The genus Ocimum has many species that are used to treat diverse kinds of illnesses and sicknesses from ancient times. One of them, Ocimum basilicum L., commonly known as basil, has a vital role due to its various medicinal goods. It is best known as a plant with pharmacological activities, but also as an antioxidant, antimicrobial, and larvicidal agent. Although it has been traditionally used in Serbia in traditional medicine for centuries as an insecticidal, antibacterial, and antifungal plant as well as a traditional culinary plant, none of the O. basilicum varieties have been commercialised until today. There are significant numbers of information across the world that oils and by-products are part of the global market, but no references to the essential oil composition of Serbian plants were found. Therefore, the objective of this work was to evaluate the antioxidant and antimicrobial potentials of essential oil and hydrolate of two different varieties: O. basilicum var. genovese... [more]

Background: In maxillofacial surgery, critical size mandibular defects remain a challenging issue. There have been numerous attempts to improve mandibular defect healing. Recently, bone tissue engineering has provided many benefits in improving bone healing. Herein, we tried to investigate the effect of Mineralized plasmatic matrix (MPM) and Chitosan to enhance tissue healing and regeneration in mandibular bone defect. Methods: A mandibular bone defect of critical size was created in 45 New Zealand rabbits. There were three groups of rabbits: the MPM group, the Chitosan group, and the control group. Radiographical, histological, and immune histochemical evaluations were performed at 4, 8, and 12 post-operative weeks. Results: The MPM group demonstrated the highest degree of bone formation with uniform radio-opacity nearly like that of adjacent healthy parent tissue. While in the chitosan group, most of the defect area was filled with radio-opaque bone with persistent small radiolucent... [more]

The poly(o-anisidine)/BaSO4 nanocomposites were prepared by oxidative polymerization of o-anisidine monomer with BaSO4 filler for the potential antibacterial properties of the composite materials. To achieve the optimal and tunable properties of the nanocomposites, the ratio of BaSO4 filler was changed at the rates of 1%, 3%, 5%, 7%, and 10% with respect to matrix. Different analytical techniques, i.e., FTIR and UV-visible spectroscopy, were employed for functional identification and optical absorption of the poly(o-anisidine)/BaSO4 nanocomposites. The FTIR data revealed the significant interaction between POA and BaSO4, as well as the good absorption behavior of the UV-visible spectra. The conducting properties were controllable by varying the load percentage of the BaSO4 filler. Furthermore, different bacterial strains, i.e., Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive), were used to evaluate the antibacterial activity of the POA/BaSO4 nanocomposit... [more]

Modification of rice protein by extrusion technology can broaden the range of processing and applications for food and feed raw materials. In this study, rice protein was extruded at different screw speeds (100−250 rpm), extrusion temperatures (90−150 °C), and moisture contents (25−40%). Compared with an unextruded protein, the functional properties and structural properties of textured rice protein were evaluated. The results showed that, after extrusion, the solubility of protein was improved, by up to 19.76%, which was 45.23% higher than pre-extrusion; the water holding capacity of extruded rice protein was highest at 200 rpm, 130 °C, and 25%, which could be enhanced by 37.74%; the emulsion stability was enhanced by 152.82% at 200 rpm, 130 °C, and 35%. Under extrusion, the content of sulfhydryl and disulfide bonds of rice protein decreased significantly; the hydrogen bond content increased, and the ionic bond content decreased; the hydrophobic effect decrease, except at 200 rpm, 130... [more]

The development of drug resistance to presently available synthetic medicines leads us to investigate naturally produced small bioactive molecules to treat drug-resistant diseases, such as cancer and other diseases [...]

is used to treat alcoholic liver injury in China; however, its pharmacological mechanism remains to be clarified. Here, the potential of G. uralensis for the treatment of alcoholic liver injury was explored using a network pharmacology and molecular docking approach. The effective components and targets of G. uralensis were searched in a traditional Chinese medicine systems pharmacology database and analysis platform. Disease targets were obtained using the GeneCards and OMIM databases. The target genes of G. uralensis and alcoholic liver injury were compared to obtain common target genes. Symbol conversion was carried out using the Uniport database, and the composition−target network of G. uralensis and alcoholic liver injury was prepared. A protein−protein interaction network was constructed. Gene Ontology functional enrichment and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed. AutoDock was used for the molecular docking of core compounds and... [more]

is an edible mushroom growing in nutritionally poor and desert soil. There has been little information available about its edaphic and culturing conditions for achieving the vigorous mycelial growth essential for its artificial cultivation, bioaugmentation and biodegradation in unfertile soil. Thus, the present study was conducted to assess its edaphic conditions and find a suitable culturing medium for obtaining maximum growth. It grows commonly in coastal soil with saline conditions, barren land soil unfit for cultivation, and desert soil. It forms a basidiocarp singly around xerophytic trees and annual plants and also in soil without vegetation. In addition to a well-developed pileus and stipe, it has a typical rhizoid that grows horizontally in soil. The rhizoid was thick at the base of the stipe and became thin into the mycelial strand. In our earlier study, we reported that its mycelial growth was very poor on nutrient-rich media containing simple sugar, for example, glucose. In... [more]

The process of microbial spray-drying has inherent defects such as short time, complexity, and non-visualization of particle trajectory. However, there has been a lack of effective methods for real-time sampling, rehydration, and non-destructive storage of dried particles, as well as mathematical modeling of the drying process of yeast particles based on sampling and measurement data. Therefore, firstly, a real-time sampling system was developed which completed real-time sampling, rehydration, and non-destructive storage of spray-dried particles, and realized the real reproduction of the changes of yeast particles in the process of spray drying. The laws that the motion trajectory of microbial particles during spray drying are divided into the first cycle region and the reflux cycle region were concluded, and the partition mechanism was explored. Then, based on the sampling data and the law of heat and mass transfer, a mathematical model of porous media was established to predict the v... [more]

plants are of great interest to traditional medicine in the history of several civilizations, particularly in terms of chronic human diseases. Essential oils obtained from this genus have also been used as therapeutic agents. In this present work, two plants of the Ocimum species from Djibouti, Ocimum basilicum L. and Ocimum americanum L., were subjected to hydrodistillation to obtain their essential oils. Gas chromatography-mass spectrometry was performed to determine the chemical composition of both essential oils. Linalool (41.2%) and estragole (30.1%) are the major compounds among the 37 compounds that have been identified in the essential oil of Ocimum basilicum L. (EOOB), and carvotanacetol (38.4%) and estragole (27.5%) are the main compounds among the 42 compounds that have been identified in the essential oil of Ocimum americanum L. (EOOA). Morever, the cytotoxic activity of EOs was evaluated against 13 human cancer cell lines (K562, A549, HCT116, PC3, U87-MG, MIA-Paca2, HEK293... [more]

Cell aggregates have been applied in various fields such as regenerative medicine and drug toxicity testing. We have shown that H-(Lys-Pro)12-OH (KP24), a repeating sequence of lysine (Lys) and proline (Pro), induces uniformly sized cell aggregates simply by its presence in cell suspension. In this study, we considered that this peptide could be applied to a three-dimensional culture substrate that can induce uniform cell aggregates by immobilizing it on the substrate. Therefore, mouse fibroblasts (L929) were seeded on KP24-immobilized glass substrates and cell behavior was observed. We also seeded human-derived cells, namely, human mesenchymal stem cells (hMSC), on KP24-immobilized substrates and characterized their cell assemblies. Furthermore, KP24 was chemically immobilized on the substrate surface, which allowed us to trace the mechanism of KP24−cell interaction. As a mechanism analysis of the cell aggregation ability of KP24, we investigated whether KP24 interacts with the cell s... [more]

The demand for effective, low-cost vaccines increases research in next-generation biomanufacturing platforms and the study of new vaccine delivery systems (e.g., mucosal vaccines). Applied biotechnology in antigen production guides research toward developing genetic modification techniques in different biological models to achieve the expression of heterologous proteins. These studies are based on various transformation protocols, applied in prokaryotic systems such as Escherichia coli to eukaryotic models such as yeasts, insect cell cultures, animals, and plants, including a particular type of photosynthetic organisms: microalgae, demonstrating the feasibility of recombinant protein expression in these biological models. Microalgae are one of the recombinant protein expression models with the most significant potential and studies in the last decade. Unicellular photosynthetic organisms are widely diverse with biological and growth-specific characteristics. Some examples of the specie... [more]

Our research work examined the potential protection of Stevia rebaudiana extract against monosodium urate crystals (MSU)-induced acute gouty arthritis in a rat model and its possible underlying mechanism. Forty rats were allocated into four groups (n = 10); a control group; an MSU group, whose rats received 0.1 of MSU single intra-articular injection in the ankle joint on the fifth day of the experiment; an MSU + Stevia group, which received 250 mg/kg/day of Stevia extract orally for seven days and MSU crystals on the fifth day; and an MSU + colchicine group, which was administered colchicine at 0.28 mg/kg daily for seven days and MSU crystals on the fifth day. Pretreatment with Stevia extract mitigated MSU-induced inflammation as evidenced by a decrease of the ankle edema and inflammatory cell infiltration and a significant downregulation of the protein level of NFκB, TNFα, IL-1β, IL6, and IL18 as well as NLRP3 gene expression. Additionally, there was a markedly increased PPARγ gene e... [more]

The treatment of phenolic-containing wastewater has received increased attention in recent years. In this study, the N-doped porous carbons were prepared from lignite with tripolycyanamide as the N source, and their phenol adsorption behaviors were investigated. Results clearly showed that the addition of tripolycyanamide largely improved the surface area, micropore volume, N content and thus the phenol adsorption capacity of lignite-based carbons. The N-doped sample prepared at 700 °C showed a surface area of 1630 m2/g and a phenol adsorption capacity as high as 182.4 mg/g at 20 °C, which were 2.0 and 1.6 times that of the lignite-based carbon without N-doping. Pseudo-second order and Freundlich adsorption isotherm models could better explain the phenol adsorption behaviors over lignite-based N-doped porous carbon. Theoretical calculations demonstrated that phenol adsorption energies over graphitic-N (−72 kJ/mol) and pyrrolic-N (−74 kJ/mol) groups were slightly lower than that over th... [more]

(Cinnamomum genus) essential oil is commonly used in food preparation and traditional medicines, with a broad spectrum of biological activities. However, research on the extraction of essential oils (EOs) from C. verum cultivated locally in Vietnam is currently limited. Therefore, in this study, the chemical compositions and bioactivities of EOs from the bark and leaves of C. verum collected from the Thai Nguyen and Yen Bai provinces of Vietnam were investigated. The EOs samples were extracted by using water distillation, organic solvent (n-hexane) and ultrasound-assisted (in n-hexane solvent) extraction methods. The chemical composition of the obtained EOs were analyzed by GC-FID and GC/MS analyses. Results showed that the major chemical compositions of C. verum EOs were: (E)-cinnamaldehyde, trans-cinnamic acid, cinnamyl acetate, and benzaldehyde. Furthermore, C. verum EOs exhibited inhibitory activities against two tested cancer cell lines and four bacterial strains. These findings p... [more]

Although immunotherapy has become the standard of treatment for non-small cell lung cancer (NSCLC), only a limited number of patients benefit from it clinically because of the resistance to immunotherapeutic agents. Of late, the usefulness of herbal medicines in combination with immunotherapy has been investigated. Bojungikki-Tang (BJIKT) is a widely used traditional herbal medicine. It synergistically enhances the antitumor effects of chemotherapy and regulates the immune responses in cancer, but its antitumor effect with immunotherapy in NSCLC is unclear. In this study, we investigated the combined effects of BJIKT and an anti-PD-1 antibody in a KLN205-DBA/2 syngeneic lung cancer model. Immunohistochemistry and flow cytometry analyses were performed to analyze the changes in immune cells in the tumor microenvironment. BJIKT plus an anti-PD-1 antibody treatment significantly inhibited tumor growth, unlike the respective monotherapies. Compared to monotherapy, the combination treatment... [more]

Esophageal cancer (ESCA) is one of the highest lethal malignancy tumors worldwide. Baculoviral IAP repeat-containing protein 3 (BIRC3) is the main inhibitor of apoptosis in many malignancies. The aim of this study was to clarify how BIRC3 acts in ESCA cells. Through TNMplot and GEPIA2 analysis, BIRC3 was found abundantly expressed in ESCA cells. The quantitative RT-PCR assay confirmed BIRC3 was pronouncedly induced in all used ESCA cell lines. In addition, proinflammatory cytokines TNFα and IL-1β were shown to have promotion effects on BIRC3 expression in ESCA cells. These promotive effects were blocked when the function of NF-κB was inhibited by bay 11-7082, which indicates the expression of the BIRC3 gene was regulated via the NF-κB transcription pathway in ESCA. Moreover, bioinformatics analysis showed that the BIRC3 gene had many NF-κB binding cis-elements. Chromatin immunoprecipitation was then performed and it was found that NF-κB directly interacts with cis-elements of the... [more]

The combined disinfection process of ultraviolet and sodium hypochlorite has more advantages than the single disinfection method in reducing the disinfectant dosage, shortening the reaction time, and resisting the impact of water quality changes and inhibiting the light reactivation of microorganisms. Given this, using the secondary effluent of a sewage plant as the research object, the disinfection efficiency of the combined process of ultraviolet and sodium hypochlorite was investigated. The experimental results showed that the inactivation effect of UV followed by sodium hypochlorite on fecal coliform and the inhibition of microbial photoreactivation was more significant than that of simultaneous disinfection of UV and sodium hypochlorite disinfection. When the UV dose was 24 mJ/cm2, after disinfection with UV followed by sodium hypochlorite, only 1 mg/L of sodium hypochlorite was required to be added, and a contact reaction time of 1 min for the fecal coliform index to meet the fir... [more]

: The use of herbal extracts could represent an advantageous approach for treating sleeping disorders, especially in mild-to-moderate conditions, before the onset of a specific therapy with first-line drugs. Specifically, the focus was posed about the use of extracts from Valeriana officinalis, Ziziphus jujuba, and Humulus lupulus. Multiple studies demonstrated the efficacy of these medicinal plants to positively manage insomnia symptoms. Additionally, their efficacy in the treatment of sleeping disorders could also be improved by their pharmacological association. In the present study, extracts from Valeriana officinalis, Ziziphus jujuba, Humulus lupulus, melatonin, and their pharmacological association, Vagonotte® MEL, were studied for potential application in the treatment of insomnia. Methods: The extracts and melatonin were tested on hypothalamic neurons and tissue for evaluating biocompatibility and protective and neuromodulatory effects. The neuromodulatory effects were evaluate... [more]

This study aimed to investigate the chemical composition of steam distillate essential oil and corresponding hydrolate obtained from S. officinalis grown in Serbia, as well as the influence of weather conditions (temperature and precipitations) on their chemical profiles. Furthermore, their antimicrobial activity was investigated in vitro. The main compounds in essential oil were cis-thujone, followed by camphor, trans-thujone, and 1,8-cineole, while hydrolate was slightly different from the essential oil, with camphor, cis-thujone, and 1,8-cineole as the main compounds. Among the eight respiratory-associated microorganisms, Klebsiella oxytoca was the most sensitive to the tested EOs (minimum inhibitory concentration (MIC)/minimal bactericidal/fungicidal concentration (MBC/MFC) were 14.20 and 28.4 μL mL−1, respectively). MIC and MBC values of other tested bacteria ranged between 28.40 and 227.25 μL mL−1 while for Candida albicans MIC/MFC ranged from 28.40/56.81 to 56.81−113.63 μL mL−1.... [more]

Monitoring and controlling stability in anaerobic digestion (AD) systems are essential, since it allows to obtain information that helps to take corrective actions in case of deviations in the system and to guarantee a stable performance in the biogas production. In this work, a pilot-scale CSRT reactor (1 m3) was monitored during the anaerobic digestion of pig manure with thermal pretreatment (80 °C) operated at thermophilic temperature (45 °C). The ratio of the volatile organic acids (FOS) to the total inorganic carbonate (TAC) and the pH were the indicators used during the monitoring process to identify deviations in the AD system. Additionally, alkaline solution NaOH (98%) was applied to counteract pH deviations and maintain stability. Chemical oxygen demand (COD) and biogas composition were measured during the AD process. It was found that during the AD process, the FOS/TAC was between the range of 0.5 and 1. The results revealed that, in the anaerobic digestion of pig manure with... [more]

Background: The effect of cannabis on cholesterol and lipid balance has been reported for decades. However, there are conflicting reports on the reduction of low-density lipoprotein (LDL-C) and total cholesterol. The purpose of this study was to determine the immediate changes of Cannabis spp. consumption by pyrolytic route in heavy users. Methods: A cross-sectional study on 20 Peruvian heavy cannabis users (mean age: 31 ± 9.5 years). The inclusion criteria were males with an average weight of 50−70 kg, normal BMI, and having used cannabis, without association with other drugs, for at least one year with a high frequency per week (use: 4−7 days/week). High-density lipoprotein (HDL-C), LDL-C, and total cholesterol were evaluated 30 and 120 min after the administration of Cannabis spp. (~0.2 g by inhalation). Results: Of the total 12 (60%), 10 (50%), and 11 (55%) had desirable total cholesterol, fairly good HDL-C (40−60 mg/dL) and fairly good LDL-C (100−129 mg/dL) values, respectively. T... [more]

Vitamin C, C6H8O6, is a water-soluble vitamin that is widespread in nature. It is an essential nutrient involved in many biological processes in the living organisms: it enhances collagen biosynthesis, ensures the optimal functioning of enzymes and the immune system, has a major role in lipid and iron metabolism, and it enhances the biosynthesis of l-carnitine. Due to its antioxidant activity, vitamin C can neutralize the tissue-damaging effects of free radicals. Vitamin C is being related to the prevention of cancer and cardiovascular diseases. This review includes current information on the biosynthesis of ascorbic acid, as new methods are now challenging the traditional Reichstein process for vitamin C’s industrial-scale production. Different strains were analyzed in correlation with their ability to synthesize ascorbic acid, and several separation techniques were investigated for a more effective production of vitamin C.

Wastewater disinfection is one of the most critical issues in protecting human health against exposure to waterborne pathogenies. Chlorine is among the most commonly used disinfectants in many wastewaters’ treatment plants. Nevertheless, disquiets regarding chlorine’s disinfection by-products (DBPs) have grown recently. One of the most effective ways to reduce DBPs generation is to reduce chlorine dosage by increasing disinfectant efficiency. Using magnetic field (MF) in wastewater treatment is one of the promising research topics with significant progression. This study aimed to evaluate the efficiency of using a magnetic field and/or sodium hypochlorite (NaClO) disinfection on secondary slaughterhouse wastewater effluent quality and by-products. Three groups of secondary slaughterhouse wastewater effluents were used: G1 was treated with NaClO only at 0, 2, 4, and 6 mg/L; G2 was treated with exposure to MF at 14,500 gausses, and G3 was pretreated with MF, then NaClO at the exact chlor... [more]

Attached microalgal growth of Chlorella vulgaris on palm decanter cake (PDC) under irradiation with various visible monochromatic and polychromatic spectra to produce biodiesel was studied in this work. The results demonstrated that the white spectrum cultivation exhibited the highest microalgal density of 1.13 g/g along with 1.213 g/L day of microalgal productivity. Correspondingly, the biodiesel obtained was comprised mainly of C16 and C18 fatty acids, possessing a high cetane number and oxidation stability from the high saturated fatty acid content (70.38%), which was appealing in terms of most biodiesel production requirements. Nevertheless, the highest lipid content (14.341%) and lipid productivity (93.428 mg/L per day) were discovered with green spectrum cultivation. Blue and white spectra led to similar protein contents (34%) as well as carbohydrate contents (61%), corroborating PDC as a feasible carbon and nutrient source for growing microalgae. Lastly, the energy feasibilities... [more]

The imbalance in the expression of matrix metalloproteinases (MMPs) and lysyl oxidases (LOXs) in synovial fibroblasts (SFs) caused by mechanical injury and inflammatory response prevents injured anterior cruciate ligaments (ACLs) from self-healing. However, research on the effect of growth factors on SFs on regulating the microenvironment is limited. In this study, mechanical injury and exogenous transform growth factor-β1 (TGF-β1) were employed to mimic a joint-cavity microenvironment with ACL trauma. The function of the NF-κB transcription factor was further studied. The study found that the gene expression of LOXs (except LOXL-1), MMP-1, -2, and -3 in SFs was promoted by the combination of injurious mechanical stretching and TGF-β1 and that the upregulation of MMPs was higher than that of LOXs. In addition, MMP-2 activity induced by the combination of injurious stretch and TGF-β1 was inhibited by NF-κB inhibitors such as Bay11-7082 and Bay11-7085. The findings concluded that the syn... [more]