Chemical investigations of L. stoechas subsp. luisieri and L. pedunculata essential oils were analyzed by GC-MS, and the antimicrobial activity was performed against bacteria and fungi isolated from food sources. The cytotoxicity of the essential oil was performed in NHDF cells using the MTT method. According to the results, the main compounds of L. stoechas subsp. luisieri essential oil were trans-α-necrodyl acetate (40.2%), lavandulyl acetate (11%), and trans-α-necrodol (10.4%), while fenchone (50.5%) and camphor (30.0%) in L. pedunculata essential oil. The antifungal activity of essential oils was confirmed with MIC values ranging from 1.2 to 18.7 µL/mL; for bacteria, it ranged from 4.7 to 149.3 µL/mL. Both the Lavandula species tested showed low or equal MIC and MBC/MFC values for L. stoechas subsp. luisieri essential oil, revealing greater efficacy in antimicrobial activity. The L. stoechas subsp. luisieri essential oil revealed cytotoxic effects (30 ± 2% of cell viability) in NHD... [more]

Soaking is an important step in making tempeh. Tempeh fermentation normally involves the natural presence of proteolytic bacteria capable of producing protease enzymes to break down peptide bonds in protein molecules. This study evaluated the protein and amino acid content of Jack bean tempeh (Canavalia ensiformis) soaked in distilled water during natural fermentation for 12, 24, 36, and 48 h. In this study, the crude proteins were determined using the Kjeldahl technique, amino acids were determined from protein hydrolysis, and proteolytic bacteria were enumerated for Total Plate Counts and further identified using Vitek 2.0 Compact System. The results showed that soaked Jack beans have higher protein and amino acid content, with sixteen essential and non-essential amino acids required for human bodies. The protein content of soaked Jack beans varied from 35% at 12 h to 32% at 24 and 36 h and 33% at 48 h. Soaking for 12 h yielded the highest amino acid concentration of 38,000 mg/kg L-g... [more]

A green method for hydroxypropyl-β-cyclodextrin-glycerol-assisted extraction (HCGAE) of bioactive phenolics from S. montana, Lamiaceae was optimized using Box-Behnken design and response surface methodology and compared conventional water/ethanol-based extraction. The procedure was aimed at obtaining extracts with the maximum content of total phenols (TP), flavonoids (TF), dihydroxycinnamic acids (TDCA), rosmarinic acid (RA), and luteolin 7-O-glucoside (LG). The impact of glycerol content (0−70%), 2-hydroxypropyl-β-cyclodextrin content (0−0.4 mmol), temperature (20−70 °C), herbal material weight (0.3−0.8 g), extraction duration (5−25 min), and ultrasound power (144−720 W) on the extraction efficiency was analyzed. Four extracts with maximum amounts of target phenols, OPT-TP (5.93 mg/mL), OPT-TDCA-RA (4.17 mg/mL and 1.16 mg/mL, respectively), OPT-TF (0.99 mg/mL), and OPT-LG (0.28 mg/mL) were prepared. Comparison of the content of TDCA, TF, RA, and LG with those obtained in water/ethanol... [more]

Colorectal cancer (CRC) is one of the most common cancers in the world. When treating patients, therapeutic agents have side effects; hence, the use of natural compounds found in medicinal plants including pomegranate. Ultrasound assisted extraction (UAE) is a new technique evolving to the detriment of traditional methods such as maceration. In this study, we investigated the antioxidant and anticancer effect of pomegranate peel extracts obtained by maceration and UAE at three different ultrasonic power levels (P1 = 10 W; P2 = 50 W; P3 = 100 W) on HCT-116 colorectal cancer cells. Phytochemical screening highlighted the presence of primary and secondary metabolites in pomegranate peels. In addition, the ethanolic extract obtained by UAE at 50 W was shown to be the most concentrated in phenolic and flavonoid compounds and have the most powerful antioxidant activity, which reached a maximum activity of 92% as determined by DPPH test. Similarly, the MTT cell viability test showed that the... [more]

The objective of this study was to evaluate the effects of different types of powder additions on the properties of corn extrudates. The following ingredients, which are good sources of bioactive compounds, were used to substitute corn flour: legume protein sources (2% pea, 5% broccoli, and 5% lucerne), plants (15% beetroot and 15% rosehip), and condiments (2% chili, 2% turmeric, 2% paprika, and 2% basil). The total polyphenolic content (TPC) and antioxidant activity (AA) increased when the corn flour was replaced with the different types of ingredients. The highest TPC was found for rosehip followed by the beet, basil, and broccoli additions. Compared to the raw formulations, all the extrudates, except the rosehip extrudate, showed a decrease in the TPC ranging from 11 to 41%, with the smallest loss (11%) occurring for basil and the highest loss (41%) occurring for the control extrudate, respectively. The same observation was recorded for the AA. For the extrudate enriched with rosehi... [more]

One of the most advanced systems of microbial fuel cells is the benthic microbial fuel cell (BMFC). Despite several developments, this strategy still has a number of significant flaws, such as instable organic substrate. Waste material (sugarcane) is used as a substrate in this work to address the organic substrate instability. The process was operated continuously for 70 days. A level of 300 mV was achieved after 33 days of operation, while the degradation efficiencies of Pb (II), Cd (II), and Cr (III) were more than 90%. More than 90% of the removed chemical oxygen demand (COD) was also recorded. The measured power density was 3.571 mW/m2 at 1000 external resistance with 458 internal resistance. This demonstrates that electrons are effectively transported throughout the operation. The Bacillus strains are the most dominant bacterial community on the surface of the anode. This research’s mechanism, which involves metal ion degradation, is also explained. Finally, parameter optimizatio... [more]

Accurate polyp segmentation is of great importance for the diagnosis and treatment of colon cancer. Convolutional neural networks (CNNs) have made significant strides in the processing of medical images in recent years. The limited structure of convolutional operations prevents CNNs from learning adequately about global and long-range semantic information interactions, despite the remarkable performance they have attained. Therefore, the GCCSwin-UNet framework is suggested in this study. Specifically, the model utilizes an encoder−decoder structure, using the patch-embedding layer for feature downsampling and the CSwin Transformer block as the encoder for contextual feature extraction. To restore the feature map’s spatial resolution during upsampling operations, a symmetric decoder and patch expansion layer are also created. In order to help the backbone module to do better feature learning, we also create a global context module (GCM) and a local position-enhanced module (LPEM). We co... [more]

Significant efforts have been made toward developing sustainable reduction reactions of organic and bioorganic compounds. In these studies, the selection of reagents and solvents has played a very important role in the development of environment-friendly methodologies. In this context, the reducing agent Cp2TiCl/H2O has been introduced as a safe, efficient, selective, and low-cost reagent, and thus as a sustainable alternative for the reduction of organic compounds. To facilitate understanding of the reductions mediated by this system, in this study we focus on describing the intermediates, mechanisms, and representative examples. Finally, a reflection is made on the future perspectives of this reducing agent, including its analog Cp2TiCl/D2O as a powerful tool for the preparation of deuterated phenols, which can be successfully used as an internal standard for analyzing bioactive phenols in olive oil.

is a halophyte indigenous to marine coastal areas and salt fields and has been used as a traditional remedy for diarrhea, abdominal pain, constipation, and indigestion. Its component isorhamnetin-3-O-glucoside (IR3G) may have antioxidant, anti-inflammatory, and anti-adipogenic properties. In the present study, we aimed to investigate the anti-obesity effect of S. herbacea extract and IR3G on mouse 3T3-L1 adipocytes and db/db obesity mice. S. herbacea extract and IR3G inhibited lipase in a concentration-dependent manner. Oil Red O staining disclosed that S. herbacea extract and IR3G significantly suppressed lipid accumulation and adipogenesis and also inhibited the expression of the C/EBPα in the 3T3-L1 adipocytes. In experiments using db/db mice, administering of S. herbacea extract limited body weight gain and significantly reduced feed efficiency and adipose tissue weight. Moreover, analyzing blood triglycerides, total cholesterol, high-density lipoprotein, and low-density lipoprotei... [more]

Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production a... [more]

The dewatering process reduces the water quantity in sludge, allowing the decrease in its volume, which facilitates its storage, transport, stabilization, and improves the post-treatment efficiency. Chemical polymers including aluminum sulphate and polyaluminum chloride were applied as flocculants in the conditioning process in order to prepare sludge for dewatering. However, these synthetic polymers may cause risks for human health, and should be substituted with ecofriendly and safe materials. These materials include plant-based flocculants, animal-based flocculants, and microbial-based flocculants. Sludge dewaterability was evaluated by considering many parameters, such as moisture content (MC), dry solids (DS), specific resistance to filtration (SRF), capillary suction time (CST), and sludge volume index (SVI). The use of microorganisms for sludge dewatering is an available option, since many strains (R. erythropolis, A. ferrooxidans, P. mirabilis, T. flavus, etc.) demonstrated the... [more]

The existence of a growing myopia pandemic is an unquestionable fact for health authorities around the world. Different possible causes have been put forward over the years, such as a possible genetic origin, the current excess of children’s close-up work compared to previous stages in history, insufficient natural light, or a multifactorial cause. Scientists are looking for different possible solutions to alleviate it, such as a reduction of time or a greater distance for children’s work, the use of drugs, optometric correction methods, surgical procedures, and spending more time outdoors. There is a growing number of articles suggesting insufficient natural light as a possible cause of the increasing levels of childhood myopia around the globe. Technological progress in the world of lighting is making it possible to have more monochromatic LED emission peaks, and because of this, it is possible to create spectral distributions of visible light that increasingly resemble natural light... [more]

Due to the spread of the SARS-CoV-2 virus, most countries have tightened their public health policies. One way to limit the spread of the virus is to make mouth and nose cover compulsory in public spaces. The article presents the impact of wearing masks on the perception of thermal comfort. The following masks were analysed: FFP2, cotton, medical, PM2.5, half-face protective shield plastic and full-face protective shield plastic. The research was carried out for two scenarios of an ambient temperature: −20 and 30 °C. A thermal manikin was used for the tests. In the case of when a temperature equals 20 °C, the dry masks increase comfort, both general and local, while wet masks reduce comfort. On the other hand, at 30 °C, only wet masks do not increase discomfort. In addition, moist masks require less heat flux to achieve a certain skin temperature. However, it should be remembered that it is not advisable to wet the masks from the health point of view.

Heavy metals have adverse effects on microalgae metabolism and growth. Photosynthesis and lipid profile are quite sensitive to heavy metal toxicity. The impact of hexavalent chromium—Cr(VI) on photosynthesis and lipid accumulation in Mucidosphaerium pulchellum and Micractinium pusillum exposed to different concentrations (0−500 μg L−1) was investigated for 11 days. A significant (p < 0.05) increase in lipid content was observed with increasing Cr(VI) concentration. However, growth was suppressed at higher concentrations exceeding 100 μg L−1. Addition of Cr(VI) in the cell culture medium showed a negative effect on quantum yield (Fv/Fm), and a photosynthetic inhibition of >65% was noted in both species at 500 μg L−1. However, the lipid gravimetric analysis presented inner cell lipid content up to 36% and 30% of dry weight biomass for M. pulchellum and M. pusillum, respectively. The fatty acids profiles of both microalgae species showed higher levels of hexadecenoic acid as well as ω3... [more]

In recent years, the discovery of two gas fields in the fourth member of the Leikoupo Formation in the Western Sichuan Basin of SW China confirmed the exploration potential of microbial carbonates. The aim of the present study is to clarify the formation mechanism of the microbial reservoirs in the Leikoupo Formation. For this purpose, lithofacies, depositional environments, and diagenesis analyses were performed in samples collected from cores of 12 wells. The climate of study area was arid during Anisian time, and the water body was restricted. In such a climate, an evaporitic environment was developed, where ten types of lithofacies, dominated by microbial carbonates and gypsum rocks, were recognized. Thrombolites and stromatolites are the main high-quality reservoirs rock types in the fourth member of the Leikoupo Formation in the Western Sichuan Basin of SW China, which developed as microbial mounds, with reservoir space of microbial inter-clot pores, intra-clot pores, fenestral p... [more]

The variation of substrate concentration in anode chamber directly affects the power generation efficiency and decontamination performance of microbial fuel cell (MFC). In this study, three concentrations of swine wastewater with 800 mg/L, 1600 mg/L and 2500 mg/L were selected as substrates, and the performance of MFC and response characteristics of anode microbial community were investigated. The results show that the concentration of a selected substrate is positively correlated with the output voltage of MFC and chemical oxygen demand (COD) removal rate. The microbial community diversity in the anode chamber and the performance of battery can be significantly affected when concentration changes in different ways, which helps to selectively cultivate the adaptable dominant bacteria to enhance the stability and decontamination performance of MFC. The community structure of anodic biofilm is mainly composed of Proteobacteria, Bacteroidetes, Firmicutes, Chloroflexi and Spirochaetae. The... [more]

The exposure to chemicals in detergent factories, as some studies have shown, has a negative impact the health of workers’ respiratory systems. The aim of the study was to assess the lung function parameters of detergent factory workers and compare it with those of a group of non-chemical workers using spirometry. Methods: A comparative cross-sectional study was performed involving male detergent factory workers and a comparison group of non-chemical workers. An interviewer-administered questionnaire was used to record workers’ characteristics, and the data were entered to the spirometry device. The spirometry test was performed to assess workers’ lung functions; we recorded the forced expiratory volume in the first second (FEV1), forced vital capacity (FVC), FEV1/FVC ratio, peak expiratory flow (PEF), forced expiratory flow between 25 and 75% of FVC (FEF25−75%), and lung age. Results: A total of 94 participants (28 detergent factory workers and 66 non-chemical workers) were included i... [more]

In the present study, the electrochemical behavior of antiviral drug umifenovir (Umi) and umifenovir encapsulated in phospholipids micelles (nanosome/umifenovir, NUmi) were investigated for the first time on screen-printed electrodes modified by carbon nanotubes. We have shown that Umi can be electro oxidized around the potential of +0.4 V in the concentration range of 50−500 µM (R2 = 0.992). Non-overlapping signatures of DNA and umifenovir (10−150 µM) permit to register interaction between umifenovir (or umifenovir encapsulated in phospholipids micelles), purine, and pyrimidine heterocyclic bases of DNA separately. The type of interaction is most likely via electrostatic interactions and groove binding in drug-DNA formed complex, as was revealed based on the values of binding constants Kb and the cathodic shifts of oxidation potentials for heterocyclic bases with increasing Umi or NUmi concentration. The negative values of Gibbs free energy (ΔG) for all nucleobases confirm the process... [more]

Signaling molecules act as the links and bridges between endophytes and host plants. The recognition of endophytes and host plants, the regulation of host plant growth and development, and the synthesis of secondary metabolites are not separated by the participation of signaling molecules. In this review, we summarized the types and characteristics of signaling molecules in medicinal plants and the recent processes in intracellular conduction and multi-molecular crosstalk of signaling molecules during interactions between endophytic bacteria and medicinal plants. In addition, we overviewed the molecular mechanism of signals in medical metabolite accumulation and regulation. This work provides a reference for using endophytic bacteria and medicinal plants to synthesize pharmaceutical active ingredients in a bioreactor.

Wound healing is the process of skin and soft tissue repair following an injury. Angiogenesis is an essential process in wound healing and plays an important role in tissue regeneration. Ginseng is mainly composed of saponins and protopanaxadiol-based ginsenosides, namely Rb1, Rb2, Rc, Re, Rg1, and Rf. 20(S)-protopanaxadiol (PPD) and 20(S)-protopanaxatriol (PPT) are aglycones of ginsenosides produced by metabolic processes and heat treatment. This study aimed to investigate the wound healing effects of active ingredients of ginseng, namely ginsenosides and aglycones, in various cellular and animal skin wound models. The angiogenic effects of ginsenosides were investigated in human umbilical vein endothelial cells (HUVECs). All experiments were conducted at increased intracellular glucose concentrations and the induction of angiogenesis through tube formation was evaluated. Among the ginsenosides and aglycones used in this study, PPD showed the strongest wound-healing activity. Cell scr... [more]

Microbial fuel cells (MFCs) have recently attracted more attention in the context of sustainable energy production. They can be considered as a future solution for the treatment of organic wastes and the production of bioelectricity. However, the low output voltage and the low produced electricity limit their applications as energy supply systems. The scaling up of MFCs both by developing bigger reactors with multiple electrodes and by connecting several cells in stacked configurations is a valid solution for improving these performances. In this paper, the scaling up of a single air-cathode microbial fuel cell with an internal volume of 28 mL, has been studied to estimate how its performance can be improved (1523 mW/m3, at 0.139 mA). Four stacked configurations and a multi-electrode unit have been designed, developed, and tested. The stacked MFCs consist of 4 reactors (28 mL × 4) that are connected in series, parallel, series/parallel, and parallel/series modes. The multi-electrode un... [more]

Gelatin production is the most industry polluting process where huge amounts of raw organic materials and chemicals (HCl, NaOH, Ca2+) are utilized in the manufacturing accompanied by voluminous quantities of end-pipe effluent. The gelatinous wastewater (GWW) contains a large fraction of protein and lipids with biodegradability (BOD/COD ratio) exceeding 0.6. Thus, it represents a promising low-cost substrate for the generation of biofuels, i.e., H2 and CH4, by the anaerobic digestion process. This review comprehensively describes the anaerobic technologies employed for simultaneous treatment and energy recovery from GWW. The emphasis was afforded on factors affecting the biofuels productivity from anaerobic digestion of GWW, i.e., protein concentration, organic loading rate (OLR), hydraulic retention time (HRT), the substrate to inoculum (S0/X0) ratio, type of mixed culture anaerobes, carbohydrates concentration, volatile fatty acids (VFAs), ammonia and alkalinity/VFA ratio, and reactor... [more]

Hydrogen is currently receiving significant attention and investment as a key enabler of defossilised global energy systems. Many believe this will eventually result in the international trade of hydrogen as a commodity from countries with significant renewable energy resources, for example New Zealand and Australia, to net energy importing countries including Japan and Korea. Japan has, since 2014, been actively exploring the components of the necessary supply chains, including the assessment of different hydrogen carriers. Public/private partnerships have invested in demonstration projects to assess the comparative merits of liquid hydrogen, ammonia, and organic carriers. On the supply side, significant projects have been proposed in Australia while the impending closure of New Zealand’s Tiwai Point aluminium smelter at the end of 2024 may provide an opportunity for green hydrogen production. However, it is also evident that the transition to a hydrogen economy will take some years a... [more]

In this study, the links between performance and microbial communities were investigated with municipal solid waste (MSW) at two feedstock ratios and eight organic loading rates (OLRs). The co-digestion systems were stably operated at OLRs of 2.0−6.0 g VS/(L·d). The performance of the co-digestion system varied with the feedstock ratio. Compared with the 50:50 (hybrid Pennisetum:MSW) system, the 75:50 system, GM31, obtained increases of 1.93−17.68% and 0.29−23.29% for the specific biogas and methane yields, respectively. Whereas a shift in bacterial and methanogen communities occurred as the operating conditions changed, particularly with OLR variations. The genera Saccharofermentans, Prevotella, Clostridium, Syntrophomonas, and Proteiniphilum became the dominant bacteria for the conversion of carbohydrates and nitrogen compounds as the OLR increased. Meanwhile, a shift from acetoclastic to hydrogenotrophic or multifunctional methanogens was observed.

The management and optimization of the aqueous phase are the major challenges that hinder the promotion of hydrothermal liquefaction (HTL) technology on a commercial scale. Recently, many studies reported about the accumulation of the N-content in the bio-crude with continuous recycling of the aqueous phase from high protein-containing biomass. In the present study, sewage sludge was processed at 350 °C in an autoclave. The produced aqueous phase was treated with activated carbon, and its subsequent recycling effect on the properties of the bio-crude and aqueous phase was investigated. By contacting the aqueous phase with activated carbon, 38−43% of the total nitrogen was removed from the aqueous phase. After applying the treated aqueous phase recycling, the energy recovery of the bio-crude increased from 50 to 61% after three rounds of recycling. From overall carbon/nitrogen recoveries, 50 to 56% of the carbon was transferred to the bio-crude phase and more than 50% of the nitrogen re... [more]