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Showing records 32720 to 32744 of 43611. [First] Page: 1 1306 1307 1308 1309 1310 1311 1312 1313 1314 Last
32720. LAPSE:2023.6732
Nurturing Green Consumer Values and Renewable Energy Reliance through Societal Education in Uttar Pradesh for Inclusive Capacity Building
February 24, 2023 (v1)
Subject: Energy Systems
Keywords: education, green consumer values, hydro, Renewable and Sustainable Energy, societal, solar, Wind
The main focus of this research has been on examining the renewable energy sources (RESs) consumption with respect to green consumer values (GCVs). These GCVs have primarily been examined with regard to different products and services. The most significant strategy for strengthening everyone is social education. While GCVs are assumed to be nurtured through education, specific understanding about the long-term benefits of renewable energy for a sustainable life is measured with the belief that the greater the training and development the better shaped the society can be, which boosts morale and increases their energy reliance with RES, assessed by the respondents of Uttar Pradesh (UP), as the outcome of the research. The implications for the stakeholders are the long-term advantages of renewable energy for a sustainable existence, since a society may be better formed and its reliance on RESs for energy rises the more training and development it receives. The advantages are visible, tho... [more]
32721. LAPSE:2023.6731
Simulations to Eliminate Backflow Power in an Isolated Three-Port Bidirectional DC−DC Converter
February 24, 2023 (v1)
Subject: Modelling and Simulations
Keywords: backflow power, dual phase shift, isolated DC–DC converter, multi-winding DC–DC converter, reactive power, three-port converter
The aim of this work was to eliminate the backflow power present in an isolated three-port DC−DC converter. Backflow power (which is an inherent property of phase-shifted DC−DC converters) is the major contributor of circulating current the converter, which in turn is known to be the leading cause of system loss. The dual phase shift (DPS) control scheme (which alters the transformer voltage waveform at the different winding terminals) was used to reduce the backflow power. Mathematical relations for the backflow power present in the three-port converter were derived. And from this equation, an operating point in which the backflow power is zero was also obtained. This condition for zero backflow power was confirmed by simulations on PSIM. Added to this were simulation results that show other operating conditions in which zero backflow power could be obtained in the converter. In addition, equations for the power processed at the different ports of the converter were also presented, an... [more]
32722. LAPSE:2023.6730
Comparison of Corrected and Uncorrected Enthalpy Methods for Solving Conduction-Driven Solid/Liquid Phase Change Problems
February 24, 2023 (v1)
Subject: Materials
Keywords: enthalpy method, melting, Numerical Methods, phase change material, solid/liquid phase change, solidification
The numerical study of solid/liquid phase change problems represents a large and ongoing field of research with many applications. These simulations should run as fast and accurately as possible. Therefore, proceeding from previous work and findings from the literature, this study investigates enthalpy methods for solving solid/liquid phase change problems. The relationship between temperature and enthalpy is strongly non-linear and requires special treatment; iteratively corrected methods, as well as approaches that do not correct the temperature/enthalpy relationship at all or only once per time step, were considered for a one-dimensional test problem. Based on the results of this study, two solvers can be recommended, the so-called optimum approach and a simple explicit method; both provide accurate results. The explicit method is easy to program, but the optimum approach allows larger time steps and is, therefore, faster. The influence of several parameters was investigated. The me... [more]
32723. LAPSE:2023.6729
Yield and Bioenergy Quality of Maralfalfa Biomass Obtained at Different Plant Strata and Cutting Dates
February 24, 2023 (v1)
Subject: Energy Systems
Keywords: bioenergy, fixed carbon, higher heating value, plant height, proximate analysis
One viable option for meeting global energy demand is the creation of biofuels from plant species that demonstrate high biomass productivity and good energy characteristics. In this study, growth was evaluated using plant height (PH), the production of green (GB) and dry biomass (DB), and the energy quality of leaves, pods, and stems, considering apical and basal sections of maralfalfa plants at 28, 60, 90, and 140 days after applying a uniformity cut (AUC). The variables were analyzed with correlation tests and variance analyses (ANOVA) using a factorial array design; in addition, Tukey tests were performed. A steady increase in PH (72 to 239 cm) was found. The highest yield of stems was at 90 AUC (41,362 kg/ha) for GB and 140 days AUC (6331 kg/ha) for DB, and a high correlation was observed between PH and stem biomass production for both the GB (r = 0.91) and DB (r = 0.93). There was a strong correlation between higher heating value and DB from the apical stratum (r = 0.99) and the b... [more]
32724. LAPSE:2023.6728
A Novel Distributed Consensus-Based Approach to Solve the Economic Dispatch Problem Incorporating the Valve-Point Effect and Solar Energy Sources
February 24, 2023 (v1)
Subject: Energy Systems
Keywords: consensus, distributed algorithm, economic dispatch problem, incremental cost, non-smooth cost function, Optimization, renewable energy sources, valve-point loading effect
This research focused on the design of a distributed approach using consensus theory to find an optimal solution of the economic dispatch problem (EDP) by considering the quadratic cost function along with the valve-point effect of generators and renewable energy systems (RESs). A distributed consensus approach is presented for the optimal economic dispatch under a complex valve-point effect by accounting for solar energy in addition to conventional power plants. By employing the beta distribution function and communication topology between generators, a new optimality condition for the dispatch problem was formulated. A novel distributed updation law for generation by considering the communication between generators was provided to deal with the valve-point effect. The convergence of the proposed updation law was proved analytically using Lyapunov stability and graph theory. An algorithm for ensuring a distributed economic dispatch via conventional power plants, integrated with solar... [more]
32725. LAPSE:2023.6727
Experimental Study on Utilizing Silica Gel with Ethanol and Water for Adsorption Heat Storage
February 24, 2023 (v1)
Subject: Energy Systems
Adsorption heat storage is the most feasible technology for heating decarbonization, which can store large quantities of waste and renewable heat for an exceptionally long time. However, utilizing adsorption heat storage in geographical locations with sub-zero ambient conditions is challenging. Therefore, this paper experimentally investigates the use of ethanol as a working fluid paired with silica gel for adsorption heat storage and utilizes sub-zero ambient as the heat source. The heat storage characteristics, heat charging/discharging cyclic performance, and energy conversion performance via exergy analysis were determined under realistic operating conditions and benchmarked against the widely investigated silica gel/water. Ethanol adsorbate was successfully utilized as a working fluid to employ the evaporators operating under sub-zero ambient conditions. Silica gel/ethanol showed the most significant net cyclic uptake, twice that of silica gel/water. However, the physical characte... [more]
32726. LAPSE:2023.6726
Analyses of Interpolant Ion Effects on Smart Water Core Flooding in Carbonate
February 24, 2023 (v1)
Subject: Modelling and Simulations
Keywords: carbonate, interpolants, Simulation, smart water flooding
Smart water flooding (SWF) is a promising enhanced oil recovery (EOR) technique due to its economic advantages. For this process, wettability alteration is the most accepted controlling effect that leads to increased recovery factors (RFs). The main objective of this work is to investigate how the relative permeability curves’ interpolant affects the SWF mechanisms’ assessment. Wettability alteration is described by shifting these curves in simulations. Numerical simulations of core flooding tests are applied to carbonate at 114.4 °C. A comparison of oil recovery factor (RF), pH and effluent composition is performed for different injection approaches. Mg2+ and SO42− are the interpolant ions and the salinity levels range from 30 to 1 kppm. A simulation of 24 scenarios, 12 for each type of interpolant, is presented. Results show that RF changes significantly, due to salinity and composition, for each interpolant. This has a relevant influence on the interpolant. The greater the dilution,... [more]
32727. LAPSE:2023.6725
Methods and Test Benches for Cutting Tools Testing—A Review
February 24, 2023 (v1)
Subject: Materials
Keywords: abrasive rocks, conical picks, cutting resistance tests, durability tests, hard rocks, mining machines, test benches, wear rate tests
Mechanical mining is a widely used method of separating materials from the face to obtain a useful mineral (e.g., coal, metal ores, salts, and diamonds), to make underground workings (e.g., mine galleries, tunnels, and underground garages), level roads, shape slopes, or to dig ditches. Mechanical mining is applied in the mining branch, tunnelling, road, and construction industries. Depending on the mechanical properties of the rocks, most frequently described by uniaxial compression strength, various machines and tools are used. The methods of mining high-strength abrasive rocks that have been used and developed in recent years are particularly applicable to the mining of copper, gold, tungsten, platinum ores, diamond deposits, and tunnelling. In addition to rock strength, the effectiveness of the mining process is affected by abrasiveness, which influences the rate of abrasive tool wear. Therefore, in various research and development centres, but also in production companies, tools ar... [more]
32728. LAPSE:2023.6724
Measurement and Driving Factors of Carbon Emissions from Coal Consumption in China Based on the Kaya-LMDI Model
February 24, 2023 (v1)
Subject: Energy Systems
Keywords: carbon emissions, coal consumption, driving factors, Kaya-LMDI model
As the top emitter of carbon dioxide worldwide, China faces a considerable challenge in reducing carbon emissions to combat global warming. Carbon emissions from coal consumption is the primary source of carbon dioxide emissions in China. The decomposition of the driving factors and the quantification of regions and industries needs further research. Thus, this paper decomposed five driving factors affecting carbon emissions from coal consumption in China, namely, carbon emission intensity, energy structure, energy intensity, economic output, and population scale, by constructing a Kaya-Logarithmic Mean Divisia Index (Kaya-LMDI) decomposition model with data on coal consumption in China from 1997 to 2019. It was revealed that the economic output and energy intensity effects are major drivers and inhibitors of carbon emissions from coal consumption in China, respectively. The contribution and impact of these driving factors on carbon emissions from coal consumption were analyzed for dif... [more]
32729. LAPSE:2023.6723
A Quantum Approach to the Problem of Charging Electric Cars on a Motorway
February 24, 2023 (v1)
Subject: Planning & Scheduling
Keywords: battery charging, electric motor vehicle, Energy, parallel unrelated machines, power, quantum annealing, quantum approximate optimization algorithm, quantum computing, Scheduling
In this paper, the problem of charging electric motor vehicles on a motorway is considered. Charging points are located alongside the motorway. It is assumed that there are a number of vehicles on a given section of a motorway. In the motorway, there are several nodes, and for each vehicle, the entering and the leaving nodes are known, as well as the time of entrance. For each vehicle, we know the total capacity of its battery, and the current amount of energy in the battery when entering the motorway. It is also assumed that for each vehicle, there is a finite set of speeds it can use when traveling the motorway. The speed is chosen when entering the motorway, and cannot be changed before reaching the charging station. For each speed, there is given a corresponding power usage; the higher the speed, the larger the power usage. Each vehicle can only use one charger, and when its battery is full, the amount of energy is sufficient for reaching the outgoing node. We look for a feasible s... [more]
32730. LAPSE:2023.6722
A Comprehensive Simulation Study of Physicochemical and Geochemical Interactions on Immiscible CO2-LSWAG Injection in Carbonates
February 24, 2023 (v1)
Subject: Modelling and Simulations
Keywords: carbonate, CO2-LSWAG, geochemical simulation, immiscible CO2, interpolants, physicochemical simulation
Low-salinity water-alternating-CO2 (CO2-LSWAG) injection has been widely studied and employed due to its capability to promote enhanced oil recovery (EOR). However, there is no consensus on the dominant mechanisms for oil recovery in carbonates due to the extreme complexity of the oil−brine−rock interactions. This work proposes a comparative investigation of the physicochemical and geochemical effects of continuous CO2 and CO2-LSWAG immiscible injections on oil recovery in a carbonate core. Simulations were carried out using oil PVT properties and relative permeability experimental data from the literature. A comparison of SO42− and Mg2+ as interpolant ions, oil, water and gas production, pressure, and rock and fluid properties along the core and in the effluent was made. The results show a high recovery factor for CO2 (62%) and CO2-LSWAG (85%), even in immiscible conditions. The mineral dissolution and porosity variations were more pronounced for CO2-LSWAG than CO2. The simulation res... [more]
32731. LAPSE:2023.6721
Study on Intermittent Microwave Convective Drying Characteristics and Flow Field of Porous Media Food
February 24, 2023 (v1)
Subject: Food & Agricultural Processes
Keywords: evaporation interface, flow-field distribution, intermittent microwave convective drying, multiphase flow, porous media
Numerical simulations were carried out for moist, porous media, intermittent microwave convective drying (IMCD) using a multiphase flow model in porous media subdomains coupled with a forced-convection heat-transfer model in an external hot air subdomain. The models were solved by using COMSOL Multiphysics was applied at the pulse ratio (PR) of 3. Based on drying characteristics of porous media and the distribution of the evaporation interface, IMCD was compared with convection drying (CD). Drying uniformity K, velocity difference, temperature difference, and humidity difference were introduced to evaluate the performance of three models with different inlets and outlet wall curvature. The numerical results show that as the moisture content of slices was reduced to 3 kg/kg, the drying rate in IMCD was 0.0166−0.02 m/s higher than that in CD, and the total drying time of the former was 81.35% shorter than that of the latter. In the late drying stage of IMCD, the core of the sample still... [more]
32732. LAPSE:2023.6720
Analysis and Modeling of Value Creation Opportunities and Governing Factors for Electric Vehicle Proliferation
February 24, 2023 (v1)
Subject: Modelling and Simulations
Keywords: distributed energy resource, electric vehicle, EV fleet, Modelling, value streams
This research presents a comprehensive analysis of electric vehicle (EV) proliferation factors and various monetary and non-monetary value streams emerging in the EV domain. A comprehensive mathematical model is implemented to study EV proliferation and the resulting market share applicable to any geography and jurisdictional regime. Further, a novel framework is presented to analyze the interdependency between EV proliferation factors and value streams. The proposed model and framework can be leveraged to quantifiably evaluate the timeline available for grid operators to accommodate EV growth while utilizing those as Distributed Energy Resources (DERs) to improve grid reliability, commercial value, and environmental benefits. Compared to the previous studies, the analysis indicated that if all the factors which impact EV proliferation are addressed simultaneously, EV market share can surpass the internal combustion engine vehicle (ICV) in as quickly as 15−20 years. The study also high... [more]
32733. LAPSE:2023.6719
An Agent-Based Bidding Simulation Framework to Recognize Monopoly Behavior in Power Markets
February 24, 2023 (v1)
Subject: Modelling and Simulations
Keywords: locational marginal price, monopoly, power market, Q-learning, security-constrained unit commitment
Although many countries prefer deregulated power markets as a means of containing power costs, a monopoly may still exist. In this study, an agent-based bidding simulation framework is proposed to detect whether there will be a monopoly in the power market. A security-constrained unit commitment (SCUC) is conducted to clear the power market. Using the characteristics that the agent can fully explore in a certain environment and the Q-learning algorithm, each power producer in the power market is modeled as an agent, and the agent selects a quotation strategy that can improve profits based on historical bidding information. The numerical results show that in a power market with monopoly potential among the power producers, the profits of the power producers will not converge, and the locational marginal price will eventually become unacceptable. Whereas, in a power market without monopoly potential, power producers will maintain competition and the market remains active and healthy.
32734. LAPSE:2023.6718
Aluminum-Based Fuels as Energy Carriers for Controllable Power and Hydrogen Generation—A Review
February 24, 2023 (v1)
Subject: Process Control
Keywords: aluminum combustion, aluminum fuel, aluminum-water reactions, energy conversion system, Energy Storage, recyclable energy carrier
Metallic aluminum is widely used in propellants, energy-containing materials, and batteries due to its high energy density. In addition to burning in the air, aluminum can react with water to generate hydrogen. Aluminum is carbon-free and the solid-phase products can be recycled easily after the reaction. Micron aluminum powder is stable in the air and enables global trade. Aluminum metal is considered to be a viable recyclable carrier for clean energy. Based on the reaction characteristics of aluminum fuel in air and water, this work summarizes the energy conversion system of aluminum fuel, the combustion characteristics of aluminum, and the recycling of aluminum. The conversion path and application direction of electric energy and chemistry in the aluminum energy conversion system are described. The reaction properties of aluminum in the air are described, as well as the mode of activation and the effects of the aluminum-water reaction. In situ hydrogen production is achievable throu... [more]
32735. LAPSE:2023.6717
Design and Evaluation of the Elastic and Anti-Corrosion Cement Slurry for Carbon Dioxide Storage
February 24, 2023 (v1)
Subject: Process Design
Keywords: anti-corrosion, carbon dioxide storage, cement slurry, corrosion depth, elastic modulus
Carbon dioxide capture and storage is the primary way to reduce greenhouse gas emissions on a large scale. Carbon dioxide storage is the critical link of this technology, and the way in which to achieve long-term storage is a problem to be considered. The elastic and anti-corrosion cement slurry is the key for the successful storage of carbon dioxide. In order to develop the cement slurry for carbon dioxide storage, the influence of resin with both elastic and anti-corrosion properties on the performance of a cement slurry was investigated. The dispersant, retarder, and filtrate reducer suitable for the cement slurry were studied, and the performance of the designed cement slurry for carbon dioxide storage was evaluated. The experimental results show that the resin can reduce water loss and improve the elasticity and corrosion resistance of cement paste. The elastic modulus and corrosion depth of the resin cement slurry were significantly lower than those of the non-resin cement slurry... [more]
32736. LAPSE:2023.6716
SR-GNN Based Fault Classification and Location in Power Distribution Network
February 24, 2023 (v1)
Subject: Process Monitoring
Keywords: distribution systems, fault classification, fault location, graph neural network, super-resolution
Accurately evaluating the fault type and location is important for ensuring the reliability of the power distribution network. A mushrooming number of distributed generations (DGs) connected to the distribution system brings challenges to traditional fault classification and location methods. Novel AI-based methods are mostly based on wide area measurement with the assistance of intelligent devices, whose economic cost is somewhat high. This paper develops a super-resolution (SR) and graph neural network (GNN) based method for fault classification and location in the power distribution network. It can accurately evaluate the fault type and location only by obtaining the measurements of some key buses in the distribution network, which reduces the construction cost of the distribution system. The IEEE 37 Bus system is used for testing the proposed method and verifying its effectiveness. In addition, further experiments show that the proposed method has a certain anti-noise capability an... [more]
32737. LAPSE:2023.6715
The Effect of Pore Structure on the Distribution of Wet Gases in Coal Seams of Enhong Syncline, SW China
February 24, 2023 (v1)
Subject: Energy Systems
Keywords: mercury intrusion porosimetry, pore structure, preservation, wet gases
The origin of the high content of wet gases in coalbed seams is very important geologically, especially in the Enhong syncline in China. The present study focuses on the role of the material that generates the hydrocarbons. The effect of the pore structure on the generation of wet gases has not been thoroughly examined. The present paper characterizes the coal pore structure in the “wet gas area” and “dry gas area”. The pore structures in the two areas are shown to have different features, which affect the distribution of the wet gases. With respect to the pore structure parameters, coals in the wet gas area have a greater total specific surface area and pore volume in micropores. The pore structure types also differ between the two areas: the pore structures in the dry gas areas are mainly of the parallel type and reverse S type, which is favorable for the migration and dissipation of coalbed gases. The pore structure in the wet gas area is relatively closed, with poor connectivity an... [more]
32738. LAPSE:2023.6714
Analysis of Low-Density Heat Flux Data by the Wavelet Method
February 24, 2023 (v1)
Subject: Energy Systems
Keywords: data processing, heat flux sensors, HFM method, low-density heat flux, thermal resistance measurement, wavelet analysis
When evaluating the energy efficiency of buildings and implementing the necessary measures to increase energy efficiency levels, thermal technical characteristics are determined. For this purpose, in situ measurements of the thermal resistance of external enclosing structures were carried out. One of the methods most often used by researchers is the non-destructive method—the heat flow meter (HFM) method regulated by ISO 9869. In the case of surveying a building with a high level of thermal resistance, researchers are faced with low-density heat flux measurements, which is always a difficult task due to significant fluctuations and the influence of external factors on the measurement results. This is due to the fact that it is difficult to determine what is a useful signal and what is a consequence of the effects of non-stationarity and heat transfer conditions. The article provides an example of low-density heat flux measurements when determining the thermal resistance of a building a... [more]
32739. LAPSE:2023.6713
First Law Comparison of a Forced-Circulation Solar Water Heating System with an Identical Thermosyphon
February 24, 2023 (v1)
Subject: Process Control
Keywords: first law analysis, force circulated, solar fraction, solar water heating systems, thermal efficiency, thermosyphon
The main categories of solar water heating systems (SWHSs) are the thermosyphon and the forced circulation (FC). This paper presents an experiment carried out with the aim to compare the energy performance of the FC with a thermosyphon SHWS. Identical SWHSs were installed side by side at the University of West Attica in Athens, Greece. Domestic hot water load was applied to both systems via a microcontroller-based dispensing unit which mimics the demand profile. The trial period comprised the last two months of spring (April and May). For the first law assessment, two energy indicators were utilized: the solar fraction (SF) and the thermal efficiency of the system (ηth). On days with distinctive weather conditions, both systems obtained approximately equal SF and ηth values, without a specific preference between the ambient conditions and the type of SWHS. Regarding a four-day nonstop operation, the FC overperformed the thermosyphon system at both energy indicators. Namely, for the FC... [more]
32740. LAPSE:2023.6712
Review of the Impact of Biofuels on U.S. Retail Gasoline Prices
February 24, 2023 (v1)
Subject: Energy Systems
This study aims to provide a review of the state-of-the-art literature regarding the impacts and contributions of corn ethanol on retail gasoline prices in the US. For this, a systematic literature review following PRISMA statement was carried out, seeking to answer four research questions: (1) What are the main characteristics of the literature regarding the impact and contributions of ethanol on US retail gasoline prices? (2) What are the main article clusters identified in the evaluated literature? (3) What was the numerical impact of the Volumetric Ethanol Excise Tax Credit/Renewable Fuel Standard (VEETC/RFS) mandate on the price of gasoline and what are the main methods used for calculation in the literature? (4) What are the main trends and possibly new research directions for this literature? As a result of the characterization of the sample, driving themes, such as energy policy, costs, price dynamics, trade and energy market, were identified. Furthermore, three main clusters w... [more]
32741. LAPSE:2023.6711
Constructal Evaluation of Polynomial Meta-Models for Dynamic Thermal Absorptivity Forecasting for Mixed-Mode nZEB Heritage Building Applications
February 24, 2023 (v1)
Subject: Process Design
Keywords: constructal law, Hoke D6 design, mixed-mode building, nZEB, thermal comfort
The intelligent and appropriate regulation of indoor temperatures within heritage buildings is crucial for achieving nearly Zero-Energy Building (nZEB) standards, since the technical improvement of the envelope and the overall shape of heritage buildings should be very limited in order to preserve the buildings’ authenticity. Thermal comfort is a very important factor that influences the energy performance of a building and the wellbeing of its end users. The present paper focuses on the development of a dynamic thermal human stress model that aimed to accurately predict the necessary garment insulation within a typical high-inertia heritage building. Two different statistical approaches (a Hoke D6 design and a composite factorial design) were employed for the development of this meta-model adapted to a typical mixed-mode heritage building seeking to obtain nZEB classification. Thermal human stress was modeled through the prediction of the thermal absorptivity (b) in accordance with th... [more]
32742. LAPSE:2023.6710
Sol-Gel Derived Di-Ureasil Based Ormolytes for Electrochromic Devices
February 24, 2023 (v1)
Subject: Energy Systems
Keywords: 1-butyl-3-methylimidazolium chloride, di-ureasil electrolytes, electrochromic devices, lithium tetrafluoroborate, sol-gel
Two di-ureasils incorporating oxyethylene segments with average molecular weights Y = 600 and 900 g mol−1, prepared by the sol-gel method, and doped with the ionic liquid 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and lithium tetrafluoroborate (LiBF4) salt were prepared. The as-obtained films are translucent, flexible, and hydrophobic, and have a low level of nanoscale surface roughness. The ionic conductivity values exhibited by an optimized sample are 8.10 × 10−5 and 2.8 × 10−4 S cm−1 at room temperature and 55 °C, respectively. The main goal of the work was to employ the electrolytes in prototype electrochromic devices (ECDs) with the [glass/a-IZO/a-WO3/d-U(Y)LiBF4-[Bmim]Cl/c-NiO/a-IZO/glass], noted as ECD1 for Y = 600 and ECD2 for Y = 900, where a-WO3 and c-NiO stand for amorphous tungsten oxide and crystalline nickel oxide, respectively. At 555 nm the ECD1 device exhibited the highest coloration efficiency for coloring (CEin = −420.621 cm2·C−1), the highest optical density va... [more]
32743. LAPSE:2023.6709
Knockout of the Aldehyde Dehydrogenase Gene in Fusarium oxysporum for Enhanced Ethanol Yield
February 24, 2023 (v1)
Subject: Biosystems
Keywords: acetic acid, aldehyde dehydrogenase, Fusarium oxysporum, gene knockout
Acetic acid is the primary by-product generated from ethanol production by Fusarium oxysporum using glucose or xylose as a substrate. Aldehyde dehydrogenase (ALDH) is the critical enzyme in acetic acid metabolism. To decrease acetic acid yield in ethanol production, the 1509 bp DNA of aldh, encoding a 502 amino acid protein with a calculated molecular mass of 54.33 kDa and an isoelectric point of 6.21, was cloned from F. oxysporum. Sequence analysis confirmed that the screened proteins belonged to the ALDH family. A knockout vector, ∆aldh, containing positive (hygromycin resistance gene) and negative (thymidine kinase gene from the herpes simplex virus) selectable markers, was constructed. Ethanol production by the mutant (cs28pCAM-Pstal-∆aldh) in glucose- and xylose-containing media was 0.46 and 0.39 g/g, respectively, and these yields were 16.93% and 34.63% higher than those by the wild-type strain (0.393 and 0.289 g/g). Furthermore, the acetic acid yield of the mutant was 3.50 and 3... [more]
32744. LAPSE:2023.6708
Novel Materials and Processes for Photovoltaic Technology
February 24, 2023 (v1)
Subject: Materials
Photovoltaic (PV) technology is the symbol of a sustainable future in many countries around the globe [...]
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