Abstract
Pathogenic microbial contamination is significantly influenced by the crop surface properties and surfactant use, which are crucial factors for the postharvest washing process. However, there is little information on the interaction between surfactant and food pathogens on food crops. Thus, this study (1) investigated whether the attachment of Salmonella increases as pesticides denature epicuticular wax crystals and (2) tested if the antibacterial effect of ozonated water can be improved on waxy produce surfaces by adding surfactant to ozonated water. As a result, significantly lower levels of Salmonella Typhimurium attached to waxy leaf surfaces than they did to glossy and pesticide-treated waxy leaf surfaces (3.28 as opposed to 4.10 and 4.32 Log colony forming units (CFU)/cm2, respectively), suggesting that the pesticide containing a surfactant application increased the attachment of S. Typhiumurium on waxy leaf surfaces. There was no significant washing effect on waxy leaf surfaces washed with ozonated water. On the other hand, S. Typhimurium were not detected on waxy leaf surfaces after washing with surfactant-added ozonated water.