SUMOylation is a reversible covalent process between a small ubiquitin-like modifier (SUMO) and its target protein and has become a crucial regulator of protein functions. Here, we report that Bombyx mori nucleopolyhedrovirus (BmNPV) may take advantage of the host SUMOylation system to enhance its own replication, similar to many other viruses. Both the knockdown of BmSUMO by RNAi and chemical blocking by ginkgolic acid both impaired BmNPV replication. Using site mutation and pull-down assays, we found that lysine K70 of the protein kinase-interacting protein (PKIP), which is conserved in all Alphabaculoviruses, was modified by SUMO. Mutation of K70 in PKIP led to its translocation from the cytoplasm to the nucleus. Knockout and rescue experiments showed that the rescue of PKIP mutant virus with wild-type PKIP restored BmNPV replication to the normal level, but this was not true for the K70R mutation. Altogether, these results show that SUMOylation of PKIP plays a key role in BmNPV rep... [more]

We consider one-step and two-step simple models of anaerobic digestion that are able to adequately capture the main dynamical behaviour of the full anaerobic digestion model ADM1. We do not consider specific growth functions. We only require them to satisfy certain qualitative assumptions. These assumptions are satisfied for concave growth functions, but they are also satisfied for a large class of growth functions found in many applications. We consider the maximisation of the biogas production with respect to the operating parameters of the model, which are the dilution rate and the substrate input concentration. We give the best operating conditions and we describe them as a subset of the set of operating parameters. Our models incorporate biomass decay terms, corresponding to maintenance. Numerical plots with specified growth functions and biological parameters illustrate the obtained results.

Wheat alpha-amylase/trypsin inhibitors remain a subject of interest considering the latest findings showing their implication in wheat-related non-celiac sensitivity (NCWS). Understanding their functions in such a disorder is still unclear and for further study, the need for pure ATI molecules is one of the limiting problems. In this work, a simplified approach based on the successive fractionation of ATI extracts by reverse phase and ion exchange chromatography was developed. ATIs were first extracted from wheat flour using a combination of Tris buffer and chloroform/methanol methods. The separation of the extracts on a C18 column generated two main fractions of interest F1 and F2. The response surface methodology with the Doehlert design allowed optimizing the operating parameters of the strong anion exchange chromatography. Finally, the seven major wheat ATIs namely P01083, P17314, P16850, P01085, P16851, P16159, and P83207 were recovered with purity levels (according to the targete... [more]

Date palm fruits (Phoenix dactylifera) contain high levels of fructose and glucose sugars. These natural sugar forms are healthy, nutritional and easily assimilate into human metabolism. The successful production of soluble date sugar powder from nutritious date fruits would result in a new food product that could replace the commercial refined sugar. In this work, a novel process technology based on the supercritical extraction of sugar components from date pulp was modeled and simulated using Aspen Plus software. The process model consisted of three main steps that were individually simulated for their optimal working conditions as follows: (a) freeze-drying of the date pulp at −42 °C and 0.0001 bar; (b) supercritical extraction of the sugar components using a 6.77 wt.% water mixed CO2 solvent system at a pressure of 308 bar, temperature of 65 °C, and CO2 flow rate of 31,000 kg/h; and (c) spray-drying of the extract using 40 wt.% Gum Arabic as the carrier agent and air as drying medi... [more]

Grouting is a reinforcing method commonly used in underground engineering. The grouting-reinforced body is in saturated groundwater, which is due to long-term seepage, resulting in reinforcing performance attenuation. This is a guarantee for the safe operation of the underground structure’s long life cycle that obtains the performance attenuation law of the grouting-reinforced body under the action of seepage. This paper uses primitive to describe the mechanical properties of grouting-reinforced body under seepage erosion, establishing the damage mechanics model of grouting-reinforced body under seepage, designing deterioration experimental of grouting-reinforced body under seepage, studying the mechanism of the permeation water pressure on the permeability of the grouting-reinforced body, and obtaining the variable of the grouting-reinforced body damage variable with the permeation water pressure. Studies have shown that seepage effects have a “sudden jump” phenomenon in the process o... [more]

In this work, a novel methodology to determine moisture transport coefficients for MMC PH101 tablets is presented. Absolute permeability, moisture diffusion, moisture transfer, and water vapor permeability coefficients were estimated on compressed powder tablets produced with different compression pressures (20 MPa to 200 MPa with an interval of 20 MPa). The ASTM D6539 standard test was used to measure the absolute permeability. The moisture transfer coefficient was determined from measured absolute permeability. The moisture diffusion coefficient was obtained with the tablet average pore radius, which was determined with the water droplet penetration method. Descriptive and phenomenological models derived from the measurements were confronted with existing and adopted models, and a good agreement was found. The obtained models are of the function of the microstructural properties of the tablet (average pore radius and average porosity). The tablet average porosity was found to be the... [more]

Surface roughness of the finished part and profile of the tool electrode are significant factors to assess the functionality of electrical discharge machining process. In this study, EDM was utilized for the machining of hardened EN31 steel. A sintered cermet tool tip with 75% copper−25% titanium carbide was fabricated and used as tool electrode. A data set of 262 such samples was developed with machining variables including discharge current (Ip), gap voltage (Vg), pulse on time (Ton), pulse off time (Toff) and flushing pressure (P). By correlating the machining variables, a machine learning-based regression model was developed for the prediction of surface roughness of the machined surface and change in out-of-roundness of tool during the EDM process. With the help of heat maps and a probability table, it was found that Ip, Ton, Toff and P had significant effect on SR, and Ip, Ton and Toff affected OOR. The machine learning-based regression equation predicted SR with average error of... [more]

More rigorous environmental rules on hazardous metals release require the implementation of diverse methods to remove them from contaminated streams. The adsorption process is the most attractive method for removal of toxic metal ions from different aqueous solutions. In the present study, batch and fixed bed column experiments were carried out to investigate the performance of permethylated β-cyclodextrin impregnated resin for the removal of As(V) ions from aqueous solutions. Sorption of As(V) ions was performed in groups under a variety of pH and metal concentrations. It was noted that removal efficiency was the highest (98%) at pH 6.0, and at an initial As(V) concentration equal to 0.1 mg/L. Sorption model use for experimental data indicates that the removal mechanism of As(V) by the permethylated β-cyclodextrin impregnated resin was the physical sorption. The adsorption capacity of the cyclodextrin ligand immobilized onto Amberlite XAD-4 resin was 19 mg/g. In addition, the impregna... [more]

Roadway excavation technology in underground coal mines has an important impact on mining efficiency and production safety. High-efficiency and rapid excavation of underground roadways in coal mines are important means to improve the production efficiency of coal mines. To tackle the problems of instability of roadway and support difficulties, the tail entry of panel 3105 in Mataihao Mine was used as the case study. The methods of underground investigation, theoretical analysis, and FLAC3D numerical simulation were used to analyze the stability of the surrounding rock under different yield pillar widths. Through the stress field, displacement field, and plastic zone of roadway surrounding rock, the stability of the rock surrounding the roadway under different yield pillar widths (4 m, 6 m, and 8 m) was analyzed. The results show that, with the increase in the yield pillar width, the plastic zone failure and displacement of the roadway surrounding rock are mainly manifested in the narro... [more]

A misusage of machine learning (ML) strategies is usually observed in the process systems engineering literature. This issue is even more evident when dynamic identification is performed. The root of this problem is the gradient explode and vanishing issue related to the recurrent neural networks training. However, after the advent of deep learning, these issues were mitigated. Furthermore, the problem of data structuration is often overlooked during the machine learning model identification in this field. In this scenario, this work proposes a guideline for identifying ML models for the different applications in process systems engineering, which are usually for simulation or prediction purposes. While using the proposed guideline, the work also identifies a virtual analyzer for a pressure swing adsorption unit. In these types of adsorption separations, it is usual that the measurement of the main properties is not done online. Therefore, the virtual analyzer is another contribution o... [more]

Banana peel, a little-used waste, contains a high amount of biologically active compounds. The aim of the study is to demonstrate in vitro, the antioxidant, cytotoxic, and antimicrobial effects of hydroalcoholic extracts from yellow (BP) and red (BPR) banana peels. The analysis of the extracts by Capillary Zone Electrophoresis (CZE) has confirmed the presence of several bioactive compounds. BPR has a higher in vitro antioxidant activity than BP, which correlates with a significant cytotoxic, antimicrobial effect, with a UVA/UVB rate of 0.9. In the case of BPR, the results confirm the presence of isoquercitrin and kaempferol in a 1:3 ratio. The bioactive compounds from the extracts have shown a different interaction with HCT-8 cell lines and with tested bacterial strains with pathogenic properties. The HCA analysis proved the biological value of BPR to reduce oxidative stress and its potential use in natural products.

Composite sorbent materials (IL@MOF) with a metal-organic framework (MOF) ZIF-8 and [B(CN)4]−-based ionic liquids (ILs) were produced for the first time. Characterization results indicate the successful IL impregnation and conservation of the ZIF-8 crystalline structure and morphology. The data collected from the nitrogen (N2) physisorption at 77 K suggest that these IL@ZIF-8 materials are nonporous as their textural properties, such as BET specific surface area and total pore volume, are negligible. However, CO2, CH4, and N2 adsorption/desorption measurements in the IL@ZIF-8 composites at 303 and 273 K contradict the N2 data at 77 K, given that the obtained isotherms are Type I, typical of (micro)porous materials. Their gas adsorption capacity and ultramicroporous volume are in the same order of magnitude as the pristine microporous ZIF-8. The case study [C6MIM][B(CN)4] IL revealed a high affinity to both CO2 and CH4. This compromised the selectivity performance of its respective comp... [more]

The term “marginal oil resource” refers to an oil reservoir that has hydrocarbon resource preservation but cannot meet the criteria of resources under the U.S Securities and Exchange Commission (SEC) standards. When oilfields step into their late life, most of their economic petroleum reserves have been well developed, and their focuses need to be switched to their intact marginal resources. In this paper, reservoir characteristics and key petrophysical properties of marginal oil resources are introduced to classify marginal oil resources into four types for identifying potential development opportunities. Primary recovery and its following development strategy are applied to fully utilizing their economic returns. Waterflooding, low salinity waterflooding (LSW) and enhanced oil recovery processes are reviewed to illustrate its potential uplift on oil production and application challenges such as higher clay content in marginal resources than in commercial reservoirs. An oilfield is pr... [more]

For the purpose of improving uniformity and efficiency of microwave heating, moving components are widely used. In this paper, a kind of stretching microwave oven with a conveyor belt is designed. The conveyor belt and the stretching motion of the upper surface of the microwave oven make the electric field in the microwave cavity continuously change during heating, so that the absorption pattern of materials does not remain constant. The transformation optics method is used to simulate the stretching motion of the upper wall of the microwave oven, and the implicit function method is used to simulate the translational motion of the sample on the conveyor belt. The correctness of the simulation model is validated by experiments. The heating effects for the cases of fixed heating, stretching and translational motion are compared. Finally, the heating effects for the proposed model with different heated materials are also discussed.

A general correlation for predicting the two-phase heat transfer coefficient (HTC) during condensation inside multi-port mini/micro-channels was presented. The model was obtained by correlating the two-phase multiplier, φtp with affecting parameters using the genetic programming (GP) method. An extensive database containing 3503 experimental data samples was gathered from 21 different sources, including a broad range of operating parameters. The newly obtained correlation fits the broad range of measured data analyzed with an average absolute relative deviation (AARD) of 16.87% and estimates 84.73% of analyzed data points with a relative error of less than 30%. Evaluation of previous correlations was also conducted using the same database. They showed the AARD values ranging from 36.94% to 191.19%. However, the GP model provides more accurate results, AARD lower than 17%, by considering the surface tension effects. Finally, the effect of various operating parameters on the HTC was stud... [more]

The current study aimed to develop carbomer based hydrogel dressings, incorporating ethanolic extracts of Rosmarinus officinalis aerial parts, Achillea millefolium and Calendula officinalis flowers. The pharmaceutical properties of the obtained hydrogels, as well as their texture and antimicrobial activity, were further evaluated. Five wound dressing formulations based on carbopol were prepared. The addition of the ethanolic extracts to the formulation slightly lowered the pH of the hydrogels, as expected. The Rosmarinus officinalis aerial parts extract loaded hydrogel proved to be the firmest one. In terms of consistency and viscosity, the behavior of the five hydrogels was relatively similar. Based on the texture analysis, the texture of the hydrogels has been affected to some extent by the addition of the ethanolic extracts, decreasing their consistency, firmness, and adhesiveness. The hydrogel loaded with Rosmarinus officinalis aerial parts extract and the one incorporating the ble... [more]

The rapid advancement of genetically modified (GM) technology over the years has raised concerns about the safety of GM crops and foods for human health and the environment. Gene flow from GM crops may be a threat to the environment. Therefore, it is critical to develop reliable, rapid, and low-cost technologies for detecting and monitoring the presence of GM crops and crop products. Here, we used visible near-infrared (Vis-NIR) spectroscopy to distinguish between GM and non-GM Brassica napus, B. juncea, and F1 hybrids (B. juncea X GM B. napus). The Vis-NIR spectra were preprocessed with different preprocessing methods, namely normalization, standard normal variate, and Savitzky−Golay. Both raw and preprocessed spectra were used in combination with eight different chemometric methods for the effective discrimination of GM and non-GM plants. The standard normal variate and support vector machine combination was determined to be the most accurate model in the discrimination of GM, non-GM... [more]

Improving the efficiency of coal-fired boiler is beneficial for greenhouse gas control, mainly for carbon dioxide (CO2). The low-temperature corrosion covering heating surfaces is a frequent threat for coal-fired thermal equipment. The corrosion is induced by a fouling layer, where the ash deposition and condensed acid in coal-fired flue gas react mutually. The corrosion experiments were designed to investigate the reactions of representative acid solution between basic oxides, non-basic oxides, and fly ash particles covering metal surfaces. Scanning electron microscope (SEM) equipped with energy-dispersive X-ray spectroscopy (EDS), X-ray fluorescence (XRF) and X-ray diffraction (XRD) were used to analyze the reaction particles and metal samples collected from experiments. The corrosion rates of 316L steel, 20# steel, Corten steel and ND (09CrCuSb) steel by the sulfuric solution of different concentrations with and without particles were obtained. The results showed that corrosion rate... [more]

Thermal ablation of tumors is a minimally invasive technique more and more employed in cancer treatments. The main shortcomings of this technique are, on the one hand, the risk of an incomplete ablation, and on the other hand, the destruction of the surrounding healthy tissue. In this work, thermal ablation of a spherical hepatocellular carcinoma tumor (HCC) surrounded by healthy tissue is modeled. A modified porous media-based bioheat model is employed, including porosity variability from tumor core to healthy tissue, following experimental in vivo measures. Moreover, three different protocols are investigated: a constant heating protocol, a pulsating protocol, and a new developed damage-controlled protocol. The proposed damage-controlled protocol changes the heating source from constant to pulsating according to the thermal damage probability on the tumor rim. The equations are numerically solved by means of the commercial software COMSOL Multiphysics, and the outcomes show that the... [more]

Reaction thermal runaway, caused by excessive temperatures of the reaction system, threatens the safety of operators. Latent heat storage by phase change materials (PCMs) has the advantages of high energy storage density and stable temperature during the energy storage process, which was widely applied in many fields and provides a new idea for the temperature control of thermal runaway reactions. In this study, microencapsulated phase change materials (microPCMs) with a melamine-formaldehybe (MF) resin shell was fabricated by in situ polymerization. The characterization of the micro morphology, chemical bonds, crystal structure, thermal properties, and thermal stability of microPCMs showed that the prepared microPCMs had integrated spherical morphologies and smooth surfaces, with an encapsulation ratio of approximately 70% and good thermal stability. Furthermore, taking the esterification of propionic anhydride (PA) and 2-butanol (2B) as examples, n-octadecane@MF resin microPCMs was u... [more]

Heat transfer is a crucial aspect of thermochemical conversion of pulverized fuels. Over-predicting the heat transfer during heat-up leads to under-estimation of the ignition time, while under-predicting the heat loss during the char conversion leads to an over-estimation of the burnout rates. This effect is relevant for dense particle jets injected from dense-phase pneumatic conveying. Heat fluxes characteristic of such dense jets can significantly differ from single particles, although a single, representative particle commonly models them in Euler−Lagrange models. Particle-resolved direct numerical simulations revealed that common representative particles approaches fail to reproduce the dense-jet characteristics. They also confirm that dense clusters behave similar to larger, porous particles, while the single particle characteristic prevails for sparse clusters. Hydrodynamics causes this effect for convective heat transfer since dense clusters deflect the inflowing fluid and shiel... [more]

Biological products are increasingly important, and therefore the industry has begun to adopt quality by design, as recommended by the ICH and the U.S. FDA. Smaller companies, however, have faced difficulties in employing full-scale experiments or the quality by design strategy. Thus, this study provides an alternative way to build a model from existing data with experimental software that does not require full-scale experiments. This empirical study hopes to provide a practical way to improve the efficiency of smaller biopharmaceutical companies and researchers. Moreover, the models provided here can be applied to process characterization in recombinant protein production.

Growth in the human population and intensive, large-scale farming results in a lowering in the quality of nutrition. An oversupply of food energy is often accompanied with a deficiency in micronutrients. To address this problem, the food industry provides products enriched with bioactive substances. The main challenge of this technology is the even distribution of micronutrients in the matrix of the fortified food. A possible solution to this challenge is to use stable and effective carriers. The aim of this work was to verify the applicability of native potato starch and modified starches (commonly used in the food industry) as carriers for microelements. Adsorptions were carried out in starch suspensions at a temperature below gelatinisation. The native potato starch and the modified starches (E 1404, E 1412, E 1420, and E 1422) were assessed for their effectiveness in adsorbing copper, iron, and zinc sulphates or gluconates. Pasting characteristics were analysed using a Brabender vi... [more]

The two-fluid model (TFM) coupled with the kinetic theory of granular flow (KTGF) has gradually been used for modeling dense granular flows and mixing in rotating drums in recent years. In the present paper, a review is made from the perspective of model development and model application. It is found that several frictional viscosity models were proposed to consider the enduring contact of dense particles for the specific rotating studied, but there is still a lack of a universal model. The model is validated by various experiment results and the applicability is indicated. The model is used for investigating dynamic particle flow, and the effects of the parameters on granular flow behavior and flight design. Although the model theoretically has the advantage of saving computing resources, and is suitable for industrial-scale modeling, it is found that the model is used for the research of laboratory-scale rotating drums (diameter less than 0.5 m) and has not been used for industrial r... [more]

The agricultural sector and its related production chains are good sources of residual biomass. Olive and vineyard pruning residues are present in high quantities in Italy. The limited bulk and energy densities of these biomass materials affect the harvesting and logistic costs, limiting energy and environmental sustainability. Pelletisation is the most efficient process for increasing bulk and energy densities. This study evaluates the pelletisation process of olive and vineyard prunings, pure, or blended with variable quantities of spruce sawdust. A 15 kW pelletisation system was chosen, in line with production at the farm level. The most important quality parameters of the produced agripellets were analyzed. The results of this investigation suggest that blending could valorize other biomass materials less suitable for pelletisation and reach the pellet quality required by Italian technical standards. The addition of pruning residues to spruce sawdust leads to an improvement in dura... [more]