Microbial fuel cell (MFC) technology has captured the scientific community’s attention in recent years owing to its ability to directly transform organic waste into electricity through electrochemical processes. Currently, MFC systems faces a number of barriers, with one of the most significant being the lack of organic substrate to provide enough energy for bacterial growth and activity. In the current work, rotten rice was utilized as an organic substrate to boost bacterial activity to produce more energy and break down the organic pollutant hydroquinone in an effort to improve the performance of MFCs. There are only a few studies that considered the waste as an organic substrate and simultaneously degraded the organic pollutant vis-à-vis MFCs. The oxidation of glucose derived from rotten rice generated electrons that were transported to the anode surface and subsequently flowed through an external circuit to the cathode, where they were used to degrade the organic pollutant hydroqui... [more]

The inhibition of Escherichia coli ATCC 25922 (E. coli), Staphylococcus aureus ATCC6538 (S. aureus), Salmonella Enteritidis ATCC 13076 (S. Enteritidis), and Listeria monocytogenes DSM12464 (L. monocytogenes) is one of the main aims of the food industry. This study was the first in which the use of ultrasound and microwave power were applied to optimize the values of the bioactive components, amino acids, and mineral compositions of tangerine juice and to inhibit Escherichia coli, Staphylococcus aureus, Salmonella Enteritidis, and Listeria monocytogenes. The response surface methodology (RSM) was used to describe the inactivation kinetics, and the effects of ultrasound treatment time (X1: 12−20 min), ultrasound amplitude (X2:60−100%), microwave treatment time (X3: 30−40 s), and microwave power (X4:200−700 W). The optimum parameters applied to a 5-log reduction in E. coli were determined as ultrasound (12 min, 60%) and microwave (34 s, 700 W). The optimum condition ultrasound−microwave t... [more]

As the major byproduct of the winemaking industry, grape pomace remains an untapped source of valuable bioactive phenolic compounds. This study elucidated the optimal aqueous extraction parameters for maximizing phenolic extractability, while avoiding the use of harsh conventional solvents and limiting water usage, from Cabernet Sauvignon grape pomace in which the red grape was processed for white wine. In the single-stage aqueous extraction process (AEP), the concurrent impact of pH (2.64−9.36), solids-to-liquid ratio (SLR, g pomace/mL water) (1:50−1:5), and temperature (41.6−58.4 °C) on the total phenolic content (TPC) of Cabernet Sauvignon pomace was evaluated alongside a kinetic study (15−90 min). Optimal single-stage extraction conditions (pH 9.36, 1:50 SLR, 50 °C, 75 min) guided the development of a two-stage countercurrent extraction process (pH 9.36, 1:10 SLR, 50 °C, 75 min) to further reduce water consumption without compromising overall extractability. The countercurrent proc... [more]

As people around the world regard 2020 as the year of COVID-19, the medical community considers this year to be the second-best year, shared with the year 1996, with respect to the number of drug molecules approved by the US Food and Drug Administration (FDA). Both years, 2020 and 1996, had a record of 53 new drug molecules approved by the FDA. In the year 2020, 53 new chemical entities and 13 biological medicines were approved, including 10 monoclonal antibodies, 2 antibody-drug conjugates, 3 peptides, and 2 oligonucleotides. Among them, most of the compounds were found to have fluorine or fluorine-containing functional groups exhibiting numerous pharmacological activities. Herein, we summarized the trifluoromethyl (TFM, -CF3)-group-containing FDA-approved drugs for the last 20 years. This article specially features and details the previous 20-year literature data, covering CF3-incorporated potential drug molecules, including their syntheses and uses for various diseases and disorders... [more]

Validating ethnobotanical data from underexplored traditional plant remedies provides an infinite source of new pharmaceutical chemicals. The purpose of this study was to determine the phytochemical composition and several biological activities (antioxidant, anti-lipase, anti-α-amylase, anti-α-glucosidase, and antimicrobial) of aqueous, ethanol, hexane, and acetone Artemisia scoparia leaf extracts. An exhaustive technique was employed to extract A. scoparia four extracts. At the same time, standard analytical and biochemical assays were utilized to determine preliminary phytochemical screening, anti-DPPH, anti-lipase, anti-α-glucosidase, and anti-α-amylase activities. Furthermore, the antimicrobial effects against seven microbial strains were evaluated using a broth micro-dilution assay. Acetone A. scoparia extract exhibited the highest DPPH scavenging and anti-α-glucosidase activities (IC50 = 21.87 ± 0.71, and 149.75 ± 1.33 µg/mL, respectively), as well as the ethanol extract, exhibit... [more]

Diabetes mellitus (DM) is a chronic metabolic disorder in which the pancreas does not produce enough insulin or the body cannot effectively use it. The prevalence of diabetes is increasing steadily, making it a global public health problem. Several serious complications are associated with this disease. There are a number of different classes of antidiabetic medications. Interestingly, traditional medicine can also be used for the development of novel classes of hypoglycemic therapeutics. This article summarizes an update of the potential of various important medicinal plants used in the development of nutraceuticals for the management of diabetes mellitus, and a proposal of their biological mechanisms.

Rice bran is a by-product of the rice milling process. It contains a high concentration of protein. Rice brans are frequently utilized as feed cattle, fertilizer, and fuel. However, their application as human nutrition supplements is uncommon, and the necessary process for this purpose is yet to be established, including the drying process. This study aims to evaluate the effect of the spray-drying parameters, the inlet temperature, inlet flowrate, and inlet air flowrate, on rice bran protein (RBP) powder and optimize it using response surface methodology (RSM). A thermal water-based extraction method was utilized prior to the drying process. The correlation between the spray-drying parameters, i.e., the inlet temperature (120 to 210 °C), feed flowrate (5 to 55%), and air flowrate (246 to 670 L/h), and the RBP yield were investigated. The quality of the RBP powder was evaluated based on acid amino profiling in the mixture through de novo peptide sequencing. The optimized operating cond... [more]

The purpose of this study was to evaluate the performance of laboratory-scale biotrickling filters (BTFs) packed with composite filler and pine bark filler under different operating conditions in purifying mixed gas containing H2S and NH3. The composite filler was prepared with modified activated carbon and loaded with functional microbes, using the microbial immobilization technology combined with a nutrient sustained-release composite filler. The results showed that the composite filler could better adapt to low empty bed retention time (EBRT) and high inlet concentration than the pine bark filler. When EBRT was 40 s and the inlet load was 41 g/m3·h, the NH3 removal efficiency of the composite filler was kept above 80%, and when the inlet load was 61.5 g/m3·h, it could be stabilized at about 60%. When EBRT exceeds 34 s, the H2S removal efficiency of the two BTFs was maintained at 100%. Yet, when EBRT was 34 s, the H2S removal efficiency of the bark filler BTF dropped to <80%. The... [more]

Fruit waste contains several bioactive components such as polyphenols, polysaccharides, and numerous other phytochemicals, including pigments. Furthermore, new financial opportunities are created by using fruit ‘leftovers’ as a basis for bioactivities that may serve as new foods or food ingredients, strengthening the circular economy’s properties. From a technical standpoint, organic phenolic substances have become more appealing to industry, in addition to their application as nutritional supplements or functional meals. Several extraction methods for recovering phenolic compounds from fruit waste have already been published, most of which involve using different organic solvents. However, there is a growing demand for eco-friendly and sustainable techniques that result in phenolic-rich extracts with little ecological impact. Utilizing these new and advanced green extraction techniques will reduce the global crisis caused by fruit waste management. Using modern techniques, fruit resid... [more]

As the third most plentiful biopolymer after other lignocellulosic derivates such as cellulose and hemicellulose, lignin carries abundant potential as a substitute for petroleum-based products. However, the efficient, practical, value-added product valorization of lignin remains quite challenging. Although several studies have reviewed the valorization of lignin by microorganisms, this present review covers recent studies on the valorization of lignin by employing yeast to obtain products such as single-cell oils (SCOs), enzymes, and other chemical compounds. The use of yeasts has been found to be suitable for the biological conversion of lignin and might provide new insights for future research to develop a yeast strain for lignin to produce other valuable chemical compounds.

Research on the use of microbial biosensors for monitoring wastewater contaminants is a topic that covers few publications compared to their applicability in other fields, such as biomedical research. For this reason, a systematic analysis of the topic was carried out, for which research-type articles were reviewed during the period 2012 to September 2022. For this, different search platforms were used, including PubMed, ScienceDirect, Springer Link, and Scopus, and through the use of search equations a relevant bibliography was located. After that, the research articles were selected based on exclusion criteria. As a result, it was found that, of the 126 articles, only 16 articles were strictly related to the topic, since there was a duplication of articles among the different databases. It was possible to demonstrate the usefulness of microorganisms as components of biosensors to monitor BOD, heavy metals, and inorganic contaminants in wastewater that also had a high sensitivity. Add... [more]

Methyl isobutyl ketone (MIBK) is a highly valuable product in the chemical industry. It is widely used as an extracting agent for heavy metals, antibiotics, and lubricating oils. Generally, MIBK can be produced by three-step and one-step liquid-phase methods. These methods are expensive and energy-demanding due to the high pressure and low conversion of acetone. A novel nano-Pd/nano-ZnCr2O4 catalyst was developed to produce MIBK with high conversion and selectivity of 77.3% and 72.1%, respectively, at 350 °C and ambient pressure, eliminating the need for high pressure in conventional MIBK processes. This study is the first that proposes a newly developed process of methyl isobutyl ketone (MIBK) production using the low-pressure one-step gas-phase selective hydrogenation of acetone. In this work, a novel process flow diagram has been developed for the production of MIBK using the developed nano-catalyst. The process was heat integrated, resulting in a 26% and a 19.5% reduction in the he... [more]

To study the mechanism of galangal in the treatment of gastric cancer by network pharmacology. The TCMSP database was used to collect the effective compounds and potential targets of galangal, and the genes associated with gastric cancer were obtained through the GeneCards database, and Venn obtained the interaction genes of the effective compound targets of galangal and gastric cancer targets, plotted the interaction genes into PPI networks, and screened out key targets. The interacting genes were imported into Metascape database for GO enrichment analysis and KEGG signal enrichment. A total of 13 active compounds and 207 potential downstream target genes were screened by TCMSP database. Have 5222 gastric cancer target genes through GeneCards database, there were a total of 150 interactive genes and 6 key genes: TP53, AKT1, JUN, HSP90AA1, IL6, and CASP3. These interacting genes involved 30 typical GO entries and 20 KEGG signals. Galangal may play a role in the treatment of gastric can... [more]

The biosorption of arsenic (As) with macroalgae has aroused much interest as a clean and low-cost technology. To evaluate arsenic biosorption by Chondracanthus chamissoi and Cladophora sp., approximately 5 kg of algae was collected from Huanchaco’s beach and Sausacocha lake (Huamachuco), La Libertad. As biosorption was carried out in four column systems, with 2 g of algae pellets each, circulating As solutions of 0.25 and 1.25 ppm, respectively, at 300 mL/min cm2. As concentration was determined at 3 and 6 h of treatment by flame atomic absorption spectrophotometry. Data were analyzed using Student’s t-test with 95% confidence. At 6 h, Chondracanthus chamissoi presented an As biosorption of 95.76% in a 0.25 ppm mg/L solution and 85.33% in a 1.25 mg/L solution. Cladophora sp., at 6 h, presented an As biosorption of 95.76% in a 0.25 mg/L solution and 42.03% in a 1.25 mg/L solution. It was concluded that Chondracanthus chamissoi achieves higher percentages of biosorption than Cladophora s... [more]

Polysaccharides are biological macromolecules that are difficult to absorb into intestinal epithelial cells for exerting activities, whereas the interaction between polysaccharides and gut microbiota might be an alternative method. This study aimed to explore the in vitro digestion of hawthorn polysaccharides (HPS) and their interaction with the gut microbiota. Results showed that the content of reducing sugars increased slightly during gastric digestion. However, no free monosaccharide was detected during the whole simulated digestion process, indicating that HPS was indigestible. The total carbohydrate residue decreased during in vitro fermentation. This result was due to the utilization by the gut microbiota. Meanwhile, short-chain fatty acids were produced due to the utilization of HPS. Notably, HPS could significantly modulate the composition of human gut microbiota; in particular, the relative abundances of Megasphaera, Acidaminococcus and Mitsuokella increased, whereas the relat... [more]

is one of the first colonizers of bacterial biofilm in water systems and a member of opportunistic premise plumbing pathogens (OPPPs). The aim of this study was to examine the effect of UV light and sodium hypochlorite on the formation and destruction of mature P. fluorescens biofilm on ceramic tiles. Planktonic bacteria or bacteria in mature biofilm were exposed to UV light (254 nm) for 5, 20 s. and to 0.4 mg/L sodium hypochlorite for 1 min. Mature biofilm was also exposed to increased concentration of sodium hypochlorite of 2 mg/L for 0.5, 1 and 2 h and combined with UV. Prolonged action of sodium hypochlorite and an increase in its concentration in combination with UV gave the best results in the inhibition of biofilm formation after the pre-treatment and destruction of mature biofilm. The effect of hyperchlorination in combination with UV radiation shows better results after a long exposure time, although even after 120 min there was no completely destroyed biofilm. Furthermore, th... [more]

In this study, we analyze the occurrence of the unique structural motif, the 3β-corner, belonging to the Structural Classification of Proteins (SCOP) folds, in proteins of various origins. We further assess the structural and functional role of this motif as well as the clustering of the biological functions of proteins in which it occurs. It has been shown previously that the 3β-corner occurs with different probabilities in all beta proteins, alpha and beta proteins (α + β and α/β), and alpha classes occur most often in the composition of β-proteins. The 3β-corner is often found as a building block in protein structures, such as β-barrels, -sandwiches, and -sheets/-layers.

Raltitrexed is a classical antifolate drug with antimetabolite and anticancer properties. In this research, we provide its detailed antitrypanosomal inhibition against six Trypanosoma species and investigate its potential mode of action. Molecular dynamics (MD) simulations and in silico analyses were used to track the binding strength and stability. Raltitrexed showed broad-spectrum trypanocidal actions against Trypanosoma brucei brucei GUTat3.1, T. b. rhodesiense IL1501, T. b. gambiense IL1922, T. evansi Tansui, T. equiperdum IVM-t1 and T. congolense IL3000. The estimated IC50 was found to be in the range of 5.18−24.13 µg/mL, indicating inhibition of Trypanosoma in the low micromolar range. Although the co-crystallized ligand had robust hydrogen bonding and lipophilic characteristics, its docking score was only −4.6 compared to raltitrexed’s −7.78, indicating strong binding with T. brucei dihydrofolate reductase-thymidylate synthase (TbDHFR-TS). MD simulations support the strong bindi... [more]

Personalized Medicine (PM) is rapidly advancing in everyday medical practice. Technological advances allow researchers to reach patients more than ever with their discoveries. The critically ill patient is probably the most complex of all, and personalized medicine must make serious efforts to fulfill the desire to “treat the individual, not the disease”. The complexity of critically ill pathologies arises from the severe state these patients and from the deranged pathways of their diseases. PM constitutes the integration of basic research into clinical practice; however, to make this possible complex and voluminous data require processing through even more complex mathematical models. The result of processing biodata is a digitized individual, from which fragments of information can be extracted for specific purposes. With this review, we aim to describe the current state of PM technologies and methods and explore its application in critically ill patients, as well as some of the chal... [more]

Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung scarring condition with the histological characteristic of typical interstitial pneumonia. Injury to alveolar epithelial cells is a critical precursor in the pathogenesis of this disease. The prevalence of IPF is growing exponentially, with substantial morbidity and mortality rates increasing the burden on economic healthcare costs. A multidisciplinary approach for diagnosis is used to rule out the alternative causes of interstitial lung disease. Pirfenidone and nintedanib, two innovative antifibrotic medicines introduced in recent years, have provided therapeutic benefits to many IPF patients, and several IPF medications are in the early phases of clinical trials. However, available medications can cause unpleasant symptoms such as nausea and diarrhoea. More efforts have been made to uncover alternative treatments towards a more personalised patient-centred care and hence improve the outcomes in the IPF patients. T... [more]

(Bl) is a Gram-positive and spore-forming bacterium belonging to the Brevibacillus brevis phylogenetic cluster. Globally, insect pathogenic strains of the bacterium have been isolated, characterised, and some activities have been patented. Two isolates, Bl 1821L and Bl 1951, exhibiting pathogenicity against the diamondback moth and mosquitoes, are under development as a biopesticide in New Zealand. However, due to the suspected activity of putative antibacterial proteins (ABPs), the endemic isolates often grow erratically. Various purification methods, including size exclusion chromatography, sucrose density gradient centrifugation, polyethylene glycol precipitation, and ammonium sulphate precipitation employed in this study, enabled the isolation of two putative antibacterial proteins of ~30 and ~48 kD from Bl 1821L and one putative antibacterial protein of ~30 kD from Bl 1951. Purification of the uninduced cultures of Bl 1821L and Bl 1951 also yielded protein bands of ~30 and ~48 kD... [more]

Wild bitter gourd extracts, such as saponins, polysaccharides, and peptides, could be used to adjust blood sugar. The objective of this research was to explore the use of high pressure processing (HPP) for sterilization and acceleration of enzyme hydrolysis in the ultrasonic preparation of peptide extracts from wild bitter gourd. The results showed that the wild bitter gourd powder could be extracted via ultrasonic processing with water at 70 °C for only 20 min with a solid to liquid ratio of 1:20 to obtain the total protein content of 1.514 mg/g. The two sterilization methods for wild bitter gourd extract treated with papaya enzyme—for 2 h in the traditional autoclave at 121 °C for 15 min, or under HPP 300 MPa for 5 min—showed no significant effect on protein content, and both sterilization methods were effective. However, the extract sterilized with HPP had a significantly higher ability to scavenge DPPH free radicals. In addition, HPP (300 MPa for 5 min), combined with papaya enzyme... [more]

It is crucial to address the dietary problems of hypertensive patients. The effect and mechanism of different contents of soybean protein on cooking quality and angiotensin-converting enzyme (ACE) inhibitory action in the extruded rice were firstly investigated. The results showed that the extruded rice with soybean protein possessed the higher taste value (90.32 ± 2.31), hardness (2.65 ± 0.01 g), and good pasting quality (p ≤ 0.05). Meanwhile, the soybean protein notably retarded the starch digestibility; the sample with 6% soybean protein showed the fewest rapidly digestible starch (RDS) content (78.82 ± 0.01 mg g−1) and the most slowly digestible starch (SDS) content (8.97 ± 0.45 mg g−1). Importantly, the ACE inhibition rate improved from 17.09 ± 0.01% to 74.02 ± 0.65% in the 6% soybean protein sample because of the production of peptides. The peptide composition of samples were compared, which showed that the effective ACE-inhibitory peptides usually contain 2~20 amino acids, and P... [more]

The thriving agro-industry sector accounts for an essential part of the global gross domestic product, as the need for food and feed production is rising. However, the industrial processing of agricultural products requires the use of water at all stages, which consequently leads to the production of vast amounts of effluents with diverse characteristics, which contain a significantly elevated organic content. This fact reinforces the need for action to control and minimize the environmental impact of the produced wastewater, and activated sludge systems constitute a highly reliable solution for its treatment. The current review offers novel insights on the efficiency of aerobic biosystems in the treatment of agro-industrial wastewaters and their ecology, with an additional focus on the biotechnological potential of the activated sludge of such wastewater treatment plants.

To investigate the molecular mechanism and simulated validation of Zhi Bai Di Huang Pill (ZBDHP) for the treatment of systemic lupus erythematosus (SLE) using network pharmacology and molecular docking techniques. Methods: The active ingredients of ZBDHP were obtained through the TCMSP database and the Canonical SMILES of the active ingredients were queried through Pubchem. The targets of the active ingredients were predicted in the SwissTarget database based on the SMILES. The SLE-related disease targets were obtained through the GeneCards, OMIM and DisGenets databases, and the intersection targets of ZBDHP and SLE were obtained using the Venny 2.1.0 online platform. Intersection targets build a visual protein interaction network (PPI) through the STRING database, and the core targets were identified by network topology analysis. GO analysis and KEGG pathway enrichment analysis of the intersecting targets were performed using the DAVID database. Finally, the molecular docking of the f... [more]