Due to their outstanding properties, carbon-based structures have received much attention from the scientific community. Their applications are diverse and include use in coatings on self-lubricating systems for anti-wear situations, thin films deposited on prosthetic elements, catalysis structures, or water remediation devices. From these applications, the ones that require the most careful testing and improvement are biomedical applications. The biocompatibility and antibacterial issues of medical devices remain a concern, as several prostheses still fail after several years of implantation and biofilm formation remains a real risk to the success of a device. Sputtered deposition prevents the introduction of hazardous chemical elements during the preparation of coatings, and this technique is environmentally friendly. In addition, the mechanical properties of C-based coatings are remarkable. In this paper, the latest advances in sputtering methods and biocompatibility and antibacterial action for diamond-based carbon (DLC)-based coatings are reviewed and the greater outlook is then discussed.