LAPSE:2023.0113
Published Article
LAPSE:2023.0113
In Vitro Antithrombotic, Antitumor and Antiangiogenic Activities of Green Tea Polyphenols and Its Main Constituent Epigallocatechin-3-gallate
February 17, 2023
Abstract
The balance between embolic risk and bleeding represents a clinical challenge in cancer patient treatment, encouraging studies on adjuvant oncologic treatments. Thereby, this study evaluated the in vitro effect of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on hemostasis modulation and the antineoplastic effect on melanoma cells (B16-F10) by applying platelet aggregation, angiogenesis and viability cell assays. The results displayed a significant platelet antiaggregant effect, corresponding to 50 and 80% for the extract and EGCG, respectively, compared to the negative control. Furthermore, both GTE and EGCG exhibited antitumor effects by reducing melanoma cell growth by 25 and 50%, respectively, verified by cellular apoptosis. Regarding angiogenesis, these substances inhibited blood vessel formation, reaching about 25% and 99% for GTE and EGCG at 100 μg/mL, respectively. Moreover, TNF-α cell stimulation evidenced VEGF and IL-8 secretion inhibition at 55 and 20% with GTE, while EGCG promoted an inhibition around 78% for both VEGF and IL-8. The results indicate the promising performance of GTE and EGCG as an option for treating cancer and its side effects. Nonetheless, further studies are required to elucidate their action mechanism on clotting, cell death and angiogenesis.
The balance between embolic risk and bleeding represents a clinical challenge in cancer patient treatment, encouraging studies on adjuvant oncologic treatments. Thereby, this study evaluated the in vitro effect of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on hemostasis modulation and the antineoplastic effect on melanoma cells (B16-F10) by applying platelet aggregation, angiogenesis and viability cell assays. The results displayed a significant platelet antiaggregant effect, corresponding to 50 and 80% for the extract and EGCG, respectively, compared to the negative control. Furthermore, both GTE and EGCG exhibited antitumor effects by reducing melanoma cell growth by 25 and 50%, respectively, verified by cellular apoptosis. Regarding angiogenesis, these substances inhibited blood vessel formation, reaching about 25% and 99% for GTE and EGCG at 100 μg/mL, respectively. Moreover, TNF-α cell stimulation evidenced VEGF and IL-8 secretion inhibition at 55 and 20% with GTE, while EGCG promoted an inhibition around 78% for both VEGF and IL-8. The results indicate the promising performance of GTE and EGCG as an option for treating cancer and its side effects. Nonetheless, further studies are required to elucidate their action mechanism on clotting, cell death and angiogenesis.
Record ID
Keywords
anticoagulant, apoptosis, cancer, green tea, melanoma, toxicity
Subject
Suggested Citation
Luz JRDD, López JA, Ferreira MP, de Sousa RM, Silva SVE, Almeida MDG, Araujo-Silva G. In Vitro Antithrombotic, Antitumor and Antiangiogenic Activities of Green Tea Polyphenols and Its Main Constituent Epigallocatechin-3-gallate. (2023). LAPSE:2023.0113
Author Affiliations
Luz JRDD: Organic Chemistry and Biochemistry Laboratory, State University of Amapá (UEAP), Av. Presidente Vargas, s/n-Centro, Macapá 68900-070, AP, Brazil; Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Scien [ORCID]
López JA: Organic Chemistry and Biochemistry Laboratory, State University of Amapá (UEAP), Av. Presidente Vargas, s/n-Centro, Macapá 68900-070, AP, Brazil; Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Scien
Ferreira MP: Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Sciences Center, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Farias, s/n-Petrópolis, Natal 59012-570, RN, Brazil
de Sousa RM: Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Sciences Center, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Farias, s/n-Petrópolis, Natal 59012-570, RN, Brazil; Postgraduat
Silva SVE: Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Sciences Center, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Farias, s/n-Petrópolis, Natal 59012-570, RN, Brazil; Postgraduat [ORCID]
Almeida MDG: Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Sciences Center, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Farias, s/n-Petrópolis, Natal 59012-570, RN, Brazil; Postgraduat [ORCID]
Araujo-Silva G: Organic Chemistry and Biochemistry Laboratory, State University of Amapá (UEAP), Av. Presidente Vargas, s/n-Centro, Macapá 68900-070, AP, Brazil
López JA: Organic Chemistry and Biochemistry Laboratory, State University of Amapá (UEAP), Av. Presidente Vargas, s/n-Centro, Macapá 68900-070, AP, Brazil; Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Scien
Ferreira MP: Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Sciences Center, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Farias, s/n-Petrópolis, Natal 59012-570, RN, Brazil
de Sousa RM: Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Sciences Center, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Farias, s/n-Petrópolis, Natal 59012-570, RN, Brazil; Postgraduat
Silva SVE: Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Sciences Center, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Farias, s/n-Petrópolis, Natal 59012-570, RN, Brazil; Postgraduat [ORCID]
Almeida MDG: Multidisciplinary Research Laboratory, Department of Clinical and Toxicological Analyses DACT, Health Sciences Center, Federal University of Rio Grande do Norte, R. Gen. Gustavo Cordeiro de Farias, s/n-Petrópolis, Natal 59012-570, RN, Brazil; Postgraduat [ORCID]
Araujo-Silva G: Organic Chemistry and Biochemistry Laboratory, State University of Amapá (UEAP), Av. Presidente Vargas, s/n-Centro, Macapá 68900-070, AP, Brazil
Journal Name
Processes
Volume
11
Issue
1
First Page
76
Year
2022
Publication Date
2022-12-28
ISSN
2227-9717
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Original Submission
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PII: pr11010076, Publication Type: Journal Article
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LAPSE:2023.0113
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https://doi.org/10.3390/pr11010076
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Feb 17, 2023
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