Butyl acetate (BuAc) is widely used as a solvent in many applications, mainly in the food and pharmaceutical industries. The conventional process for BuAc production is both capital and energy intensive. The purification process involves the separation of BuAc from the azeotropic mixture of water and n-butanol, which is difficult to accomplish using a simple distillation unit. In this study, a membrane reactor (MR) for BuAc production via the esterification of n-butanol was investigated. The MR using the Amberlyst-15 catalyst was modeled and validated with previously reported experimental data, and a good agreement was achieved. The ultimate conversion of n-butanol using the MR was 92.0%, compared to 69.8% for the conventional reactor. This study is the first to propose an intensified MR-based process for butyl acetate production. The MR-based process was developed and rigorously simulated using Aspen Plus for an annual plant capacity of 92,500 metric tons of BuAc. The MR-based process... [more]

In this article, we present the design and synthesis of novel compounds, containing in their molecules an L-valine residue and a 4-[(4-bromophenyl)sulfonyl]phenyl moiety, which belong to N-acyl-α-amino acids, 4H-1,3-oxazol-5-ones, 2-acylamino ketones, and 1,3-oxazoles chemotypes. The synthesized compounds were characterized through elemental analysis, MS, NMR, UV/VIS, and FTIR spectroscopic techniques, the data obtained being in accordance with the assigned structures. Their purities were verified by reversed-phase HPLC. The new compounds were tested for antimicrobial action against bacterial and fungal strains, for antioxidant activity by DPPH, ABTS, and ferric reducing power assays, and for toxicity on freshwater cladoceran Daphnia magna Straus. Furthermore, in silico studies were performed concerning the potential antimicrobial effect and toxicity. The results of antimicrobial activity, antioxidant effect, and toxicity assays, as well as of in silico analysis revealed a promising po... [more]

This paper presents a descriptor representation-based guaranteed cost design methodology for polynomial fuzzy systems. This methodology applies the descriptor representation for presenting the closed-loop system of the polynomial fuzzy model with a parallel distributed compensation (PDC) based fuzzy controller. By the utility of descriptor representation, the guaranteed cost control (GCC) design analysis can utilize polynomial fuzzy slack matrices for obtaining less conservative results. The proposed GCC design is presented as the sum-of-squares (SOS) conditions. The application of polynomial fuzzy slack matrices leads to the double fuzzy summation issue in the control design. Accordingly, the copositive relaxation works out the problem well and is adopted in the control design analysis. The GCC design minimizes the upper limit of a predesignated cost function. According to the performance function, two simulation examples are provided to demonstrate the validity of the proposed GCC de... [more]

The mechanical characterization of adipose tissues is important for various medical purposes, including plastic surgery and biomechanical applications, such as computational human body models for the simulation of surgical procedures or injury prediction, for example, in the evaluation of vehicle crashworthiness. In this context, the measurement of human subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) mechanical properties in relation to subject characteristics may be really relevant. The aim of this work was to properly characterize the mechanical response of adipose tissues in patients with obesity. Then, the data were exploited to develop a reliable finite element model of the adipose tissues characterized by a constitutive material model that accounted for nonlinear elasticity and time dependence. Mechanical tests have been performed on both SAT and VAT specimens, which have been harvested from patients with severe obesity during standard laparoscopic sleeve gas... [more]

is an edible mushroom growing in nutritionally poor and desert soil. There has been little information available about its edaphic and culturing conditions for achieving the vigorous mycelial growth essential for its artificial cultivation, bioaugmentation and biodegradation in unfertile soil. Thus, the present study was conducted to assess its edaphic conditions and find a suitable culturing medium for obtaining maximum growth. It grows commonly in coastal soil with saline conditions, barren land soil unfit for cultivation, and desert soil. It forms a basidiocarp singly around xerophytic trees and annual plants and also in soil without vegetation. In addition to a well-developed pileus and stipe, it has a typical rhizoid that grows horizontally in soil. The rhizoid was thick at the base of the stipe and became thin into the mycelial strand. In our earlier study, we reported that its mycelial growth was very poor on nutrient-rich media containing simple sugar, for example, glucose. In... [more]

In recent years, monoclonal antibodies (mAbs) are gaining a wide market share as the most impactful bioproducts. The development of mAbs requires extensive experimental campaigns which may last several years and cost billions of dollars. Following the paradigm of Industry 4.0 digitalization, data-driven methodologies are now used to accelerate the development of new biopharmaceutical products. For instance, predictive models can be built to forecast the productivity of the cell lines in the culture in such a way as to anticipate the identification of the cell lines to be progressed in the scale-up exercise. However, the number of experiments that can be performed decreases dramatically as the process scale increases, due to the resources required for each experimental run. This limits the availability of experimental data and, accordingly, the applicability of data-driven methodologies to support the process development. To address this issue in this work we propose the use of digital... [more]

Exploring new solvents for efficient acid gas removal is one of the most attractive topics in industrial gas purification. Herein, using 2-tertiarybutylamino-2-ethoxyethanol as an absorbent in a packed column at atmospheric pressure was examined for selective absorption of H2S from mixed gas streams. In the present work, the acid gas load, H2S absorption selectivity, acid gas removal ratio, amine solution regeneration performance, and corrosion performance were investigated through evaluating experiments absorbing H2S and CO2 by using methyldiethanolamine and 2-tertiarybutylamino-2-ethoxyethanol. The experimental results illustrate that the H2S absorption selective factors were 3.88 and 15.81 by using 40% methyldiethanolamine and 40% 2-tertiarybutylamino-2-ethoxyethanol at 40 °C, respectively, showing that 2-tertiarybutylamino-2-ethoxyethanol is an efficient solvent for selective H2S removal, even better than methyldiethanolamine. Based on the consideration of cost, we added 5% TBEE to... [more]

With the gradual liberalization of China’s energy market, the distributed characteristics of each entity in the community integrated energy system are more and more obvious, and the traditional centralized optimization is difficult to reveal the interaction between the entities. This paper aims to improve the profit of the community operator and the users’ value-added benefit of energy use, and proposes a multi-energy transaction decision of a community integrated energy system considering user interaction. First, a refined model of user interaction, including energy conversion, is established, and then the optimization model of multi-energy transaction decision between the community operator and the users is constructed based on the master−slave game. The upper layer aims to maximize the profit of the community operator according to the energy use strategies’ feedback from the users, decides the retail energy prices of the community operator to the users, and optimization variables in... [more]

This study aimed to evaluate different parameters in the green process of organic Spirulina biomass (SB) C-phycocyanin (C-PC) extraction to understand the impact on weight and oral glucose tolerance of C-PC extract in Swiss mice fed with a high-fat diet (HFD). The proximate composition and antioxidant activity were analyzed in Spirulina by-products: SB, C-PC, and Remaining biomass (RB). The protein content on a dry basis was 52.05% in SB and 61.16% in RB and 118.97 μg/g in C-PC. The antioxidant activity was equal for SB and C-PC but higher than RB. However, RB can be considered a promising ingredient, promoting the sustainable use of the whole SB. Swiss mice were distributed in five groups: control diet (CD), HFD, HFD plus Spirulina biomass (HFDS), HFD plus C-PC (HFDC), and HFD plus remaining biomass (HFDR). HFDS increased the delta weight of the animals and showed glucose intolerance compared to the CD and HFDC groups. The results demonstrated that the supplementation of 500 mg/kg of... [more]

Carbon-reinforced concrete (CRC) has the potential to play a pivotal role in optimizing the built environment and has therefore been experiencing a wave of research and development in the construction industry in recent years. The production of carbon fibers for CRC is energy-intensive, prompting the need to explore circular economy approaches (e.g., recycling at the End-of-Life (EoL)) to optimize the environmental performance of this material. Underdeveloped processes and a resulting lack of primary data regarding the recycling of CRC have hampered a comprehensive sustainability assessment of the novel composite building material. The novelty of this article is the detailed presentation of possible EoL scenarios for CRC and the detailed determination of the respective environmental impacts. This study aims to model EoL options within a Life Cycle Assessment (LCA), focusing on the EoL stage based on ISO 14040/44 using the GaBi ts 10.5.1.124 software and the CML2001 (2016) methodology.... [more]

Extractive distillation with salts, unlike other dehydration technologies, is better due to the null toxicity that exists in the distillate, since salt cannot be evaporated. With this distillation technology, it is possible to obtain a high concentration of ethanol, however, there are still problems in the control of the distillation columns in the presence of disturbances. The present work deals with the simulation and control of an extractive distillation column using CaCl2 as a separating agent, for which the Aspen Dynamics® simulator is used. The measurement and control of the ethanol composition are carried out by means of temperature, in addition, four control structures are evaluated and compared. These structures are L, D, LV, and DV, which are the most common in conventional distillation, and their performance is measured by means of deterministic indicators applying changes (disturbances) of composition and the flow rate in the main feed of the column. The most relevant resul... [more]

In this paper, a method is proposed to accurately measure the torsional vibration of a ship’s propulsion shafting, and calculate its instantaneous torque based on instantaneous speed data. The instantaneous speed signal of a ship is measured by a data acquisition instrument indirectly under the deceleration condition, and then filtered by the five-point smoothing algorithm. The filtered instantaneous speed signal is further processed to calculate the maximum amplitude of the torsional vibration, along with the maximum torque of the propulsion shafting under specific operating conditions. The proposed method can effectively improve the calculation accuracy of the torsional vibration of the shafting, and provides reliable theoretical basis for setting the safe speed of the ship’s diesel engine.

The process of microbial spray-drying has inherent defects such as short time, complexity, and non-visualization of particle trajectory. However, there has been a lack of effective methods for real-time sampling, rehydration, and non-destructive storage of dried particles, as well as mathematical modeling of the drying process of yeast particles based on sampling and measurement data. Therefore, firstly, a real-time sampling system was developed which completed real-time sampling, rehydration, and non-destructive storage of spray-dried particles, and realized the real reproduction of the changes of yeast particles in the process of spray drying. The laws that the motion trajectory of microbial particles during spray drying are divided into the first cycle region and the reflux cycle region were concluded, and the partition mechanism was explored. Then, based on the sampling data and the law of heat and mass transfer, a mathematical model of porous media was established to predict the v... [more]

In this research, the extraction with supercritical carbon dioxide (SC-CO2) and the subsequent impregnation of the extracted bioactive compounds from Zataria multiflora Boiss (Z. multiflora) into polylactic acid (PLA) films was investigated. The effects of temperature (318 and 338 K), pressure (15 and 25 MPa) and cosolvent presence (0 and 3 mol%) on the extraction yield were studied. The SC-CO2 assisted impregnation runs were carried out in a discontinuous mode at different pressure (15 and 25 MPa), temperature (318 and 328 K), and time (2 and 8 h) values, using 0.5 MPa min−1 as a constant value of depressurization rate. ANOVA results confirmed that pressure, temperature, and time influenced the extraction yield. Moreover, antioxidant activities of extracts of Z. multiflora were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. In addition, the antibacterial activities of the extracts were screened against standard strains of Staphylococcus aureus (S. aure... [more]

is a prominent emerging pathogen responsible for a variety of hospital-acquired infections. It can contaminate inanimate surfaces and survive in harsh environmental conditions for prolonged periods of time in the form of biofilm. Biofilm is difficult to remove with only one method of disinfection, so combined disinfection methods and biocidal active substances are needed for biofilm eradication. Additionally, having in mind ecological demands, legislators are more prone using fewer toxic substances for disinfection that produce less solid waste and hazardous disinfection byproducts. Gaseous ozone and citric acid are natural biocidal compounds, and the purpose of this study was to determine their combined biocidal effects on A. baumannii biofilm formed on ceramics and polystyrene. Twenty-four-hour A. baumannii biofilm formed on ceramic tiles and polystyrene was exposed to different combinations of disinfection protocols with 25 ppm of gaseous ozone for 1 h exposure time and 15% citric a... [more]

High gassy mines have low recovery rates of coal resources in the stoping of high-seam coal resources in addition to difficulties in the gas control of the working face and gas accumulation in the goaf and upper corner. Pillar-free gob-side entry-retaining technology was combined with the high-pumping roadway for gas control in the goaf based on the W1319 working face in the Gaohe Coal Mine. Theoretical analysis and numerical simulation were used to derive the width of the reserved coal pillar in the high-gas pillar-free working face and the horizon of the high-pumping roadway. The location of the high-pumping roadway was determined in combination with the on-site investigation. Numerical simulations were used to compare the plastic failures and deformations of surrounding rocks in the cases of non-roof-cutting, roof cutting, and reinforced roof cutting. It could solve large and uncontrolled surrounding rock deformations of the gob-side entry-retaining technology and return airway in t... [more]

In recent years, the permutation flowshop scheduling problem (PFSP) with sequence-dependent setup times has been widely investigated in the literature, most focusing on the single-objective optimization problem. However, in a practical production environment, schedulers usually need to handle several conflicting objectives simultaneously, which makes the multiobjective PFSP with sequence-dependent setup times (MOPFSP-SDST) more difficult and time consuming to be solved. Therefore, this paper proposes a learning and swarm based multiobjective variable neighborhood search (LS-MOVNS) for this problem to minimize makespan and total flowtime. The main characteristic of the proposed LS-MOVNS is that it can achieve the balance between exploration and exploitation by integrating swarm-based search with VNS in the multiobjective environment through machine learning technique. For example, the learning-based selection of solutions for multiobjective local search and the adaptive determination of... [more]

plants are of great interest to traditional medicine in the history of several civilizations, particularly in terms of chronic human diseases. Essential oils obtained from this genus have also been used as therapeutic agents. In this present work, two plants of the Ocimum species from Djibouti, Ocimum basilicum L. and Ocimum americanum L., were subjected to hydrodistillation to obtain their essential oils. Gas chromatography-mass spectrometry was performed to determine the chemical composition of both essential oils. Linalool (41.2%) and estragole (30.1%) are the major compounds among the 37 compounds that have been identified in the essential oil of Ocimum basilicum L. (EOOB), and carvotanacetol (38.4%) and estragole (27.5%) are the main compounds among the 42 compounds that have been identified in the essential oil of Ocimum americanum L. (EOOA). Morever, the cytotoxic activity of EOs was evaluated against 13 human cancer cell lines (K562, A549, HCT116, PC3, U87-MG, MIA-Paca2, HEK293... [more]

Vaccine supply has a bottleneck in manufacturing capacity due to operation personnel and chemicals needed. Assessment of existing mRNA (messenger ribonucleic acid) vaccine processing show needs for continuous manufacturing processes. This is enabled by strict application of the regulatory demanded quality by design process based on digital twins, process analytical technology, and control automation strategies in order to improve process transfer for manufacturing capacity, reduction out-of-specification batch failures, qualified personnel training and number, optimal utilization of buffers and chemicals as well as speed-up of product release. In this work, process control concepts, which are necessary for achieving autonomous, continuous manufacturing, for mRNA manufacturing are explained and proven to be ready for industrialization. The application of the process control strategies developed in this work enable the previously pointed out benefits. By switching from batch-wise to cont... [more]

In the last decades, the increasing complexity of industrial information technology has led to the emergence of new trends in manufacturing. Factories are using multiple Internet of Things (IoT) platforms to harvest sensor information to improve production. Such a transformation contributes to efficiency growth and reduced production costs. To deal with the heterogeneity of the services within an IoT system, Service-Oriented Architecture (SOA) is referred to in the literature as being advantageous for the design and development of software to support IoT-based production processes. The aim of SOA-based design is to provide the leverage to use and reuse loosely coupled IoT services at the middleware layer to minimise system integration problems. We propose a system architecture that follows the SOA architectural pattern and enables developers and business process designers to dynamically add, query or use instances of existing modular software in the IoT context. Furthermore, an analysi... [more]

When a liquid flows, it has an internal resistance to flow. Viscosity is the property that measures this resistance, which is a fundamental characteristic parameter of liquids. The monitoring of viscosity is essential for quality control in many industrial areas, such as the pharmaceutical, chemical, and energy-related industries. Several instruments measure the viscosity of a liquid, the most used being the capillary viscometers. These instruments are complex, associated with high cost and expensive prices. This represents a challenge in several industries, where accurate viscosity knowledge is essential in designing various industrial equipment and processes. Using image processing and machine learning algorithms is a promising alternative to the current measurement methods. This work aims to extract characteristic information from videos of droplets of different samples using image processing algorithms. An Artificial Neural Network model utilizes the extracted characteristics to cl... [more]

The production of food is highly complex due to the various chemo-physical and biological processes that must be controlled for transforming ingredients into final products. Further, production processes must be adapted to the variability of the ingredients, e.g., due to seasonal fluctuations of raw material quality. Digital twins are known from Industry 4.0 as a method to model, simulate, and optimize processes. In this vision paper, we describe the concept of a digital food twin. Due to the variability of the raw materials, such a digital twin has to take into account not only the processing steps but also the chemical, physical, or microbiological properties that change the food independently from the processing. We propose a hybrid modeling approach, which integrates the traditional approach of food process modeling and simulation of the bio-chemical and physical properties with a data-driven approach based on the application of machine learning. This work presents a conceptual fra... [more]

Cell aggregates have been applied in various fields such as regenerative medicine and drug toxicity testing. We have shown that H-(Lys-Pro)12-OH (KP24), a repeating sequence of lysine (Lys) and proline (Pro), induces uniformly sized cell aggregates simply by its presence in cell suspension. In this study, we considered that this peptide could be applied to a three-dimensional culture substrate that can induce uniform cell aggregates by immobilizing it on the substrate. Therefore, mouse fibroblasts (L929) were seeded on KP24-immobilized glass substrates and cell behavior was observed. We also seeded human-derived cells, namely, human mesenchymal stem cells (hMSC), on KP24-immobilized substrates and characterized their cell assemblies. Furthermore, KP24 was chemically immobilized on the substrate surface, which allowed us to trace the mechanism of KP24−cell interaction. As a mechanism analysis of the cell aggregation ability of KP24, we investigated whether KP24 interacts with the cell s... [more]

A coupled reservoir/wellbore simulator is essential for solving relevant flow phenomena with dynamic interactions between reservoir and wellbore in two-phase flow systems. A reservoir simulator or wellbore simulator alone fails to solve these transient flow phenomena. This paper summarises a critical review of the coupled reservoir and wellbore simulation. First, a wide application of the drift-flux (DF) models to simulate gas−liquid flow in a wellbore model coupling to a reservoir model was discussed. Then, the mechanisms of the coupled reservoir/wellbore simulator in two-phase flow systems were discussed, including the reservoir modeling, wellbore modeling, and their coupling schemes. Various examples of representative coupled simulators were presented. Finally, some case studies were reported to reveal dynamic interactions between wellbore and reservoir by using the coupled reservoir/wellbore simulators. This study gives the most up-to-date and systematic sights on the relevant rese... [more]

Surface soils form a major planting base of winter jujube in China were collected and detected for six heavy metals including Co, Ni, Cu, Zn, Cd, and Pb. The concentrations of Co, Ni, Cu, Zn, Cd, and Pb were 27.6 ± 6.0, 57.9 ± 12.8, 67.1 ± 10.3, 102.6 ± 23.4, 0.24 ± 0.07, and 25.1 ±5.9 mg/kg, respectively, showing an order of Zn > Cu > Ni > Co > Pb > Cd. The contents of the investigated metals were frequently observed higher than their related background values, suggesting that extra metal inputs occurred. Levels of all elements were below the associated risk screening values of agricultural soil in China, indicating healthy planting conditions for the winter jujube cultivation. Nemerow comprehensive pollution indexes of the metals in all the sampling stations were lower than 0.7, revealing a non-pollution status of the soils. Geo-accumulation indexes suggested that Zn and Pb caused no pollution, and Co, Ni, Cu, and Cd seemed to result in slight pollution. Co, Ni, Zn, Cd, and Pb had si... [more]