Cell-free synthetic biology is advancing with unprecedented control and design. The development of cell-free biosynthesis involves both pure enzyme and crude enzyme systems. The relatively cheap crude enzyme system is more suitable for the scientific research needs of ordinary laboratories. The key factor in giving full play to the advantages of the system is to obtain high-quality cell crude extract and its energy regeneration system, but there is no systematic report on the development history of these two aspects. Therefore, in this paper, the development history of the process of obtaining crude extract from cell-free biosynthesis was carried out based on Escherichia coli, which is widely used at present, and the energy regeneration system was briefly introduced. Finally, the challenges of current cell-free synthetic systems are discussed.

Surface modification is crucial to the fabrication of (multi)functional materials and interfaces for a range of applications, such as superhydrophobic and self-cleaning surfaces, anti-biofouling and antibacterial coatings, dropwise condensation, packaging materials, sensors, catalysis, and photonics [...]

A plant cell-based recombinant glucocerebrosidase was approved by the FDA in 2012 for the treatment of human inherited Gaucher disease, indicating that plant suspension cells have advantages in biosafety and a low production cost as a commercial pharmaceutical recombinant protein expression system. A low allergenic rice suspension cell-based recombinant protein expression system controlled by the αAmy3/RAmy3D promoter has been shown to result in relatively high protein yields in plant cell-based systems. Although several recombinant proteins have been produced in rice suspension cell-based systems, yields must be improved to compete with the current commercial protein expression systems. Different strategies were performed and showed successful improvements in recombinant protein yields in this rice system. The review updates and highlights strategies for potential improvements of the αAmy3-based rice suspension cell-based system.

The present study concerns investigating the two-dimensional Magnetohydrodynamics (MHD) boundary layer flow of Williamson nanofluid over a non-linear stretching sheet. The focus of this study is based on the global influence of the non-Newtonian Williamson fluid parameter (λ) rather than the local one that exists in the literature for linear and non-linear stretching cases. The mathematical model of the problem is based on the law of conservation of mass, momentum, and energy. The derived partial differential equations are transformed into ordinary differential equations by applying an appropriate similarity transformation. The subsequent equations are solved numerically by using the Shooting method. The physical quantities Skin friction coefficient, as well as the Sherwood and Nusselt numbers are computed locally. To validate the implemented shooting method, a comparison is made with the results obtained by Matlab function bvp4c, and good agreement is found. The Prandtl number, Pr, ha... [more]

Exogenously applied phytohormones improve the endosperm cells and establish greater kernel sink capacity and grain filling, improving grain yield. In this study, 28-Homobrassinolide (HBR) dosages (20, 25, and 30 mg a. i. ha−1) were applied separately at the silking stage under controlled conditions, and EDAH (a mixture of ethephon and diethyl aminoethyl hexanoate) dosage of 90 g a. i. ha−1 was sprayed at the jointing stage to enhance the lodging resistance. Our objective was to investigate whether the application of HBR under controlled conditions or with EDAH could enhance the grain filling rate by regulating endogenous hormones. The results showed that HBR at the silking stage significantly increased endogenous hormones (ABA, IAA, Z+ZR), hampered leaf senescence, enhanced photosynthetic, improved dry matter accumulation in grains, and increased the grain-filling period, filling rate, and thousand-grains weight. Additionally, HBR 25 and 30 mg a. i. ha−1 increased the final yield by 9.... [more]

Pharmaceutical CEC compounds are a potential threat to man, animals, and the environment. In this study, a sol−gel-derived TiO2 (SynTiO2) was produced and subsequently sonochemically doped with a 1.5 wt% Mo to obtain the final product (Mo (1.5 wt%)/SynTiO2). The as-prepared materials were characterized for phase structure, surface, and optical properties by XRD, TEM, N2 adsorption−desorption BET isotherm at 77 K, and PSD by BJH applications, FTIR, XPS, and UV-Vis measurements in DRS mode. Estimated average crystallite size, particle size, surface area, pore-volume, pore size, and energy bandgap were 16.10 nm, 24.55 nm, 43.30 m2/g, 0.07 cm3/g, 6.23 nm, and 3.05 eV, respectively, for Mo/SynTiO2. The same structural parameters were also estimated for the unmodified SynTiO2 with respective values of 14.24 nm, 16.02 nm, 133.87 m2/g, 0.08 cm3/g, 2.32 nm, and 3.3 eV. Structurally improved (Mo (1.5 wt%)/SynTiO2) achieved ≈100% carbamazepine (CBZ) degradation after 240 min UV irradiation under... [more]

Bioactive peptides with high potency against numerous human disorders have been regarded as a promising therapy in disease control. These peptides could be released from various dietary protein sources through hydrolysis processing using physical conditions, chemical agents, microbial fermentation, or enzymatic digestions. Considering the diversity of the original proteins and the complexity of the multiple structural peptides that existed in the hydrolysis mixture, the screening of bioactive peptides will be a challenge task. Well-organized and well-designed methods are necessarily required to enhance the efficiency of studying the potential peptides. This article, hence, provides an overview of bioactive peptides with an emphasis on the current strategy used for screening and characterization methods. Moreover, the understanding of the biological activities of peptides, mechanism inhibitions, and the interaction of the complex of peptide−enzyme is commonly evaluated using specific in... [more]

Grape pomace (GP) is a good source of high-value compounds as up to 60% of grape polyphenols remain in it after wine-making. To overcome traditional membrane technologies’ d rawbacks, such as fouling, a novel Zero Head Space extraction (ZHE) procedure was developed. The reaction vessel comprised a filtration device with a nitrocellulose membrane. The separation was performed at 50 lb/in2 and 23 °C, with no headspace during the process. Water and methanol (both acidifie d) were evaluated as solvents during two extraction stages for the recovery and fractionation of polyphenols. Aqueous extract (AE) was mainly constituted by monomeric polyphenols while Methanol extract (ME) presented less soluble compounds, as well as a higher concentration of total anthocyanin content than AE. Additional methanolic (CE) and acetone (CAE) extractions of residual GP showed CE presented a similar profile to ME (at a lower concentration), indicating ZHE efficiency at extracting polyphenols in GP. CAE presen... [more]

Natural gas hydrate (NGH) is a kind of unique resource among hydrocarbon fuels. It is stable within its sedimentary hosts and will only convert to its constituent gas and water phases relatively quickly if reservoir pressure-temperature conditions are artificially disturbed. The unwanted vibrations from the drill string with the complex external environment and excitation usually cause instability in the NGH. In this paper, a nonlinear mathematical model is presented taking into account the fluid−solid coupling vibration caused by the drilling fluid inside and outside the drill string, the non-smooth rub-impact between the drill string and borehole wall, and the influence of centralizer setting interval on nonlinear resonant vibration of the drill string. The relationship between the variable parameters of the drill string and the complex dynamics of the drill string is analyzed. Finally, the bifurcation diagram is given. The results show that the system response exhibits complex dynam... [more]

A mulberry-like MnO specimen was prepared using a MnCO3 sample under nitrogen (N2) protection at 700 °C (denoted as MnO-700). When the specimen was used in lithium-ion batteries (LIBs) as anode material, the reversible capacity of 702 mAh g−1 was displayed after 120 cycles at a current density 200 mA g−1, and 365 mAh g−1 of discharge capacity was obtained at 1000 mA g−1 at the 200th cycle. Meanwhile, the sample also exhibited an excellent rate capacity (224 mAh g−1 at 2000 mA g−1). The MnO-700 sample displayed a favorable electrochemical performance that may be ascribed to the unique mulberry-like structure of the MnO microparticles, which can provide enough space to satisfy the volume change of the MnO microparticles during lithium cycling, and also lead to more transfer paths for Li+ insertion/extraction during charge/discharge processes.

African agriculture is adversely impacted by arable soil compaction, the degree of which is affected by the speed at which the tractor is maneuvered on the fields, which affects the degree of soil compaction. However, there is no reliable, existing mathematical correlation between the extent of compaction on the one hand, and the tractor speed/s and soil moisture levels on the other. This paper bridges this gap in knowledge by resorting to the artificial neural networks (ANNs) method to predict the effects of tractor speed and soil moisture on the state of soil compaction. The models were ‘trained’ with penetration resistance (CPR) and bulk density test data obtained from field measurements. The resulting correlation coefficient (R = 0.9) showed good compliance of the prediction made with the ANN models with on-field data. It follows, thereby, that the model developed by the authors in this study can be effectively used for predicting the effects of speed, soil density, and moisture co... [more]

Industries in different sectors are addressing the emission reductions of their processes. Several energy efficiency proposals have been developed by researchers. However, the complexity of most industrial sectors makes it difficult for modelers, businesses, and policymakers to appreciate and realize the full potential for efficiency-driven energy savings in specific industries. Thus, this paper studies the energy efficiency potentials within a vehicle store in Quito, Ecuador. Data on power consumption, operating hours, and consumption were recorded for each inventory item. The workshop was identified as a problem area when the data were analyzed by agency action areas. It is the part of the company with the most machinery and the most operational hours. Finally, proposals for energy efficiency were developed and examined using the internal rate of return and net present value. The proposals with the best applicability were specified using these two indications, resulting in the changi... [more]

Translational medicine, the exchange between laboratory (bench) and the clinic (bedside), is decidedly taking on a vital role. Many companies are now focusing on a translational medicinal approach as a therapeutic strategy in decision making upon realizing the expenses of drug attrition in late-stage advancement. In addition, the utility of biomarkers in clinical decision and therapy guidance seeks to improve the patient outcomes and decrease wasteful and harmful treatment. Efficient biomarkers are crucial for the advancement of diagnoses, better molecular targeted therapy, along with therapeutic advantages in a broad spectrum of various diseases. Despite recent advances in the discovery of biomarkers, the advancement route to a clinically validated biomarker remains intensely challenging, and many of the candidate biomarkers do not progress to clinical applications, thereby widening the innovation gap between research and application. The present article will focus on the clinical vie... [more]

When drilling to obtain hydrocarbons (oil and natural gas), we cannot underestimate the anomalously high pressures in the deposit layers, as these pressures can cause an uncontrollable eruption. Therefore, it is important to look for signs of anomalous high contour pressures over time, which, according to a detailed analysis, could be used to predict and quantify high formation pressures. These arise under conditions of intense vertical migration of formation fluids, where the liquids in the well have to carry part of the weight of overlying rocks and are often also related to tectonic activity. The main aim of the present study was to detect the emergence of a gas kick, which, as a result of an improper technological procedure, can cause an uncontrollable eruption, which can lead to a total accident of the well. In this article, we describe the use of modern drilling technology and sophisticated software that displays the current status inside the well. These can reveal impending pres... [more]

The coffee agribusiness generates significant amounts of waste that becomes an environmental problem in producing countries. For example, synthetic coagulants have sustainability disadvantages. Immature coffee beans are collected together with mature beans, and their high polyphenol content makes them unsuitable for coffee production and commercialization. This paper aims to test the coagulant activity of polyphenols extracted from Coffea arabica residues in synthetic water samples to use them as raw material for producing a natural coagulant based on bioeconomy. It would thus allow immature coffee beans to recover, avoiding their inadequate disposition. An extract was obtained from residual green coffee beans using the ultrasound-assisted separation technique with a mixture of ethanol and water in a 1:1 ratio. The Folin−Ciocalteu method was applied for the total polyphenols quantification, resulting in a concentration of 73.54 ± 0.05 mg GAE (Gallic Acid Equivalent) per gram on a dry c... [more]

To improve the safety of electric vehicles, this paper analyzes the way of cooling lithium-ion batteries of electric vehicles and proposes an air-cooling scheme. First, a heat-generation model for the lithium-ion battery is prepared for numerical simulation and a finned air-cooling model is designed, which combines cold air with fins to lower the temperature of lithium-ion batteries. Through feasibility analysis, it is proved that the heat-generation model can reflect the actual heat generation of lithium-ion batteries. The cooling effect of the finned air-cooling model is also evaluated. The temperature can be 23.6 °C lower than the maximum compared with lithium-ion batteries without cooling. The cooling effect is desirable for diamond, triangle and rectangular lithium-ion battery packs, among which the rectangular battery pack achieves the most desirable effect. To conclude, to ensure the safe operation of lithium-ion batteries in electric vehicles, air cooling can be used to control... [more]

In precision seeding, the final displacements of the seeds are determined as a working result of the profiling mechanism, opener, seed-metering device, covering apparatus and compacting machine. For a better understanding of the disturbance of seed displacement during soil covering and compaction in the actual working process, experiments and simulations have been performed. In this paper, a type of soybean seeding monomer was taken as the research object, and a soil bin test of soil covering and compacting was executed. The experimental results showed that the traction velocity and the open angle of the covering discs had a significant influence on the changes in the horizontal and vertical displacements of seeds during the soil covering processing. With an increasing traction velocity, the vertical displacements of seeds increased after soil covering; in contrast, the horizontal displacements decreased. When the covering apparatus had a larger open angle it had a smaller disturbance... [more]

The three-phase separator is one of the most important pieces of equipment in the combined station of the oilfield. The control level of the oil−water interface directly affects the energy consumption of the subsequent production of the combined station and the effect of oil, gas and water separation. In order to avoid these situations, the Siemens PLC control system, configuration software WinCC and MATLAB were used. The OPC technology is used to connect communication between WinCC and MATLAB, and the genetic algorithm in MATLAB is used to obtain the optimal separation height of the oil−water interface under the produced liquid in different periods. Subsequently, through the Siemens PLC system and WinCC configuration software, the automatic control of the three-phase separator is achieved, and finally the water content of crude oil is significantly reduced. The system provides a visual interface function. In the future, it will also provide an effective simulation platform for the the... [more]

Mold with diameter sizes of 140 mm, 80 mm, 40 mm and 15 mm were designed to obtain the ingot of Fe-30Mn-10Al-1.1C low-density steel under different cooling rates. The influence of cooling rate on the grain morphology and elemental segregation behavior during the steel solidification process was analyzed by methods including inductively coupled plasma, scanning electron microscope and energy dispersive spectroscopy. The solidification sequence of the low-density steel was calculated by JMatPro 7.0 thermodynamic software. The results show that the microstructure of the steel is mainly austenite and contains a small amount of ferrite. The solidification order in the steel is: L → α, L → γ and α → γ, L → γ + MC. As the cooling rate increases from 1.69 °C/s to 10.28 °C/s, the ferrite phase precipitation increases by 16.7%, and the grain size decreases significantly, and in particular, the austenitic grain size decreases by 26%. With the increase in cooling rate, the microscopic segregation... [more]

The sealing system performance of a nuclear reactor pressure vessel (RPV) under different working circumstances is vital to the safe operation of the whole nuclear process; however, the mechanism, and especially the relationship among highly related variables for it, has remained unclear. Therefore, a new inconsistent knowledge fused Bayesian network and weighted loss function (iBWL) method was proposed to identify key variables and estimate the reliability of an RPV sealing system. In this method, a new inconsistent knowledge fusion method was proposed to make good use of available priceless field knowledge by considering its reliability and inconsistency seriously. The key variables identified by the Bayesian network structure were then used by a new weighted loss function to estimate the reliability of the RPV sealing system by comprehensively quantifying the deviations of them from their corresponding expected values. It is not only the quantified reliability of RPV sealing that ca... [more]

In recent years, CFD methods have become a universal engineering tool for modeling many classes of flows [...]

LC-MS/MS uses information on the mass peaks and peak areas of samples to conduct quantitative analysis. However, in the detection of clinical samples, the spectrograms of the compounds are interfered with for different reasons, which makes the identification of chromatographic peaks more difficult. Therefore, to improve the chromatographic interference problem, this paper first proposes a multi-window-based signal-to-noise ratio estimation algorithm, which contains the steps of raw data denoising, peak identification, peak area calculation and curve fitting to obtain accurate quantitative analysis results of the samples. Through the chromatographic peak identification of an extracted ion chromatogram of VD2 in an 80 ng/mL standard and the spectral peak identification of data from an open-source database, the identification results show that the algorithm has a better peak detection performance. The accuracy of the quantitative analysis was verified using the LC-HTQ-2020 triple quadrupo... [more]

The present research describes the design, analysis, and modeling of an air-granular particle fluidized-bed system with dimensions of 0.08 m × 0.4 m × 0.08 m. The hydrodynamic and thermal experiments are designed to verify the numerical model previously created for this purpose. The gas-solid two-phase flow is described using a three-dimensional, two-fluid model based on the Eulerian−Eulerian method. The experiment is conducted, and the numerical model is updated for the new geometry while maintaining the solution parameters. Silica sand and sintered bauxite particles are employed in both experimental and numerical investigations to examine the behaviors of these particles. The hydrodynamic validity of the numerical model is established by the use of experimental findings for pressure drop and bed expansion ratio. The thermal tests are conducted with 585 K hot sand, and the temperature distribution in the bed is measured using K-type thermocouples and compared with the simulation data.... [more]

The last mile constitutes the most expensive and difficult part of the supply chain. This fact results from the density of buildings and from traffic congestion. The implemented programs that aim at increasing the effectiveness of city logistics do not always bring about the expected results, the reason being the lack of consultations with stakeholders prior to their implementation. Such consultations could help to recognize the expectations, aims and fears of each of the agent/actor groups using the urban space. The purpose of this article is to recognize the attitude of one of the groups of stakeholders (recipients/businesses located in the area) in the Polish neighboring agglomerations of Gdańsk and Gdynia, towards chosen city logistic measures. The research consisted in searching for a correlation between the attitude towards a particular city logistics measure and the size of the business, as well as between the attitude towards the given city logistics measure and the difficulty... [more]

The iterative design process of mechanical products has evolved towards a more sustainable direction driven by the concept of sustainable development. Modular design emphasizes the realization of user requirements under the condition of high efficiency and low cost, which is conducive to the diversification of product design. The sustainable innovative design methods are considered in modular design processes, which can improve the design capabilities of enterprises and enhance competitiveness. However, although the existing methods used in the iterative design of a product scheme contributes to satisfy the user requirements and generate the innovative scheme of the product, the sustainable design concepts such as the impact on the utilization of resources and environment during the iteration of the conceptual scheme and sustainability evaluation of product schemes have not been given sufficient attention. To resolve this, in this work, an iterative conceptual design process is propose... [more]