A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

Wei-Yang Wang; Shi-Hua Wu; Yuan-Han Xie; Miao Zhong; Man-Li Wei; Ze-Yang Li; Xiu-Feng Long; Fu-Xing Niu

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2023.0742

Published Article

LAPSE:2023.0742

A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

Wei-Yang Wang, Shi-Hua Wu, Yuan-Han Xie, Miao Zhong, Man-Li Wei, Ze-Yang Li, Xiu-Feng Long, Fu-Xing Niu

February 21, 2023

is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, a dual high-throughput screening method of Triphenyl-2H-tetrazoliumchloride (TTC)-based macroscopic observation and the reaction of ferric nitrate with pyruvate (or pyruvate radical ion) in fermentation broth was used. Using this, an S. cerevisiae mutant library that could tolerate 381 g/L sucrose was established by ARTP random mutation and adaptive evolution to select the best strain; its ethanol yield was increased by an additional 20.48%; and the sucrose utilization rate was 81.64%. This method is specific to the selection of strains with increased ethanol production.

Record ID

LAPSE:2023.0742

Keywords

Ethanol, ferric nitrate, pyruvate, Saccharomyces cerevisiae, tolerance engineering

Subject

Energy Systems

Suggested Citation

Wang WY, Wu SH, Xie YH, Zhong M, Wei ML, Li ZY, Long XF, Niu FX. A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis. (2023). LAPSE:2023.0742

Author Affiliations

Wang WY: Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China
Wu SH: Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China
Xie YH: Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China
Zhong M: Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China
Wei ML: Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China
Li ZY: Key Laboratory for Zhuang and Yao Pharmaceutical Quality Biology, Guangxi Science & Technology Normal University, Laibin 546199, China
Long XF: Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China
Niu FX: Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China [ORCID]

Journal Name

Processes

Volume

10

Issue

11

First Page

2186

Year

2022

Publication Date

2022-10-25