Bioconversion of the Brown Tunisian Seaweed Halopteris scoparia: Application to Energy

Farah Hadjkacem; Guillaume Pierre; Gwendoline Christophe; Jihen Elleuch; Imen Fendri; Zakaria Boual; Mohamed Didi Ould El Hadj; Zainab El Alaoui-Talibi; Cherkaoui El Modafar; Pascal Dubessay; Cédric Delattre; Philippe Michaud; Slim Abdelkafi

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2023.12491

Published Article

LAPSE:2023.12491

Bioconversion of the Brown Tunisian Seaweed Halopteris scoparia: Application to Energy

Farah Hadjkacem, Guillaume Pierre, Gwendoline Christophe, Jihen Elleuch, Imen Fendri, Zakaria Boual, Mohamed Didi Ould El Hadj, Zainab El Alaoui-Talibi, Cherkaoui El Modafar, Pascal Dubessay, Cédric Delattre, Philippe Michaud, Slim Abdelkafi

February 28, 2023

The brown Tunisian seaweed Halopteris scoparia was used as a feedstock for producing renewable bioethanol, biogas, and biodiesel to demonstrate the proof of concept for the North African energy sector. A quantitative and qualitative quantification of H. scoparia composition using different colorimetric methods was completed to highlight its bioconversion potential. These substrate inputs were subjected to anaerobic fermentation by Saccharomyces cerevisiae to produce bioethanol. The materials were also used to generate bio-hydrogen and volatile fatty acids during dark fermentation by a bacterial consortium and using the oleaginous yeast Yarrowia lipolytica. The lipids were extracted and trans-esterified to Fatty Acid Methyl Esters (FAMEs), and their profiles were then analyzed with gas chromatography (GC). A significant ratio of the bioethanol, e.g., 0.35 g ethanol/g DW substrate, was produced without pretreatment, consistent with the theoretical Gay-Lussac yield. The production of the biohydrogen and lipids were up to 1.3 mL H2/g DW substrate and 0.04 g/g DW substrate, respectively, from the raw biomass. These results were higher than those reported for other well-studied seaweeds such as L. japonica. Overall, this work contributes to the current investigations in Tunisia for producing alternative energies from algae and finding new solutions to the current energy situation and environmental challenges in Maghreb.

Record ID

LAPSE:2023.12491

Keywords

bio-hydrogen, by-product, Ethanol, Fermentation, Halopteris scoparia, lipid

Subject

Materials

Suggested Citation

Hadjkacem F, Pierre G, Christophe G, Elleuch J, Fendri I, Boual Z, Ould El Hadj MD, El Alaoui-Talibi Z, El Modafar C, Dubessay P, Delattre C, Michaud P, Abdelkafi S. Bioconversion of the Brown Tunisian Seaweed Halopteris scoparia: Application to Energy. (2023). LAPSE:2023.12491

Author Affiliations

Hadjkacem F: Laboratoire de Génie Enzymatique et Microbiologique, Equipe de Biotechnologie des Algues, Ecole Nationale d’Ingénieur de Sfax, Sfax 3038, Tunisia; Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, F
Pierre G: Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France [ORCID]
Christophe G: Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France
Elleuch J: Laboratoire de Génie Enzymatique et Microbiologique, Equipe de Biotechnologie des Algues, Ecole Nationale d’Ingénieur de Sfax, Sfax 3038, Tunisia
Fendri I: Laboratory of Plant Biotechnologies Applied to the Improvement of Plants, Faculty of Sciences, University of Sfax, Sfax 3038, Tunisia [ORCID]
Boual Z: Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria [ORCID]
Ould El Hadj MD: Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria
El Alaoui-Talibi Z: Centre d’Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech-URL-CNRST-05), Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakech 40000, Morocco
El Modafar C: Centre d’Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech-URL-CNRST-05), Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakech 40000, Morocco
Dubessay P: Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France
Delattre C: Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France; Institut Universitaire de France (IUF), 1 Rue Descartes, F-75005 Paris, France [ORCID]
Michaud P: Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France [ORCID]
Abdelkafi S: Laboratoire de Génie Enzymatique et Microbiologique, Equipe de Biotechnologie des Algues, Ecole Nationale d’Ingénieur de Sfax, Sfax 3038, Tunisia [ORCID]

Journal Name

Energies

Volume

15

Issue

12

First Page

4342

Year

2022

Publication Date

2022-06-14