Hydrogen is seen as a future energy carrier since its chemical compounds make up a large part of the Earth’s surface. This study sought to analyze the impact related to the inclusion of hydrogen and oxygen gases produced on demand by an alkaline electrolyzer to the engine added directly through the fuel intake line. For this purpose, performance parameters were monitored, such as liquid fuel consumption and greenhouse gas emissions, and correlated to any effect observed on the engine’s power output and combustion behavior. A 58 kVA nominal power motor-generator was used, coupled with a resistive load bank (20 kW), where two fuel configurations were tested (diesel injection only and a mixture of diesel, hydrogen and oxygen) and compared. A total of 42 tests were performed considering both the admission gases into the fuel intake line and also diesel supply only for baseline. A substantial decrease in fuel consumption was observed (7.59%) when the blend configuration was used despite a decrease in the engine’s work (1.07%). It was also possible to see a common pattern between NO and NO2 emissions for both fuel configurations, while the behavior of the CO2 and CO emissions indicated a higher complete diesel burning fraction when using the gases on demand. Therefore, we can verify that the use of hydrogen and oxygen gases produced on demand in the fuel intake line is a promising alternative to provide a decrease in liquid fuel consumption and an overall improvement in engine combustion.