The Improved Cytotoxic Capacity of Functionalized Nanodiamonds with Metformin in Breast and Ovarian Cancer Cell Lines

Lucero Evelia Acuña-Aguilar; Alain Salvador Conejo-Dávila; Mario Miki-Yoshida; Olga N. Hernández-de la Cruz; Gricelda Sánchez-Sánchez; César López-Camarillo; Joan Sebastian Salas-Leiva; Erasto Armando Zaragoza-Contreras; Reyna Reyes-Martínez; Erasmo Orrantia-Borunda

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2024.0070

Published Article

LAPSE:2024.0070

The Improved Cytotoxic Capacity of Functionalized Nanodiamonds with Metformin in Breast and Ovarian Cancer Cell Lines

Lucero Evelia Acuña-Aguilar, Alain Salvador Conejo-Dávila, Mario Miki-Yoshida, Olga N. Hernández-de la Cruz, Gricelda Sánchez-Sánchez, César López-Camarillo, Joan Sebastian Salas-Leiva, Erasto Armando Zaragoza-Contreras, Reyna Reyes-Martínez, Erasmo Orrantia-Borunda

January 12, 2024

Nanodiamonds (ND-COOH) are used as drug delivery systems because of their attractive properties, as they allow for optimized transport of therapeutic agents in cellular models. Metformin (MET) is a drug used in diabetes mellitus therapy and exhibits anti-cancer properties. In this study, dispersed nanodiamonds were functionalized with metformin by directly binding them to 1,6-hexanediol (ND-MET), and their effects on the cytotoxicity of breast and ovarian cancer cells were evaluated in vitro. A simple synthesis of ND-MET was performed and characterized using FT-IR, XPS, Boehm titration, RAMAN, XDR, TEM, and dynamic light scattering (DLS). Data showed an increased intensity of the C-N bond band, indicating the presence of metformin in ND-MET. We detected signals at 1427 cm−1 and 1288 cm−1 corresponding to the C-N and C-H bonds, and adsorptions at 1061 cm−1 and 3208 cm−1 corresponding to the N-O and N-H bonds, respectively. The deconvolution of the C1s binding energy was also found at 286.24 eV. The biological effects of ND-MET were tested in both SKOV3 ovarian cancer and Hs-578T and MDA-MB-231 triple-negative breast cancer cell lines. In SKOV3 cells, the IC50 for the ND-MET complex was 35 ± 14 µg/mL, while for Hs-578T and MDA-MB-231 breast cancer cells, the IC50 for ND-MET was 759 ± 44 µg/mL and 454 ± 49 µg/mL, respectively. Our data showed that ND-MET could be used as an intracellular delivery system for metformin in cancer cells. Cell viability assays evidenced a reduced viability of all cell lines in a time and dose-dependent manner, with a significant sensitivity observed in SKOV3 ovarian cancer cells treated with ND-MET.

Record ID

LAPSE:2024.0070

Keywords

biguanide, breast cancer, functionalization, nanodiamonds, ovarian cancer

Subject

Biosystems

Suggested Citation

Acuña-Aguilar LE, Conejo-Dávila AS, Miki-Yoshida M, Hernández-de la Cruz ON, Sánchez-Sánchez G, López-Camarillo C, Salas-Leiva JS, Zaragoza-Contreras EA, Reyes-Martínez R, Orrantia-Borunda E. The Improved Cytotoxic Capacity of Functionalized Nanodiamonds with Metformin in Breast and Ovarian Cancer Cell Lines. (2024). LAPSE:2024.0070

Author Affiliations

Acuña-Aguilar LE: Departamento de Medio Ambiente y Energía, Centro de Investigación en Materiales Avanzados, S.C. Av. Miguel de Cervantes Saavedra 120, Chihuahua 31136, Mexico [ORCID]
Conejo-Dávila AS: Departamento de Medio Ambiente y Energía, Centro de Investigación en Materiales Avanzados, S.C. Av. Miguel de Cervantes Saavedra 120, Chihuahua 31136, Mexico; Centro de Innovación Aplicada en Tecnologías Competitivas, A.C. Calle Omega No. 201, Delta,
Miki-Yoshida M: Departamento de Medio Ambiente y Energía, Centro de Investigación en Materiales Avanzados, S.C. Av. Miguel de Cervantes Saavedra 120, Chihuahua 31136, Mexico
Hernández-de la Cruz ON: Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Ciudad de México 06720, Mexico
Sánchez-Sánchez G: Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Ciudad de México 06720, Mexico
López-Camarillo C: Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Ciudad de México 06720, Mexico [ORCID]
Salas-Leiva JS: Departamento de Medio Ambiente y Energía, Centro de Investigación en Materiales Avanzados, S.C. Av. Miguel de Cervantes Saavedra 120, Chihuahua 31136, Mexico; CONAHCyT-Departamento de Medio Ambiente y Energía, Centro de Investigación en Materiales Ava [ORCID]
Zaragoza-Contreras EA: Departamento de Medio Ambiente y Energía, Centro de Investigación en Materiales Avanzados, S.C. Av. Miguel de Cervantes Saavedra 120, Chihuahua 31136, Mexico [ORCID]
Reyes-Martínez R: Departamento de Química, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Ciudad Universitaria, Campus 2, Chihuahua 31110, Mexico
Orrantia-Borunda E: Departamento de Medio Ambiente y Energía, Centro de Investigación en Materiales Avanzados, S.C. Av. Miguel de Cervantes Saavedra 120, Chihuahua 31136, Mexico

Journal Name

Processes

Volume

11

Issue

9

First Page

2616

Year

2023

Publication Date

2023-09-02