LAPSE:2023.9862
Published Article
LAPSE:2023.9862
Assessment of Cytochrome c and Chlorophyll a as Natural Redox Mediators for Enzymatic Biofuel Cells Powered by Glucose
February 27, 2023
Abstract
The development of new high-power biofuel cells has been limited in the past by slow or indirect charge transfer. In this study, enzymatic biofuel cell (EBFC) systems were explored with different materials used to evaluate their applicability as redox mediators. Redox mediators of different natures have been selected for this research. Cytochrome c, Chlorophyll a, and supernatant of ultrasonically disrupted algae Chlorella vulgaris cells were examined as potential redox mediators. The effect of heparin on the EBFC was also evaluated under the same analytical conditions. The measurements of open circuit potential (OCP) and the evaluation of the current response in two modes of measurement were performed (i) during potential cycling in cyclic voltammetry measurements or (ii) at the constant potential value in chronoamperometry, and were applied for the evaluation of EBFC. Cytochrome c, Chlorophyll a, and the supernatant of ultrasonically disrupted algae Chlorella vulgaris cells-based redox mediators were efficient in the glucose oxidase (GOx) based EBFC. Electron transfer from GOx to the electrode was facilitated through the redox mediators adsorbed on the electrode. Electrodes modified with Chlorophyll a- and Cytochrome c-based redox mediators were suitable for the development of glucose biosensors. This was demonstrated by increasing the glucose concentration within 0 mM−100 mM in the system, the current density increased, and the system reached equilibrium rather faster regarding the electrochemical reaction. The power density is an important feature in revealing the action of biofuel cells. The highest power values were generated by the systems based on the application of redox-mediated Chlorophyll a and the supernatant of ultrasonically disrupted Chlorella vulgaris cells. The surface power density was about 2.5−4.0 µW/cm2. Control of a study was performed with a polished graphite electrode and the maximum surface power density was 0.02471 µW/cm2.
The development of new high-power biofuel cells has been limited in the past by slow or indirect charge transfer. In this study, enzymatic biofuel cell (EBFC) systems were explored with different materials used to evaluate their applicability as redox mediators. Redox mediators of different natures have been selected for this research. Cytochrome c, Chlorophyll a, and supernatant of ultrasonically disrupted algae Chlorella vulgaris cells were examined as potential redox mediators. The effect of heparin on the EBFC was also evaluated under the same analytical conditions. The measurements of open circuit potential (OCP) and the evaluation of the current response in two modes of measurement were performed (i) during potential cycling in cyclic voltammetry measurements or (ii) at the constant potential value in chronoamperometry, and were applied for the evaluation of EBFC. Cytochrome c, Chlorophyll a, and the supernatant of ultrasonically disrupted algae Chlorella vulgaris cells-based redox mediators were efficient in the glucose oxidase (GOx) based EBFC. Electron transfer from GOx to the electrode was facilitated through the redox mediators adsorbed on the electrode. Electrodes modified with Chlorophyll a- and Cytochrome c-based redox mediators were suitable for the development of glucose biosensors. This was demonstrated by increasing the glucose concentration within 0 mM−100 mM in the system, the current density increased, and the system reached equilibrium rather faster regarding the electrochemical reaction. The power density is an important feature in revealing the action of biofuel cells. The highest power values were generated by the systems based on the application of redox-mediated Chlorophyll a and the supernatant of ultrasonically disrupted Chlorella vulgaris cells. The surface power density was about 2.5−4.0 µW/cm2. Control of a study was performed with a polished graphite electrode and the maximum surface power density was 0.02471 µW/cm2.
Record ID
Keywords
Chlorophyll a, cytochrome c, glucose, heparin, microbial-based biofuel cell, redox mediators, the supernatant of ultrasonically disrupted Chlorella vulgaris cells
Subject
Suggested Citation
Samukaite Bubniene U, Zukauskas S, Ratautaite V, Vilkiene M, Mockeviciene I, Liustrovaite V, Drobysh M, Lisauskas A, Ramanavicius S, Ramanavicius A. Assessment of Cytochrome c and Chlorophyll a as Natural Redox Mediators for Enzymatic Biofuel Cells Powered by Glucose. (2023). LAPSE:2023.9862
Author Affiliations
Samukaite Bubniene U: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology (FTMC), Sauletekio av. 3, LT-10257 Vilnius, Lithuania; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilni
Zukauskas S: Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania
Ratautaite V: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology (FTMC), Sauletekio av. 3, LT-10257 Vilnius, Lithuania; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilni [ORCID]
Vilkiene M: Institute of Agriculture, Lithuanian Research Center for Agriculture and Forestry (LAMMC), Vezaiciai Branch, Gargzdu Str. 29, Klaipeda district, LT-96216 Vezaiciai, Lithuania
Mockeviciene I: Institute of Agriculture, Lithuanian Research Center for Agriculture and Forestry (LAMMC), Vezaiciai Branch, Gargzdu Str. 29, Klaipeda district, LT-96216 Vezaiciai, Lithuania
Liustrovaite V: Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania
Drobysh M: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology (FTMC), Sauletekio av. 3, LT-10257 Vilnius, Lithuania; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilni [ORCID]
Lisauskas A: Laboratory of Combustion Processes, Lithuanian Energy Institute, Breslaujos Str. 3, LT-44403 Kaunas, Lithuania [ORCID]
Ramanavicius S: Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania; Department of Electrochemical Material Science, State Research Institute Center for Physic [ORCID]
Ramanavicius A: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology (FTMC), Sauletekio av. 3, LT-10257 Vilnius, Lithuania; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilni [ORCID]
Zukauskas S: Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania
Ratautaite V: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology (FTMC), Sauletekio av. 3, LT-10257 Vilnius, Lithuania; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilni [ORCID]
Vilkiene M: Institute of Agriculture, Lithuanian Research Center for Agriculture and Forestry (LAMMC), Vezaiciai Branch, Gargzdu Str. 29, Klaipeda district, LT-96216 Vezaiciai, Lithuania
Mockeviciene I: Institute of Agriculture, Lithuanian Research Center for Agriculture and Forestry (LAMMC), Vezaiciai Branch, Gargzdu Str. 29, Klaipeda district, LT-96216 Vezaiciai, Lithuania
Liustrovaite V: Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania
Drobysh M: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology (FTMC), Sauletekio av. 3, LT-10257 Vilnius, Lithuania; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilni [ORCID]
Lisauskas A: Laboratory of Combustion Processes, Lithuanian Energy Institute, Breslaujos Str. 3, LT-44403 Kaunas, Lithuania [ORCID]
Ramanavicius S: Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania; Department of Electrochemical Material Science, State Research Institute Center for Physic [ORCID]
Ramanavicius A: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology (FTMC), Sauletekio av. 3, LT-10257 Vilnius, Lithuania; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilni [ORCID]
Journal Name
Energies
Volume
15
Issue
18
First Page
6838
Year
2022
Publication Date
2022-09-19
ISSN
1996-1073
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PII: en15186838, Publication Type: Journal Article
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LAPSE:2023.9862
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https://doi.org/10.3390/en15186838
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