Abstract
The effects of hydrolysis methods (hydrothermal, acid, alkali, hydrothermal-enzyme, acid-enzyme, and alkali-enzyme) on hydrolysate characteristics and photo fermentative hydrogen production (PFHP) of corn straw (CS) and sorghum straw (SS) were investigated. The optimum production of reducing the sugar of straw in different solvent environments was studied by one-step hydrolysis and co-enzymatic hydrolysis pretreatment through a 3,5-dinitrosalicylic acid method. The hydrogen production process by photolytic fermentation of hydrolysates of Rhodobacter sphaeroides HY01 was further analyzed through a gas chromatograph, including the differences in accumulated PFHP yield, chemical oxygen consumption (COD), and volatile fatty acid (VFA) composition. The results showed that the highest reducing sugar yield was obtained by the acid method among one-step hydrolysis. In contrast, acid-enzyme hydrolysis can further increase the reducing sugar yield, which reached 0.42 g·g−1-straw of both straws. Both CS and SS had the highest hydrogen yield from acid-enzyme hydrolysate, 122.72 ± 3.34 mL·g−1-total solid of straw (TS) and 170.04 ± 4.12 mL·g−1-TS, respectively, compared with their acid hydrolysates with 40.46% and 10.53% higher hydrogen yields, respectively. The use of enzymatic hydrolysis showed a significantly higher hydrogen yield for CS compared to SS, indicating that acid hydrolysis was more suitable for SS and acid-enzyme hydrolysis was more suitable for CS.