This work provides a case study on the indoor environment and ventilation rate of naturally ventilated research student rooms in Chinese universities. In the measured room, air temperature, relative humidity and carbon dioxide (CO2) concentration were monitored during the heating period for 4 weeks. The number of indoor occupants, occupied time of the room and window/door-opening cases were simultaneously recorded. Results showed the research student room was occupied for an average of 12.0 h each day. Due to a large indoor and outdoor temperature difference during the heating season, and occupants’ adaption to indoor environment, indoor occupants seldom open windows/doors for ventilation. Air exchange of the room only by air infiltration cannot meet the ventilation requirement. As a result, an average of 77.6% of measured CO2 data each day exceeded 1000 ppm during occupied time. In fact, according to CO2 data, it was observed that window/door opening could effectively decrease indoor CO2 concentration. Therefore, intermittent window/door opening or CO2-based demand-controlled ventilation facilities were suggested for improving indoor air quality of such rooms. Additionally, special attention should be paid to other possible outdoor pollution.