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Showing records 38625 to 38649 of 43621. [First] Page: 1 1542 1543 1544 1545 1546 1547 1548 1549 1550 Last
Tertiary Wastewater Treatment Technologies: A Review of Technical, Economic, and Life Cycle Aspects
Dimitris P. Zagklis, Georgios Bampos
February 21, 2023 (v1)
Subject: Environment
Keywords: chlorination, constructed wetlands, life cycle assessment, microalgae, ozonation, technoeconomic, tertiary wastewater treatment
The activated sludge process is the most widespread sewage treatment method. It typically consists of a pretreatment step, followed by a primary settling tank, an aerobic degradation process, and, finally, a secondary settling tank. The secondary effluent is then usually chlorinated and discharged to a water body. Tertiary treatment aims at improving the characteristics of the secondary effluent to facilitate its reuse. In this work, through a literature review of the most prominent tertiary treatment methods, a benchmarking of their technical efficiency, economic feasibility, and environmental impact was carried out. The photo-Fenton method proved to be the most technically efficient process, significantly reducing the microbial load and pharmaceutical content (by 4.9 log and 84%, respectively) of the secondary effluent. Chlorination and UV irradiation exhibited the lowest treatment costs (0.004 EUR/m−3) and the lowest global warming potential (0.04 and 0.09 kg CO2eq. m−3, respectivel... [more]
Research on Parameter Matching of the Asymmetric Pump Potential Energy Recovery System Based on Multi-Core Parallel Optimization Method
Lixin Wei, Zhiqiang Ning, Long Quan, Aihong Wang, Youshan Gao
February 21, 2023 (v1)
Subject: Optimization
Keywords: energy recovery, multi-core CPU, multi-process parallel, serialization products, VAPP
Aiming at the parameters of the different displacements and related components of the variable-displacement asymmetric axial piston pump (VAPP) required by the energy-recovery system of excavator booms of different tonnages, a rapid multi-process parallel optimization method of complex hydraulic products based on a multi-core CPU was proposed for parameter matching. The parameter matching was used to reasonably select relevant parameters so that the excavator’s boom energy-recovery and utilization system can improve operational efficiency and energy-saving efficiency under the premise of satisfying the normal working conditions of the working mechanism, and achieving the purpose of serializing VAPP products. A multi-objective optimization model was put forward according to energy-saving efficiency and operational efficiency. First, the accuracy of the acceleration method of the CVODE, a solver for stiff and non-stiff ordinary differential equation (ODE) systems, was verified by a physi... [more]
Waste Derived Biocomposite for Simultaneous Biosorption of Organic Matter and Nutrients from Green Straw Biorefinery Effluent in Continuous Mode Activated Sludge Systems
Ahmad Hussaini Jagaba, Shamsul Rahman Mohamed Kutty, Lavania Baloo, Gasim Hayder, Abdullahi Haruna Birniwa, Abubakr Taha Bakheit Taha, Mohammed Mnzool, Ibrahim Mohammed Lawal
February 21, 2023 (v1)
Subject: Materials
Keywords: alum sludge, biochar, biocomposite, chemical oxygen demand, green straw biorefinery effluent, mesocarp fiber
The conventional disposal of green straws through burning can be eliminated in a biorefinery that converts them into a range of sustainable commercial products. However, this leads to the generation of green straw biorefinery effluent (GSBE). Green straw biorefineries discharge wastewater into the ecosystem that contains high concentrations of COD and NH4+−N. It is one of the most notable sources of visual pollution and disruption of aquatic life as well as public health that requires treatment prior to discharge. To improve the GSBE quality for environmental sustainability, the attainment of sustainable development goals 6, 9, and 14, “clean water and sanitation”, “inorganic and organic waste utilization for added values from material”, and “life below water” is very important. Therefore, the effectiveness of the continuous mode activated sludge (CMAS) system and the biocomposite-based−continuous mode activated sludge (SB-CMAS) system in the treatment of GSBE was investigated in this... [more]
Deep Learning with Spatial Attention-Based CONV-LSTM for SOC Estimation of Lithium-Ion Batteries
Huixin Tian, Jianhua Chen
February 21, 2023 (v1)
Keywords: deep learning, IOV, lithium-ion battery, SOC
Accurate estimation of the state of charge (SOC) is an indispensable part of a vehicle management system. The accurate estimation of SOC can ensure the safe and reliable operation of the vehicle management system. With the development of intelligent transportation systems (ITS), vehicles can not only obtain the dynamic changes inside the battery through sensors, but also obtain the traffic information around the vehicle through vehicle−road collaboration. In addition, the development of onboard graphic processing units (GPUs) and Internet of Vehicles (IOV) technology make the computing power of vehicles no longer limited by hardware, which makes neural networks applied to the intelligent control of vehicles. Aiming at the problem that the traditional network cannot effectively obtain the complex spatial information of sample attributes, we developed an attention-based CONV-LSTM module for SOC prediction based on a convolutional neural network (CNN) and a long short-term memory (LSTM) n... [more]
Study of Solidifying Surplus Sludge as Building Material Using Ordinary Portland Cement
Jiling Liang, Han He, Jianwei Wei, Tingting Han, Wenwu Wang, Lu Wang, Jie Han, Lunqiu Zhang, Yan Zhang, Haiqiang Ma
February 21, 2023 (v1)
Subject: Materials
Keywords: ordinary Portland cement, solidification, surplus sludge, unconfined compression strength
In an attempt to effectively utilize a multitude of surplus sludge from sewage treatment plants, ordinary Portland cement was used to solidify the dry surplus sludge as a building material. The dry surplus sludge and cement were mixed at different proportions with a certain dosage of water and then cured for 3−60 days at room temperature. The unconfined compression strength (RC) of solidified blocks was investigated with respect to the effects of the ratio of liquid to solid (Rl/S), surplus sludge dosage (DS), the dosage of sodium silicate (DNa2SiO3), and the proportion of fly ash (WF). The fabricated solidified blocks were characterized by scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), and X-ray Diffraction Analysis (XRD). The results demonstrated that RC at 60 days reduced obviously with the increase in Rl/s when Ds was given, whereas RC reduced with DS increased to 15.0 wt% from 5.0 wt% for solidified blocks. When DS was 5.0 wt%, RC of 28 days w... [more]
A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis
Wei-Yang Wang, Shi-Hua Wu, Yuan-Han Xie, Miao Zhong, Man-Li Wei, Ze-Yang Li, Xiu-Feng Long, Fu-Xing Niu
February 21, 2023 (v1)
Keywords: Ethanol, ferric nitrate, pyruvate, Saccharomyces cerevisiae, tolerance engineering
is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, a dual high-throughput screening method of Triphenyl-2H-tetrazoliumchloride (TTC)-based macroscopic observation and the reaction of ferric nitrate with pyruvate (or pyruvate radical ion) in fermentation broth was used. Using this, an S. cerevisiae mutant library that could tolerate 381 g/L sucrose was established by ARTP random mutation and adaptive evolution to select the best strain; its ethanol yield was increased by an additional 20.48%; and the sucrose utilization rate was 81.64%. This method is specific to the selection of strains with increased ethanol production.
Development of a ZrO2-Coating Technique by a Sol−Gel Process Assisted with Pre-Silica-Coating
Akira Miwano, Takehiro Yonezawa, Noriko Yamauchi, Kouichi Nakashima, Yoshio Kobayashi
February 20, 2023 (v1)
Subject: Materials
Keywords: coating, film, silica, sol–gel, zirconia
The development of techniques for coating thermistors with materials possessing excellent chemical resistance and electrical insulation characteristics is necessary to ensure their protection. The present work proposes a coating technique using zirconia (ZrO2), which has excellent chemical resistance and electrical insulation properties, based on initiating a sol−gel reaction of zirconium alkoxide in the presence of a ceramic substrate consisting of the common components of thermistors. The ZrO2 films on substrates were not damaged, even after annealing at 600 °C in air. Several ZrO2 particles were also deposited on the substrate. Pre-silica (SiO2)-coating the substrate, which was performed through a tetraethoxysilane (TEOS) sol−gel reaction, decreased the amount of ZrO2 particles and promoted the formation of thick ZrO2 films, as the reaction between the Zr butoxide and the alkoxyl groups of incompletely hydrolyzed TEOS on the substrate increased the affinity between the ZrO2 layer an... [more]
Dual Input Fuzzy Logic Controllers for Closed Loop Hemorrhagic Shock Resuscitation
David Berard, Saul J. Vega, Guy Avital, Eric J. Snider
February 21, 2023 (v1)
Keywords: closed-loop, control systems, fluid resuscitation, fluid resuscitation, fuzzy logic, hardware-in-loop, hemorrhagic shock
Hemorrhage remains a leading cause of preventable death in emergency situations, including combat casualty care. This is partially due to the high cognitive burden that constantly adjusting fluid resuscitation rates can require, especially in austere or mass casualty situations. Closed-loop control systems have the potential to simplify hemorrhagic shock resuscitation if properly tuned for the application. We have previously compared 4 different controller types using a hardware-in-loop test platform that simulates hemorrhagic shock conditions, and we found that a dual input—(1) error from target and (2) rate of error change—fuzzy logic (DFL) controller performed best. Here, we highlight a range of DFL designs to showcase the tunability the controller can have for different hemorrhage scenarios. Five different controller setups were configured with different membership function logic to create more and less aggressive controller designs. Overall, the results for the different controlle... [more]
Life Cycle Energy Consumption and Greenhouse Gas Emissions Analysis of Primary and Recycled Aluminum in China
Tianduo Peng, Lei Ren, Ershun Du, Xunmin Ou, Xiaoyu Yan
February 21, 2023 (v1)
Subject: Environment
Keywords: energy consumption, greenhouse gas emissions, Life Cycle Analysis, primary and recycled aluminum
Aluminum production is a major energy consumer and important source of greenhouse gas (GHG) emissions globally. Estimation of the energy consumption and GHG emissions caused by aluminum production in China has attracted widespread attention because China produces more than half of the global aluminum. This paper conducted life cycle (LC) energy consumption and GHG emissions analysis of primary and recycled aluminum in China for the year 2020, considering the provincial differences on both the scale of self-generated electricity consumed in primary aluminum production and the generation source of grid electricity. Potentials for energy saving and GHG emissions reductions were also investigated. The results indicate that there are 157,207 MJ of primary fossil energy (PE) consumption and 15,947 kg CO2-eq of GHG emissions per ton of primary aluminum ingot production in China, with the LC GHG emissions as high as 1.5−3.5 times that of developed economies. The LC PE consumption and GHG emiss... [more]
Removal of Hydrophobic Organic Pollutants and Copper by Alginate-Based and Polycaprolactone Materials
Dorinda Marques-da-Silva, João M. Lopes, Iris Correia, João S. Silva, Ricardo Lagoa
February 21, 2023 (v1)
Subject: Materials
Keywords: Adsorption, alginate, anthracene, benzo[a]pyrene, chlorpyrifos, copper, organic matter, organic pollutants, polycaprolactone
Organic pollutants (OPs) and heavy metals are environmental toxicants associated with great concerns. Decontamination processes are urgent for both, and the possibility to achieve their simultaneous removal from polluted waters is highly interesting. Additionally, in many cases, the effect of organic matter in the removal process is overlooked and must be considered. This work aimed to study the potential of alginate-based and polycaprolactone (PCL) materials to remove OPs and copper ions in the absence and presence of organic matter. The OPs investigated were the polycyclic aromatic hydrocarbons anthracene and benzo[a]pyrene, and the pesticide chlorpyrifos, both hydrophobic compounds. Copper (II) ions were used as a model of heavy metals. Alginate-based spheres were prepared by gelation, and PCL microparticles were obtained by oil/water emulsion solvent evaporation. The materials with the highest efficiencies for OP removal from aqueous solutions were those with activated carbon and P... [more]
Decision-Making Model of Mechanical Components in a Lean−Green Manufacturing System Based on Carbon Benefit and Its Application
Xiaoyong Zhu, Yongmao Xiao, Gongwei Xiao
February 21, 2023 (v1)
Subject: Environment
Keywords: carbon benefit, carbon efficiency, carbon emission, lean–green, process decision-making
The key to achieving low-carbon manufacturing is to effectively reduce the carbon emissions of production systems and improve carbon benefits. The use of lean and green tools aids in measuring the added value of products, and increases the efficiency and sustainability of production systems. To address this problem and verify that the synergetic relationship between lean and green innovation increases the efficiency and sustainability in production systems, a new low-carbon manufacturing evaluation indicator—carbon benefit—in lean manufacturing systems was discussed. A low-carbon decision-making model of multiple processes aiming at carbon benefit maximization, as well as the dynamic characteristics of carbon benefit and sustainable process improvements in a lean production system, was established. A case study of a certain satellite dish parts manufacturing line was introduced to analyze and verify the feasibility of the proposed model. After improvement, the processing time of unit p... [more]
Gas Generation and Its Carbon Isotopic Composition during Pyrite-Catalyzed Pyrolysis of Shale with Different Maturities
Yuanhao Cao, Wei Chen, Yinnan Yuan, Tengxi Wang, Jiafeng Sun
February 21, 2023 (v1)
Subject: Materials
Keywords: carbon isotope, pyrite catalysis, shale pyrolysis, thermal maturity
In this study, two shale samples with different maturities, from Geniai, Lithuania (Ro = 0.7%), and Wenjiaba, China (Ro = 2.7%), were selected for open-system pyrolysis experiments at 400 °C and 500 °C, respectively. The generation of isotopic gases from the shales with different maturities was investigated, and the effects of pyrite catalysis on the carbon isotopic compositions were also studied. It was found that CO2, CH4 and their isotopic gases were the main gaseous products of the pyrolysis of both shales, and more hydrocarbon gases were generated from the low-maturity Geniai shale. The δ13C1 values fluctuated from −40‱ to −38‱, and δ13C2 showed higher values (−38‱~−34‱) for the Geniai shale. In addition, its δ13CCO2 values ranged from −28‱ to −26‱. Compared with the Geniai shale, lower δ13C1 values (−43‱~−42‱) and higher δ13CCO2 values (−19‱~−14‱) were detected for the Wenjiaba shale. As temperature increased, CH4 became isotopically lighter and C2H6 became isotopically heavier,... [more]
Deep Learning-Based Human Body Posture Recognition and Tracking for Unmanned Aerial Vehicles
Min-Fan Ricky Lee, Yen-Chun Chen, Cheng-Yo Tsai
February 21, 2023 (v1)
Keywords: activity recognition, deep learning, pose estimation, unmanned aerial vehicles
For many applications (e.g., surveillance and disaster response), situational awareness is essential. In these applications, human body posture recognition in real time plays a crucial role for corresponding response. Traditional posture recognition suffers from accuracy, due to the low robustness against uncertainty. Those uncertainties include variation from the environment (e.g., viewpoint, illumination and occlusion) and the postures (e.g., ambiguous posture and the overlap of multiple people). This paper proposed a drone surveillance system to distinguish human behaviors among violent, normal and help needed based on deep learning approach under the influence of those uncertainties. First, the real-time pose estimation is performed by the OpenPose network, and then the DeepSort algorithm is applied for tracking multi-person. The deep neural network model (YOLO) is trained to recognize each person’s postures based on a single frame of joints obtained from OpenPose. Finally, the fuz... [more]
An Improved Arc Flow Model with Enhanced Bounds for Minimizing the Makespan in Identical Parallel Machine Scheduling
Anis Gharbi, Khaled Bamatraf
February 21, 2023 (v1)
Keywords: identical parallel machines, improved arc flow, integer programming, Scheduling, variable neighborhood search
In this paper, an identical parallel machine problem was considered with the objective of minimizing the makespan. This problem is NP-hard in the strong sense. A mathematical formulation based on an improved arc flow model with enhanced bounds was proposed. A variable neighborhood search algorithm was proposed to obtain an upper bound. Three lower bounds from the literature were utilized in the improved arc flow model to improve the efficiency of the mathematical formulation. In addition, a graph compression technique was proposed to reduce the size of the graph. As a consequence, the improved arc flow model was compared with an arc flow model from the literature. The computational results on benchmark instances showed that the improved arc flow model outperformed the literature arc flow model at finding optimal solutions for 99.97% of the benchmark instances, with the overall percentage of the reduction in time reaching 87%.
Observer-Based Predefined-Time Attitude Control for Spacecraft Subject to Loss of Actuator Effectiveness
Teng Cao, Huajun Gong, Bing Han
February 21, 2023 (v1)
Keywords: attitude control, fault-tolerant control, learning observer, predefined-time stability, spacecraft
The predefined-time tracking problem of spacecraft attitude systems with loss of actuator effectiveness and lumped disturbance including the external disturbance and system uncertainty is studied. In order to obtain the estimation of the actuator efficiency factor more quickly and accurately, a robust learning observer is designed. Based on the fault reconstruction information of the learning observer and the predefined-time stability lemma, a predefined-time tracking fault-tolerant control scheme is proposed for the faulty spacecraft attitude system. The stability of the learning observer and the whole control system is verified by the Lyapunov stability theory. Finally, the effectiveness and advantages of the proposed scheme are illustrated by simulation results including comparisons with existing works.
A New Theobromine-Based EGFRWT and EGFRT790M Inhibitor and Apoptosis Inducer: Design, Semi-Synthesis, Docking, DFT, MD Simulations, and In Vitro Studies
Eslam B. Elkaeed, Reda G. Yousef, Hazem Elkady, Aisha A. Alsfouk, Dalal Z. Husein, Ibrahim M. Ibrahim, Mohamed Alswah, Heba S. A. Elzahabi, Ahmed M. Metwaly, Ibrahim H. Eissa
February 21, 2023 (v1)
Keywords: anti-proliferative, docking, EGFR inhibitors, MD simulation, theobromine
The essential pharmacophoric structural properties were applied to design a new derivative of theobromine as an antiangiogenic EGFR inhibitor. The designed candidate is a (para-nitrophenyl)acetamide derivative of the natural alkaloid, theobromine (T-2-PNPA). The potentialities of T-2-PNPA to inhibit the EGFR protein were studied computationally in an extensive way. Firstly, the molecular docking against EGFRWT and EGFRT790M demonstrated T-2-PNPA’s capabilities of binding with the targeted receptors. Then, the MD experiments (for 100 ns) illustrated through six different studies the changes that occurred in the energy as well as in the structure of EGFR−T-2-PNPA complex. Additionally, an MM-GBSA analysis determined the exact energy of binding and the essential residues. Furthermore, DFT calculations investigated the stability, reactivity, and electrostatic potential of T-2-PNPA. Finally, ADMET and toxicity studies confirmed both the safety as well as the general likeness of T-2-PNPA. Co... [more]
Machine Learning-Based Approach for Modeling the Nanofluid Flow in a Solar Thermal Panel in the Presence of Phase Change Materials
Saeed Alqaed, Jawed Mustafa, Fahad Awjah Almehmadi, Mathkar A. Alharthi, Mohsen Sharifpur, Goshtasp Cheraghian
February 21, 2023 (v1)
Subject: Materials
Keywords: collector, eco-friendly nanoparticles, Machine Learning, PCM, solar energy
Considering the importance of environmental protection and renewable energy resources, particularly solar energy, the present study investigates the temperature control of a solar panel using a nanofluid (NFD) flow with eco-friendly nanoparticles (NPs) and a phase change material (PCM). The PCM was used under the solar panel, and the NFD flowed through pipes within the PCM. A number of straight fins (three fins) were exploited on the pipes, and the output flow temperature, heat transfer (HTR) coefficient, and melted PCM volume fraction were measured for different pipe diameters (D_Pipe) from 4 mm to 8 mm at various time points (from 0 to 100 min). Additionally, with the use of artificial intelligence and machine learning, the best conditions for obtaining the lowest panel temperature and the highest output NFD temperature at the lowest pressure drop have been determined. While the porosity approach was used to model the PCM melt front, a two-phase mixture was used to simulate NFD flow.... [more]
Development of Cement-Based Grouting Material for Reinforcing Narrow Coal Pillars and Engineering Applications
Shunjie Huang, Guangming Zhao, Xiangrui Meng, Xiang Cheng, Qingheng Gu, Gang Liu, Shikui Zhu
February 21, 2023 (v1)
Subject: Materials
Keywords: expansion, grouting material, orthogonal test, roadway support, setting time
The problem needs to be solved about stability control of small coal pillars along goaf in the coal mining process. In this paper, the effects of water reducer, accelerator and expansion agent on the fluidity, setting time, expansion ratio and stone body strength of grouting materials were analyzed through orthogonal experiments, and the optimal ratio of grouting materials was obtained, and it is applied to the engineering site to obtain the reinforcement effect of the coal pillar grouting material on the coal pillar. The results of the study show that: When the dosage of the accelerating agent is 4%, the amount of water-reducer is 0.3%, and the amount of the expansive agent is 6%, the comprehensive performance of the grouting material is the best. After using the new coal pillar grouting material to strengthen the coal pillars, 30 days of monitoring were conducted to determine the deformation of the surrounding rock of the roadway. The shallow separation layer of the surrounding rock... [more]
Challenges and Construction Applications of Solid Waste Management in Middle East Arab Countries
Osama Ibrahim, Ghassan Al-Kindi, Mohsin Usman Qureshi, Salma Al Maghawry
February 21, 2023 (v1)
Subject: Environment
Keywords: Arab countries, construction applications, construction solid waste, health hazards, MENA, municipal solid waste management, Sustainability
Over the past few decades, solid waste production, specifically construction waste, in Middle Eastern Arab countries has dramatically increased. This is characterized by several factors, including rapid urbanization, common food wasting habits, diverse culture, lack of proper planning of solid waste processes, insufficient equipment, as well as lack of proper funding. The exponential growth in solid waste generation rates has led to hazards to health and the environment, causing issues related to air and water pollution under the already increasing pressure of climate change. In this review, we analyze the current solid waste challenges in 13 Arab countries, common diseases, and actual projects applied. The selection of Arab countries was mainly based on the countries with the highest population as well as the availability of data in the field of study. This review also highlights the efforts of the Arab governments that implemented several pilot projects that are not sustainable or ef... [more]
Bioactive Compounds from Elderberry: Extraction, Health Benefits, and Food Applications
Oana-Elena Pascariu, Florentina Israel-Roming
February 21, 2023 (v1)
Keywords: anthocyanins, antioxidants, bioactive compounds, polyphenols, Sambucus nigra
Elderberries are appreciated for their antioxidant properties. Sambucus nigra L. is an extremely abundant plant in the wild flora of Romania, but it is underutilized. Elderberry is used in modern and traditional medicine due to the complex chemical composition of the fruit. The content of phenolic compounds is high (516−8974 mg/100 g DW), of which the most abundant are anthocyanins. Phenolic compounds are known for their beneficial effects on the body. Numerous studies have demonstrated the antioxidant capacity, antibacterial, antiviral, antidiabetic, and anticancer properties of the fruit. It is considered that most of the therapeutic properties of elderberries can be correlated with the antioxidant activity they have. S. nigra fruits are also used in the food industry. Some studies have shown that the therapeutic properties of elderberries can also be found in the products obtained from them. Therefore, this review aimed to describe the chemical composition of elderberries and produc... [more]
Real-Time Temperature and Humidity Measurements during the Short-Range Distribution of Perishable Food Products as a Tool for Supply-Chain Energy Improvements
Martim L. Aguiar, Pedro D. Gaspar, Pedro D. Silva, Luísa C. Domingues, David M. Silva
February 21, 2023 (v1)
Keywords: food preservation, food quality, food waste, humidity, intelligent packaging, Supply Chain, temperature, transportation
Food waste results in an increased need for production to compensate for losses. Increased production is directly related to an increase in the environmental impact of agriculture and in the energy needs associated with it. To reduce food waste, the supply chain should maintain ideal preservation conditions. In horticultural products, temperature, and relative humidity are two of the main parameters to be controlled. Monitoring these parameters can help decision-making in logistics and routes management, as well as to diagnose and timely prevent food losses. In the present work, eighteen wireless traceability devices with temperature and relative humidity sensors monitored crates with horticultural products along a short-range distribution route with five stops (4 h 30 m). Sensor data and a location tag were sent via GSM for real-time monitoring. The results showed fluctuations in temperature and relative humidity that reached up to 7.4 °C and 35.3%, respectively. These fluctuations ha... [more]
Targeted Metabolic Analysis and MFA of Insect Cells Expressing Influenza HA-VLP
Alexandre B. Murad, Marcos Q. Sousa, Ricardo Correia, Inês A. Isidro, Manuel J. T. Carrondo, António Roldão
February 21, 2023 (v1)
Keywords: baculovirus, influenza, insect cells, metabolic flux analysis, VLPs
Virus-like particles (VLPs) are versatile vaccine carriers for conferring broad protection against influenza by enabling high-level display of multiple hemagglutinin (HA) strains within the same particle construct. The insect cell-baculovirus expression vector system (IC-BEVS) is amongst the most suitable platforms for VLP expression; however, productivities vary greatly with particle complexity (i.e., valency) and the HA strain(s) to be expressed. Understanding the metabolic signatures of insect cells producing different HA-VLPs could help dissect the factors contributing to such fluctuations. In this study, the metabolic traces of insect cells during production of HA-VLPs with different valences and comprising HA strains from different groups/subtypes were assessed using targeted metabolic analysis and metabolic flux analysis. A total of 27 different HA-VLP variants were initially expressed, with titers varying from 32 to 512 HA titer/mL. Metabolic analysis of cells during the produc... [more]
Research on the Siting Model of Emergency Centers in a Chemical Industry Park to Prevent the Domino Effect
Kerang Cao, Linqi Liang, Yaru Liu, Liwei Wang, Kwang-Nam Choi, Jingyu Gao
February 21, 2023 (v1)
Subject: Optimization
Keywords: chemical industry park, emergency siting, multi-objective optimization, NSGA-II
A chemical industry park (CIP) has a wide variety of hazardous chemicals, and once an accident occurs, the level of danger increases geometrically, while the domino effect may bring devastating consequences. To improve the emergency rescue capability of a chemical park and prevent the domino effect, a certain number of emergency centers are built at sites near the park for the purpose of rapid emergency rescue and deployment of emergency supplies. Based on this, in our study, a siting model of the emergency center of the chemical park, which aims to prevent the domino effect, was constructed by considering the timeliness and safety, while adopting the prevention of the domino effect as a constraint. The NSGA-II algorithm is used to solve the siting model, and the CPLEX method is used for the comparison. This study combines the prevention of the domino effect with multi-objective optimization theory, which has a good and simple applicability for solving the considered problem and can ob... [more]
Providing Antimicrobial Properties to Cardboard Food Packaging by Coating with ZnO, TiO2, and SiO2—Water-Based Varnish Nanocomposites
Tomislav Hudika, Nevijo Zdolec, Marta Kiš, Tomislav Cigula
February 21, 2023 (v1)
Keywords: antimicrobial activity, cardboard packaging, nanocomposite coatings, surface free energy
Packaging acts like a bond between visual communication and production technology. Packaging material is often coated to enhance visual appearance and some protective features. The COVID pandemic changed consumers’ behavior and understanding of the importance regarding the antimicrobial properties of goods that come in contact with hands. The aim of this research is to investigate and determine the antimicrobial properties of nanocomposite coatings which include nanosized zinc oxide (ZnO), titanium dioxide (TiO2), and silicon dioxide (SiO2). For the purpose of this research, a lithographic printed packaging was coated with a nanocomposite composed of flexographic water-based varnish with incorporated ZnO, TiO2, and SiO2 nanosized particles. A total of eight modulations were presented and compared to the lone water-based varnish. The results have shown that applying nanocomposites will increase the total surface free energy of the packaging surface but will decrease the polar component... [more]
An Analysis of Energy and Internal Flow Characteristics of Open Inlet Channel Axial Flow Pumping Devices
Chuanliu Xie, Cheng Zhang, Tenglong Fu, Andong Feng, Tao Zhang, Fan Yang
February 21, 2023 (v1)
Keywords: axial flow pump device, energy characteristics, internal flow characteristics, numerical calculation, open inlet channel
For the purpose of studying the dynamic and inner flow features of an open inlet channel axial flow pump unit, in the present study, numerical calculations using the SST k-ω turbulence model are applied to an open inlet channel axial flow pumping unit based on the NS equation, and experimental validation is then performed. The experimental output indicates that the designed working conditions are Q = 350 L/s, head H = 5.065 m, efficiency η = 79.56%, and the maximum operating head is H = 9.027 m, which is about 1.78 times that of the design head; further, the pump device can operate in a wide range of working conditions. In addition, the design working conditions are within the range of high-efficiency operating conditions. The calculated values and the experimental comparison are all within a 5.0% margin of error; further, the numerical calculations are reliable. The hydraulic loss of the inlet channel under the design condition Q = 350 L/s is 0.0676 m, which satisfies the relationship... [more]
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