The use of simulation and modelling has been proposed for determining the excitations to be applied in the procedures of laboratory testing of a car’s structural components, without the need to test the complete vehicle. The paper presents the general concept as well as an example of the procedure. It covers determining the spectrum and time-domain realization of a load on a selected node of the vehicle structure under durability tests. The author used both the mathematical and physical model of the tractor-semitrailer unit, where the input was considered as a random process resulting from the road profile. He calculated the transmittance modules and the power spectral densities of the vertical force on the joint between the tractor’s fifth wheel and the kingpin of the semitrailer and the extreme values of the dynamic components of this force. The inverse discrete Fourier transform makes it possible to generate the realization of the said force. It can be used in durability studies. The limitation of the work to the testing of a specific structural node reduces the scope and, in consequence, the cost of the process. The method presented may also be used for various types of vehicle models, including hybrid and electric vehicles.