increase. The mass cultivation of these microorganisms requires high concentrations of nutrients, which is not profitable if analytical grade culture media are used. A viable alternative is the use of agro-industrial wastewater, due to the metabolic flexibility of these microorganisms and their ability to take advantage of the nutrients present in these substrates. For the reasons mentioned above, the effect of the cultivation in wastewater from cheese processing on the growth parameters and biomass composition of Scenedesmus sp. was evaluated, and its nutrient removal capacity determined. A high lipid concentration was obtained in the cultures with the dairy effluent (507.81 ± 19.09 mg g−1) compared to the standard culture medium... [more]

The paper presents the comparison of quality of six different commercial apple juices produced in Poland. The apple juices came from two different Polish companies. From each manufacturer three various juices were selected: two cloudy and clarified one produced as a mixture of different apple varieties. The following properties were evaluated: density, soluble solid content, viscosity, total phenolic contents, phenolic acid, and antiradical activity. The obtained results showed that three from fourth cloudy juices (M1Sz, M1A and M2A) differed from clarified juices. The highest differences were noticed for total phenolic contents, phenolic acids, antiradical activity and viscosity. The values of these properties ranged between 60.72−103.6 mg GAE·100−1 mL−1 for phenolic contents, 78.3−90.9% for antiradical activity and 2.68−5.79 mPa·s for viscosity. The remaining cloudy juice (M2Sz) was more similar to the clarified ones. The strong correlation between total phenolic content and antiradi... [more]

The insect cell-baculovirus expression vector system is an established method for large scale recombinant adeno-associated virus (rAAV) production, largely due to its scalability and high volumetric productivities. During rAAV production it is critical to monitor process parameters such as Spodoptera frugiperda (Sf9) cell concentration, infection timing, and cell harvest viabilities since they can have a significant influence on rAAV productivity and product quality. Herein we developed the use of dielectric spectroscopy as a process analytical technology (PAT) tool used to continuously monitor the production of rAAV in 2 L stirred tank bioreactors, achieving enhanced control over the production process. This study resulted in improved manufacturing robustness through continuous monitoring of cell culture parameters, eliminating sampling needs, increasing the accuracy of infection timing, and reliably estimating the time of harvest. To increase the accuracy of baculovirus infection tim... [more]

Artificial construction of spherical protein assemblies has attracted considerable attention due to its potential use in nanocontainers, nanocarriers, and nanoreactors. In this work, we demonstrate a novel strategy to construct peptide nanocapsules (artificial viral capsids) decorated with enzymes via interactions between His-tag and Ni-NTA. A β-annulus peptide derived from the tomato bushy stunt virus was modified with Ni-NTA at the C-terminus, which is directed toward the exterior surface of the artificial viral capsid. The β-annulus peptide bearing Ni-NTA at the C-terminus self-assembled into capsids of about 50 nm in diameter. The Ni-NTA-displayed capsids were complexed with recombinant horseradish peroxidase (HRP) with a C-terminal His-tag which was expressed in Escherichia coli. The β-annulus peptide-HRP complex formed spherical assemblies whose sizes were 30−90 nm, with the ζ-potential revealing that the HRP was decorated on the outer surface of the capsid.

The effect of dried apple varieties on their rehydration characteristics was investigated. Four varieties of apples, Champion, Cortland, Grey Reinette and Ligol, were taken into consideration. Rehydration properties and color of apples were investigated. In order to examine the influence of apple variety on its rehydration properties, the process of rehydration was modeled. The model parameters obtained for investigated apple varieties were compared. Apple cubes were dried in a tunnel dryer (air temperature 60 °C and air velocity 2 m/s) and next rehydrated in distilled water at temperature: 20, 45 and 70 °C. Mass, dry matter mass, volume and color attributes of apples (raw, dried and rehydrated) were measured. The process of rehydration was modeled using empirical (Peleg and Weibull models) and theoretical (the Fick’s second law) models. Results of the analysis showed that the apple variety affects values of mass and volume increase, dry matter decrease and color of the rehydrated appl... [more]

The article deals with the possibilities of using electron accelerator for controlled aging of lubricating media used in special vehicles. During use, e.g., in combustion engines, the lubricants get contaminated and thermo-oxidative degradation also occurs. The pilot project confirms the hypothesis that ionizing radiation makes it possible to simulate the operating load of lubricating media, which was repeatedly confirmed by long-term monitoring of changes in viscosity of statistically significant samples of motor oils used in special equipment. Preliminary test results also show that there are likely to be possibilities to influence other selected properties, such as the coefficient of friction depending on the radiation dose. The authors describe physicochemical processes during irradiation and, in the example of kinematic viscosity, present summary results for selected lubricating media.