Abstract
Microalgal biomass is a promising alternative and renewable substrate for bioenergy production. The main problem for its commercial application is to obtain and keep a high level of production by providing microalgae with appropriate conditions for growth. The aim of this study was to determine optimal culture conditions such as temperature, photoperiod, and pH. The amount of biomass by gravimetry, optical density by spectrophotometry, and productivity were analyzed. Suitable values of cultivation parameters allowed for the increased growth and biomass productivity of Arthrospira platensis (4.24 g·L−1), Chlamydomonas reinchardtii (1.19 g·L−1), Chlorella vulgaris (2.37 g·L−1), and Dunaliella salina (4.50 g·L−1) and optical density for Ch. reinchardtii and C. vulgaris. These species had maximum biomass productivity of 0.72, 0.12, 0.36, and 0.77 g·L−1·d−1, respectively. Productivity was determined by cultivation temperature and for Ch. reinchardtii also by pH.