Due to the depletion of fossil fuels and the destruction wrought by global warming caused by the combustion of fossil fuels, the search for renewable energy sources has become a major global concern. This study aimed to assess the bio-oil production from teff husk via slow pyrolysis process. The pyrolysis of teff husk took place in a batch reactor at a temperature between 400 °C and 500 °C with a 120 min retention time. At 450 °C, the pyrolysis process produced 32.96 wt.% of optimum bio-oil yield and had a HHV of 25.32 MJ/kg. TGA, FTIR, and SEM-EDX were used to analyze the produced bio-oil to investigate its thermal decomposition, functional groups, and surface morphology with its elemental composition, respectively. Alcohols, aromatic, phenols, alkanes, esters, and ethers were the primary compounds of the bio-oil produced by the slow pyrolysis of teff husk. The HHV of the biochar ranged from 21.22 to 22.85 MJ/kg. As a result, teff husk can be used to make biofuel; however, further bio-oil upgrading is needed for the produced teff husk bio-oil to be used effectively and commercially. Overall, the slow pyrolysis of teff husk offers a chance to produce biofuels with enhanced value that can be used for additional purposes.