The ventilation system plays a crucial role in every building. Proper design and optimization of its operation increase the comfort of users due to efficient air exchange and at the same time control its velocity in the rooms. The aim of this paper is the analysis of the effect of plenum box entry on the velocity profile concerning the diffuser face panel. This issue may sometimes be ignored at the design stage but can significantly affect the airflow from the diffuser and consequently increase the risk of draft. The results of the PIV experimental measurements and numerical simulations concerning various entries of the plenum box (top and side) were investigated in this study. The measurements were used to develop the mathematical and numerical models, which were then used to assess the effect of localization of the spigot of the plenum box on its operation. The numerical analysis was carried out on plenum boxes with the air diffuser with a face panel composed of square grid perforations. Analyses show that the entries significantly affect both the way of air distribution inside the plenum box and the profile of the airflow and its velocity under the simulated air diffuser.