Formation of Giant Lipid Vesicles in the Presence of Nonelectrolytes—Glucose, Sucrose, Sorbitol and Ethanol

Qiong Wang; Ning Hu; Jincan Lei; Qiurong Qing; Jing Huang; Ke Tao; Shixian Zhao; Ke Sun; Jun Yang

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2023.5673

Published Article

LAPSE:2023.5673

Formation of Giant Lipid Vesicles in the Presence of Nonelectrolytes—Glucose, Sucrose, Sorbitol and Ethanol

Qiong Wang, Ning Hu, Jincan Lei, Qiurong Qing, Jing Huang, Ke Tao, Shixian Zhao, Ke Sun, Jun Yang

February 23, 2023

Lipid vesicles, especially giant lipid vesicles (GLVs), are usually adopted as cell membrane models and their preparation has been widely studied. However, the effects of some nonelectrolytes on GLV formation have not been specifically studied so far. In this paper, the effects of the nonelectrolytes, including sucrose, glucose, sorbitol and ethanol, and their coexistence with sodium chloride, on the lipid hydration and GLV formation were investigated. With the hydration method, it was found that the sucrose, glucose and sorbitol showed almost the same effect. Their presence in the medium enhanced the hydrodynamic force on the lipid membranes, promoting the GLV formation. GLV formation was also promoted by the presence of ethanol with ethanol volume fraction in the range of 0 to 20 percent, but higher ethanol content resulted in failure of GLV formation. However, the participation of sodium chloride in sugar solution and ethanol solution stabilized the lipid membranes, suppressing the GLV formation. In addition, the ethanol and the sodium chloride showed the completely opposite effects on lipid hydration. These results could provide some suggestions for the efficient preparation of GLVs.

Record ID

LAPSE:2023.5673

Keywords

Ethanol, giant lipid vesicle, glucose, sorbitol, sucrose

Subject

Energy Systems

Suggested Citation

Wang Q, Hu N, Lei J, Qing Q, Huang J, Tao K, Zhao S, Sun K, Yang J. Formation of Giant Lipid Vesicles in the Presence of Nonelectrolytes—Glucose, Sucrose, Sorbitol and Ethanol. (2023). LAPSE:2023.5673

Author Affiliations

Wang Q: Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China
Hu N: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China [ORCID]
Lei J: Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China
Qing Q: Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China
Huang J: Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China
Tao K: Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China
Zhao S: Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China
Sun K: Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China
Yang J: Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China [ORCID]

Journal Name

Processes

Volume

9

Issue

6

First Page

945

Year

2021

Publication Date

2021-05-27