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LAPSE:2023.2815

Spray-Dried Powder Containing Chitinase and β-1,3-Glucanase with Insecticidal Activity against Ceratitis capitata (Diptera: Tephritidae)

Bruno C. Aita, Silvana Schmaltz, Alex Fochi, Vinícius F. Bolson, Thiarles Brun, Lucas de Arruda Cavallin, Gabriel Camatti, Dori E. Nava, Jerson V. C. Guedes, Raquel C. Kuhn, Giovani L. Zabot, Marcus Vinícius Tres, Marcio A. Mazutti

February 21, 2023

Abstract
This study focused on obtaining a spray-dried powder containing chitinase and β-1,3-glucanase as active ingredients for the control of agricultural pests. Different carriers were tested in the spray drying of these enzymes. The effectiveness of the application of the enzymes was evaluated against Ceratitis capitata (Diptera: Tephritidae). The combination of maltodextrin (2.5% w/v), gum Arabic (2.5% w/v), and soluble starch (5.0% w/v) as carriers showed the best result of residual activity of β-1,3-glucanase (88.36%) and chitinase (69.82%), with a powder recovery of 45.49%. The optimum conditions for the operational parameters of the spray drying process were: inlet air temperature of 120 °C, drying airflow rate of 1.1 m3/min, feed flow rate of 5.8 mL/min, and nozzle air pressure of 0.4 MPa. The powder produced showed 65.6% efficiency for the control of the fly. These results demonstrated the possibility of using the spray drying process to obtain an enzymatic potential product for biological pest control.

Record ID

LAPSE:2023.2815

Keywords

biocontrol, cell wall degrading enzymes, microparticles, spray drying

Subject

Process Control

Suggested Citation

Aita BC, Schmaltz S, Fochi A, Bolson VF, Brun T, de Arruda Cavallin L, Camatti G, Nava DE, Guedes JVC, Kuhn RC, Zabot GL, Tres MV, Mazutti MA. Spray-Dried Powder Containing Chitinase and β-1,3-Glucanase with Insecticidal Activity against Ceratitis capitata (Diptera: Tephritidae). (2023). LAPSE:2023.2815

Author Affiliations

Aita BC: Department of Chemical Engineering, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil
Schmaltz S: Department of Chemical Engineering, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil
Fochi A: Department of Chemical Engineering, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil
Bolson VF: Department of Chemical Engineering, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil
Brun T: Department of Agricultural Engineering, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil
de Arruda Cavallin L: Department of Plant Protection, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil
Camatti G: Department of Plant Protection, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil
Nava DE: Embrapa Clima Temperado, Entomology Laboratory, Pelotas 96010-971, RS, Brazil
Guedes JVC: Department of Plant Protection, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil [ORCID]
Kuhn RC: Department of Chemical Engineering, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil [ORCID]
Zabot GL: Laboratory of Agroindustrial Processes Engineering (LAPE), Federal University of Santa Maria (UFSM), Cachoeira do Sul 96508-010, RS, Brazil
Tres MV: Laboratory of Agroindustrial Processes Engineering (LAPE), Federal University of Santa Maria (UFSM), Cachoeira do Sul 96508-010, RS, Brazil [ORCID]
Mazutti MA: Department of Chemical Engineering, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil

Journal Name

Processes

Volume

Issue

First Page

587

Year

2022

Publication Date

2022-03-17

DOI:

https://doi.org/10.3390/pr10030587