LAPSE:2024.0433
Published Article
LAPSE:2024.0433
Co-Product of Pracaxi Seeds: Quantification of Epicatechin by HPLC-DAD and Microencapsulation of the Extract by Spray Drying
June 5, 2024
Abstract
In the industrial processing of fruits, co-products are generated, which are often not used. The pracaxi co-product, obtained by cold pressing its seeds, contains phenolic compounds with antioxidant activity, which in this work were extracted and microencapsulated by spray drying. The pracaxi extract was characterized by Fourier-transform infrared spectroscopy (FTIR) and high-performance liquid chromatography (HPLC-DAD), and its antioxidant activity was quantified by the ABTS and DPPH assays. Total polyphenol and flavonoid contents in the extract and microparticles were determined by UV-Vis spectrophotometry. Microparticles were then characterized regarding their moisture content, morphology (by scanning electron microscopy), size, polydispersity index and zeta potential. The FTIR spectra revealed functional groups that may be related to phenolic compounds. The extract showed good antioxidant activity according to both selected assays, while the HPLC-DAD analysis evidenced epicatechin as the main compound, whose content was quantified and validated according to the guidelines of recognized national and international agencies. The total polyphenol contents were 20.61 ± 0.20 mg gallic acid equivalent (GAE)/g in the extract and 18.48 ± 0.10 mg GAE/g in the microparticles, while the total flavonoid contents were 28.29 ± 0.70 mg quercetin equivalent (QE)/g and 13.73 ± 0.10 mg QE/g, respectively. Microparticles had a low moisture content, spherical shape, size less than 1 μm and negative zeta potential. Furthermore, they were able to satisfactorily retain phenolic compounds, although in a smaller amount compared to the extract due to small losses resulting from degradation during the drying process. These results, taken as a whole, demonstrate that the pracaxi co-product can be a promising candidate in obtaining products of interest for the cosmetic and food sectors by aiming to exploit its antioxidant activity.
Keywords
Amazonian fruits, antioxidant activity, flavonoids, spray drying, validation
Suggested Citation
da Silva RL, Ferreira LMDMC, Silva-Júnior JOC, Converti A, Ribeiro-Costa RM. Co-Product of Pracaxi Seeds: Quantification of Epicatechin by HPLC-DAD and Microencapsulation of the Extract by Spray Drying. (2024). LAPSE:2024.0433
Author Affiliations
da Silva RL: Laboratory of Pharmaceutical Nanotechnology, College of Pharmacy, Federal University of Pará, Belém 66075-110, PA, Brazil
Ferreira LMDMC: Laboratory of Pharmaceutical Nanotechnology, College of Pharmacy, Federal University of Pará, Belém 66075-110, PA, Brazil [ORCID]
Silva-Júnior JOC: Laboratory of Pharmaceutical and Cosmetic R&D, College of Pharmacy, Federal University of Pará, Belém 66075-110, PA, Brazil [ORCID]
Converti A: Department of Civil, Chemical and Environmental Engineering, University of Genoa, Pole of Chemical Engineering, Via Opera Pia 15, 16145 Genova, Italy [ORCID]
Ribeiro-Costa RM: Laboratory of Pharmaceutical Nanotechnology, College of Pharmacy, Federal University of Pará, Belém 66075-110, PA, Brazil [ORCID]
Journal Name
Processes
Volume
12
Issue
5
First Page
997
Year
2024
Publication Date
2024-05-14
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr12050997, Publication Type: Journal Article
Record Map
Published Article

LAPSE:2024.0433
This Record
External Link

https://doi.org/10.3390/pr12050997
Publisher Version
Download
Files
Jun 5, 2024
Main Article
License
CC BY 4.0
Meta
Record Statistics
Record Views
316
Version History
[v1] (Original Submission)
Jun 5, 2024
 
Verified by curator on
Jun 5, 2024
This Version Number
v1
Citations
Most Recent
This Version
URL Here
https://psecommunity.org/LAPSE:2024.0433
 
Record Owner
Auto Uploader for LAPSE
Links to Related Works
Directly Related to This Work
Publisher Version