Ciprofloxacin (CIP), as a common antibiotic used in human clinical and livestock farming, is discharged into natural water bodies and its concentration has increased in the last years. Its stable chemical structure is difficult to remove by conventional techniques. Residual ciprofloxacin in the environment has become an emerging micropollutant that promotes the generation of resistance genes of bacteria and endangers ecosystem balance and human health. Removal of ciprofloxacin from water by the system of ultrasound/electric field/sodium persulfate (US/E/PS) was investigated. Firstly, CIP degradation affects by different oxidation methods, such as ultrasonic oxidation, electro-oxidation, and persulfate oxidation, and their four combined oxidation methods (ultrasound-activated persulfate oxidation, electro-activated persulfate oxidation, ultrasound-enhanced electro-oxidation, and ultrasound-enhanced electro-activated persulfate oxidation), on the target contaminants were compared. Second... [more]

Recent discoveries have shown that calcium carbonate and soy protein interactions can be used to reinforce rubber composites with improvements on the effective crosslink density and moduli. However, the method to incorporate the soy protein into the rubber matrix may be costly to scale up, since it involves microfluidization and drying steps prior to rubber compounding. In this work, a simpler process involving dry-milled calcium carbonate and soy protein was used to explore filler blends of calcium carbonate, soy protein, biochar, and carbon black. By blending these filler materials in various ratios, rubber composite samples with 40−50% of the carbon black replaced by sustainable alternatives were made. These composites had essentially the same tensile strength, with better toughness and elongation properties relative to the carbon black control. These composites would reduce dependence on petroleum and be more amenable to the rubber composite compounding infrastructure.

Molecular dynamics (MD) simulation is a particularly useful technique in food processing. Normally, food processing techniques can be optimized to favor the creation of higher-quality, safer, more functional, and more nutritionally valuable food products. Modeling food processes through the application of MD simulations, namely, the Groningen Machine for Chemical Simulations (GROMACS) software package, is helpful in achieving a better understanding of the structural changes occurring at the molecular level to the biomolecules present in food products during processing. MD simulations can be applied to define the optimal processing conditions required for a given food product to achieve a desired function or state. This review presents the development history of MD simulations, provides an in-depth explanation of the concept and mechanisms employed through the running of a GROMACS simulation, and outlines certain recent applications of GROMACS MD simulations in the food industry for the... [more]

The release of winery wastewater (WW) into the environment, without proper treatment, can cause severe problems to freshwater quality and natural fauna and flora. Therefore, in this work a treatment process was studied, combining adsorption and thermocatalytic oxidation processes. In a more specific way, it optimized the combination of activated sodium bentonite (Na-Mt) and potassium persulfate (KPS)/sodium percarbonate (SPC) as oxidant agents. With the combination of best operational conditions of adsorption ([Na-Mt] = 5.0 g/L, pH = 3.0, V = 500 mL, agitation 350 rpm, T = 298 K, t = 24 h) and thermocatalytic oxidation processes (S2O82−/H2O2 ratio = 1:0.25, S2O82−/H2O2 dosage = 0.1:0.025 (g/g), pH = 7.0, T = 343 K, agitation 350 rpm, t = 2 h), a total organic carbon, chemical oxygen demand and total polyphenols removal of 76.7, 81.4 and >99% was achieved, respectively. Finally, it was evaluated the effect of the treatment processes in the germination index (GI) of different plant seeds... [more]

Acetone released into the atmosphere can adversely affect human health and the environment. The aim of this work was to evaluate the performance of a laboratory-scale biotrickling filter (BTF) with bioball packing material to remove acetone vapor from contaminated air. The acetone removal efficiency was investigated in two different scenarios: with and without the inoculation of microorganisms. Three strains of bacteria, Pseudomonas putida, Rhodococcus aerolatus, and Aquaspirillum annulus, were used in the BTF. In both cases, the filter units were simultaneously operated for 100 days under three different inlet acetone concentrations (0.18 ± 0.01 g/m3, 0.25 ± 0.01 g/m3, and 0.40 ± 0.02 g/m3) and two different gas flow rates (2.54 and 5.09 m3/h). The results showed that acetone removal was greater in the filter with the inoculated bacteria. In the filter operated without inoculum, the acetone removal efficiency gradually decreased with filtration time from 90.1% to 6.1%. While employing... [more]

The rising trend in the consumption of healthy, safe, and functional foods has motivated studies on cold-pressed specialty oils, including macadamia nut oil. Cold-pressed macadamia nut oil (CPMO) is given preference by consumers over solvent extracted and refined oil because of its exceptional quality attributes and safety. This review contains a detailed presentation of the chemical properties, health benefits, and applications of CPMO. The monounsaturated fatty acids (oleic acid and palmitoleic acid) rich oil also contains a significant concentration of bioactive phytochemicals including, β-sitosterol, α-tocopherol, α-tocotrienols, ρ-hydroxybenzoic acid, and caffeic acid. Moreover, the oil has good oxidative stability. The highlighted properties offer CPMO health benefits related to the prevention of cardiovascular diseases, diabetes, cancer, high blood pressure, and neurodegenerative diseases. The fatty acid composition of CPMO allows for its diverse application in the food, cosmeti... [more]

Maqui (Aristotelia chilensis (Mol.) Stuntz) is a Chilean berry rich in antioxidants, which are mostly found in the pulp and skin of the fruit. The objective was to evaluate the cryoconcentration process by centrifugation−filtration as a simultaneous, efficient, and innovative method to increase the content of thermosensitive bioactive compounds of aqueous maqui extract. Cryoconcentration separated the concentrated solute from the aqueous maqui extract with an efficiency of more than 95%; it increased the content of total polyphenols and total anthocyanins and antioxidant capacity by 280%, 573%, and 226%, respectively. Although the concentrates obtained by evaporation at 50, 70, and 80 °C increased the content of bioactive compounds, they did so in a lower percentage than the cryoconcentrate. Furthermore, cyanidin 3,5-diglucoside was degraded at 70 and 80 °C. In conclusion, cryoconcentration by centrifugation−filtration as a simultaneous process efficiently separates the solutes from th... [more]

The mechanisms involved in immune responses to cancer have been extensively studied for several decades, and considerable attention has been paid to harnessing the immune system’s therapeutic potential. Cancer immunotherapy has established itself as a promising new treatment option for a variety of cancer types. Various strategies including cancer vaccines, monoclonal antibodies (mAbs), adoptive T-cell cancer therapy and CAR T-cell therapy have gained prominence through immunotherapy. However, the full potential of cancer immunotherapy remains to be accomplished. In spite of having startling aspects, cancer immunotherapies have some difficulties including the inability to effectively target cancer antigens and the abnormalities in patients’ responses. With the advancement in technology, this system has changed the genome-based immunotherapy process in the human body including the generation of engineered T cells. Due to its high specificity, CRISPR-Cas9 has become a simple and flexible... [more]

Biogenic amines (BAs) are considered a potential microbiological toxicological hazard in aged cheese. Risk mitigation strategies include good hygiene practice measures, thermal treatment of milk and the use of competitive dairy cultures. The aim of this study was to evaluate the amount of BAs—tryptamine, β-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine—in the core and rind of cheeses ripened by bacteria (n = 61) and by mold cultures (n = 8). The microbial communities were counted, and the dominant lactic acid bacteria (LAB) were identified, corresponding to the BA concentrations. The total BA content was highest in the core of semi-hard cheeses (353.98 mg/kg), followed by mold cheeses (248.99 mg/kg) and lowest in hard cheeses (157.38 mg/kg). The highest amount of BAs was present in the rind of cheeses with mold (240.52 mg/kg), followed by semi-hard (174.99 mg/kg) and hard cheeses (107.21 mg/kg). Tyramine was the most abundant BA, represented by 7... [more]

The study presents a comparative investigation of the composition and bioactivity of oil extracted from pomegranate seeds of sun-burned fruit (SB) and healthy fruit (HF) for the value-adding potential of pomegranate fruit waste. Seeds from SB and HF were independently freeze dried, sun dried, and oven dried before ultrasound-assisted oil extraction using petroleum ether. The extracted oil was analysed for yield, refractive index, ρ-anisidine value, total phenolic content (TPC), DPPH radical scavenging ability, antimicrobial activity, tyrosinase enzyme inhibition ability, and fatty acid composition. The results showed that oven dried seeds, regardless of fruit quality, yielded the highest oil (20.85−24.70%, dry weight). Regardless of the seed drying method, oil from the seeds of SB exhibited the highest TPC (1.48−2.84 mgGAE/g PSO) than oil from the seeds of HF. The oil from oven dried and freeze dried seeds of SB were more effective in scavenging the DPPH radicals with IC50 values of 34... [more]

This study aimed to evaluate the effects of partial substitution of sodium chloride (NaCl) with potassium chloride (KCl) in combination with high-pressure processing (HPP) on the physicochemical properties and volatile compounds of beef sausage during cold storage at 4 °C. Significant differences were found in the volatile compounds of beef sausages with 0%, 25%, and 50% NaCl contents partially substituted with KCl subjected to 28 days of storage and were well-visualized by heat map analysis. A total of 75 volatile compounds were identified and quantified in the beef sausages at the end of 28 days of storage, including 12 aldehydes, 4 phenols, 2 ketones, 18 alcohols, 8 acids, 3 esters, 14 terpenes, and 14 alkanes. Thirteen compounds had low odor activity values (OAV) (OAV < 1); however, high OAV (OAV > 1) were obtained after partial substitution of NaCl by KCl at 25% and 50% with HPP treatment compared to the non-HPP treated samples. In addition, 50% NaCl substitution with KCl in conju... [more]

Breast cancer (BC) is one of the most common malignancies in women. Although widespread successful synthetic drugs are available, natural compounds can also be considered as significant anticancer agents for treating BC. Some natural compounds have similar effects as synthetic drugs with fewer side effects on normal cells. Therefore, we aimed to unravel and analyze several molecular mechanisms of genistein (GNT) against BC. GNT is a type of dietary phytoestrogen included in the flavonoid group with a similar structure to estrogen that might provide a strong alternative and complementary medicine to existing chemotherapeutic drugs. Previous research reported that GNT could target the estrogen receptor (ER) human epidermal growth factor receptor-2 (HER2) and several signaling molecules against multiple BC cell lines and sensitize cancer cell lines to this compound when used at an optimal inhibitory concentration. More specifically, GNT mediates the anticancer mechanism through apoptosis... [more]

Pyraclostrobin is a fungicide extensively used for the control of various fungal diseases and is frequently detected in environmental samples. Natural systems, such as constructed wetlands (CWs) and gravity filters, are effective and environmentally friendly treatment systems, which can reduce or eliminate pesticides from the environment. The aim of this study was to investigate the capacity of two pilot-scale CWs (porous media: cobbles and fine gravel, planted with Phragmites australis) and six gravity filters (filling material: bauxite, carbonate gravel and zeolite) to remove pyraclostrobin from polluted water originating from spraying equipment rinsing sites. For this, experiments were conducted to test the performance of the above natural systems in removing this fungicide. The results showed that the mean percent pyraclostrobin removal efficiencies for cobbles and fine gravel CW units were 56.7% and 75.2%, respectively, and the mean percent removals for HRTs of 6 and 8 days were 6... [more]

Artificial sweeteners are food additives worldwide used instead of fructose or glucose in many diet beverages. Furthermore, diet beverages intake has been increasing every year. Thus, some food agencies should regulate it based on toxicological studies. Debates and controversial results are demonstrated, and authority can revise its decision on the basis of new data reporting toxicological effects since cyclamate has been forbidden in some countries. Therefore, the aim of this study was to report new data about the toxicity of acesulfame-k, aspartame, and cyclamate, which are useful for authority agencies, determining the toxic potential and nutraceutical capabilities of these compounds. The toxicity, antitoxicity, genotoxicity, antigenotoxicity, and life expectancy assays were carried out in Drosophila as an in vivo model. In addition, in vitro HL-60 line cell was used to evaluate the chemopreventive activity determining the cytotoxic effect and the capability of producing DNA damage... [more]

Dynamics of the microbiological indicators of fresh sludge from wastewater treatment plants with a concentration of CaO, 10% and 20%, and ash, −30% and 50%, and treated with quicklime, ash, and microbial fertilizer for a 50-day period of composting were studied. The influence of temperature, water content, and oxygen on the development of microbes was established in laboratory conditions. Microbiological analysis included the determination of non-pathogenic (non-spore-forming bacteria, bacilli, actinomycetes, micromycetes, bacteria digesting mineral nitrogen), and pathogenic (Salmonella, Listeria, Escherichia coli, Enterococcus, Clostridium perfringens) microorganisms. Of the beneficial microflora in the sludge before treating, the amount of non-spore-forming bacteria was the highest, followed by bacilli and micromycetes. It was found that actinomycetes were absent in the untreated sludge. Clostridium perfringens occupied a major share in the composition of the pathogenic microflora, f... [more]

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an advanced technique that uses minimum fragmented ions from complex molecules for mass spectrometry (MS) analysis (tissue profiling by mass spectrometry). It is able to analyze spatially resolved tissue or tumor sections at the molecular level. It has become a valuable tool for tumor and tissue imaging, due to its ease of operation and high mass resolution, but it still has vast room for development in the instrumentation of larger proteins in some tissues. In this review, we focus on the main components of MALDI-MS instrumentation, sample handling and processing, the working principle of MALDI-MS, and its applications in diagnostic and prognostic assessments, tumor removal and drug development. Although it is less effective at detecting larger proteins in some tissues, it still shows huge potential because of its advancements in instrumentation and processing protocols. This article may benefit those who have... [more]

To identify effective ways of increasing the yield of crops grown in nutrient-poor calcareous soils, the combined effects of biochar addition and inoculation with plant growth promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) on wheat growth and soil properties were investigated under rhizobox conditions. Measured soil properties included pH, electrical conductivity (EC), organic matter content (OM), the availability of P, Fe, and Zn in the rhizosphere, and the uptake of these elements by plants. Combined biochar addition and microbial inoculation were shown to significantly increase the concentration of available forms of P, Fe, and Zn in the soil when compared to non-biochar treatments. The highest soil pH (7.82) was observed following biochar addition without microbial inoculation. The EC following biochar addition and PGPR inoculation was significantly higher than the other treatments, and the soil OM content was highest when combining AMF inoculation with bioch... [more]

This study aimed at evaluating the impact of seasons on the nutritional status and on dietary cassava-related cyanide exposure in Burhinyi and Idjwi, two areas in the eastern Democratic Republic of the Congo, witnessing similarly high cassava-derived cyanide poisoning but differently affected by konzo and malnutrition. Cyanide content in cassava roots and flour, and urinary thiocyanate levels (uSCN) of 54 subjects (40 from Burhinyi and 14 from Idjwi, aged 28.7 (12.1) years, 63% women) were measured during the rainy season (RS) and dry season (DS), using picrate paper kits A and D1. Local processing methods proved to be efficient in removing cyanogenic compounds in fresh cassava roots during the RS. However, the cyanide content in flour samples significantly increased during DS, with ~50% of samples containing unsafe levels (>10 ppm) of cyanide content. Strikingly, the uSCN (µmol/L), from being comparably high in RS (~172.0), slightly decreased during DS in Burhinyi (~103.2; p = 0,3547)... [more]

(PGCAM) is a well-known phytomedicine, but most of its compounds, such as ginsenoside derivatives, have poor absorption and bioavailability profile due to high molecular weight (≥500 Daltons), which is the major hurdle for their clinical application. Hence, this research explored the efficiency of low molecular weight compounds (LMWCs) (<500 Daltons) screened from PGCAM and their anti-COVID-19 mechanisms through network pharmacology. Molecular compounds from PGCAM were identified using public databases and filtered out by the drug-likeness evaluation. Genes interacted with these filtered compounds, and COVID-19-related genes were extracted from public databases. In addition, overlapping genes between compounds and interactive genes were identified using the Venn diagram. In parallel, the networking between compounds and overlapping genes was analyzed by RStudio. The pathway enrichment analysis of overlapping genes was determined by STRING. Finally, the key bioactive compounds were d... [more]

Nowadays, a general interest in improving health in order to achieve better conditions of life is increasing. Diet is a complex factor affecting health conditions. We analysed the biological activities of three types of alcohol-free lager beer (a blond, a pale-blond and a stout beer) as well as epicatechin gallate (ECG) as one of their most abundant phenols with the aim of revealing them as nutraceuticals. For that purpose, we carried out safety and protective assays of the tested substances in the well-known Drosophila melanogaster animal model. Moreover, chemoprevention studies on human leukaemia cells (HL-60) in an in vitro model were carried out to evaluate the viability and genomic damage potential of the studied compounds on the tumour cell line. Results suggest the safety properties of all compounds, although pale-blond and stout beer only showed genotoxic activity at the lowest concentrations assayed. Moreover, alcohol-free beers and phenols were able to protect against H2O2 ox... [more]

The coronavirus disease 19 (COVID-19) is a global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has a rapidly increasing prevalence and has caused significant morbidity/mortality. Despite the availability of many vaccines that can offer widespread immunization, it is also important to reach effective treatment for COVID-19 patients. However, the development of novel drug therapeutics is usually a time-consuming and costly process, and therefore, repositioning drugs that were previously approved for other purposes could have a major impact on the fight against COVID-19. Here, we first identified lung-specific gene regulatory/interaction subnetworks (COVID-19-related genes modules) enriched for COVID-19-associated genes obtained from GWAS and text mining. We then screened the targets of 220 approved drugs from DrugBank, obtained their drug-induced gene expression profiles in the LINCS database, and constructed lung-specific drug-related gene modul... [more]

In the search for new antimicrobial lead compounds, interest in natural-product-based screening has enjoyed a renaissance, driven by the fact that plants present a unique pool of compounds [...]

Pharmaceutical products that mix natural raw materials are subject to unavoidable contamination with microorganisms from the environment and animals. This study focused on the effect of radiation on the quality of primary packaged pills, which are crude drug products. The pills, which were sealed in a sack for primary packaging laminated with polyethylene terephthalate, polyethylene, and aluminum foil, were irradiated by gamma rays or electron beam (EB). The survival counts of bacteria were reduced to 103 CFU/g or less by 6 kGy of irradiation. The counts of the spore-forming bacteria Bacillus megaterium, B. cereus, and Brevibacillus brevis in the pills were reduced to not over 100 CFU/g after 10 kGy irradiation. Although some of the cinnamaldehyde in the pills was oxidized to cinnamic acid, the decomposition of swertiamarin, berberine, glycyrrhizin, and cinnamaldehyde in the pills after 10 kGy irradiation were within the analytical accuracy by high-performance liquid chromatography. Ga... [more]

Worldwide, Tuberculosis (TB) is caused by Mycobacterium tuberculosis bacteria. Ocimum sanctum, commonly known as holy basil (Tulsi), is an herbaceous perennial that belongs to the family Lamiaceae and is considered one of the most important sources of medicine and drugs for the treatment of various diseases. The presented study aims to discover the potential phenomenon of Ocimum sanctum in the medicament of tuberculosis using a network pharmacology approach. Active ingredients of Ocimum sanctum were fetched through two different databases and from literature review and then targets of these compounds were harvested by SwissTargetPrediction. Potential targets of TB were downloaded from GeneCards and DisGNet databases. After screening of mutual targets, enrichment analysis through DAVID was performed. Protein−protein interaction was performed using the String database and visualized by Cytoscape. Then the target-compound-pathway network was constructed with Cytoscape. In the end, molecul... [more]

Infectious diseases are one of the core biological complications for public health. It is important to recognize the pathogen-specific mechanisms to improve our understanding of infectious diseases. Differentiations between bacterial- and viral-targeted human proteins are important for improving both prognosis and treatment for the patient. Here, we introduce machine learning-based classifiers to discriminate between the two groups of human proteins. We used the sequence, network, and gene ontology features of human proteins. Among different classifiers and features, the deep neural network (DNN) classifier with amino acid composition (AAC), dipeptide composition (DC), and pseudo-amino acid composition (PAAC) (445 features) achieved the best area under the curve (AUC) value (0.939), F1-score (94.9%), and Matthews correlation coefficient (MCC) value (0.81). We found that each of the selected top 100 of the bacteria- and virus-targeted human proteins from a candidate pool of 1618 and 391... [more]