The influence of impellers system, baffles system and type of liquid on gas hold-up in a vessel has been presented in this paper. The analysis of gas hold-up was conducted on the basis of the data obtained in the vessel. The vessel used in the study was of inner diameter D = 0.288 m, and it was filled with liquid up to a height of H = 0.576 m. The vessel used in the study was equipped in four planar standard baffles or 24 vertical tubular baffles located on the circuit. A high-speed impellers system, consisting of two impellers located on the shaft, was used to agitate the liquid. The six gas−liquid systems were tested. The gas used in the study was air. The liquids were distilled water, aqueous solutions of NaCl (concentration c = 0.4 kmol/m3 or 0.8 kmol/m3), aqueous solution of sucrose (concentration c = 2.5% mass., 5% mass.), 5% mass. aqueous solution of sucrose and yeast suspension concentration ys = 1% mass. The obtained set of over 1600 experimental points allowed to derive the equations describing the effect of gas flow number Kg, Weber number We and parameter Y (for air−water and air−aqueous solution of NaCl) and Kg, We, c and ys (for air−water, air−aqueous solution of sucrose and air−yeast suspension−aqueous solution of sucrose) on gas hold-up. These equations do not have equivalents in the literature.