LAPSE:2023.4180v1
Published Article
LAPSE:2023.4180v1
Toxicological Profile of Biological Environment of Two Elastodontic Devices
February 22, 2023
Abstract
Malocclusion and teething problems are common health problems globally, affecting people of all ages, especially children and adolescents. In addition to the pathophysiological complications associated with orthodontic problems, they also affect the well-being of the individual. Orthodontic appliances are frequently used, even from an early age, and their activity in different biological environments is very varied and incompletely described. Due to these considerations, the purpose of the study was to evaluate the toxicological profile of the biological environment (saliva at three pH values: 3, 7, and 10) of two elastodontic orthodontic appliances: Myobrace (MB) and LM TrainerTM 2 (LMD). In vitro techniques applied were conducted on human keratinocytes to evaluate cell viability (Alamar blue assay) and gene expression real-time reverse transcription−polymerase chain reaction (RT-PCR technique). In addition, it was assessed the irritating effect on the vascular plexus using as a biological model the chorioallantoic membrane of the hen’s egg by applying the hen’s egg-chorioallantoic membrane (HET-CAM) method. The obtained results showed a decrease in cell viability up to 82% in the case of LMD at pH = 3, a slight increase in mRNA expression for the anti-apoptotic marker (Bcl-2 and Bcl-xL), and a decrease in mRNA expression for the pro-apoptotic marker (Bad), and any type of toxic change at the capillary level (irritation score being below 0.9). Based on the data obtained, it can be stated that MB and LMD biological environments, at different pH values, present a safe toxicological profile.
Malocclusion and teething problems are common health problems globally, affecting people of all ages, especially children and adolescents. In addition to the pathophysiological complications associated with orthodontic problems, they also affect the well-being of the individual. Orthodontic appliances are frequently used, even from an early age, and their activity in different biological environments is very varied and incompletely described. Due to these considerations, the purpose of the study was to evaluate the toxicological profile of the biological environment (saliva at three pH values: 3, 7, and 10) of two elastodontic orthodontic appliances: Myobrace (MB) and LM TrainerTM 2 (LMD). In vitro techniques applied were conducted on human keratinocytes to evaluate cell viability (Alamar blue assay) and gene expression real-time reverse transcription−polymerase chain reaction (RT-PCR technique). In addition, it was assessed the irritating effect on the vascular plexus using as a biological model the chorioallantoic membrane of the hen’s egg by applying the hen’s egg-chorioallantoic membrane (HET-CAM) method. The obtained results showed a decrease in cell viability up to 82% in the case of LMD at pH = 3, a slight increase in mRNA expression for the anti-apoptotic marker (Bcl-2 and Bcl-xL), and a decrease in mRNA expression for the pro-apoptotic marker (Bad), and any type of toxic change at the capillary level (irritation score being below 0.9). Based on the data obtained, it can be stated that MB and LMD biological environments, at different pH values, present a safe toxicological profile.
Record ID
Keywords
angiogenic effect, gene expression, keratinocytes, malocclusion, viability
Subject
Suggested Citation
Dinu S, Buzatu R, Macasoi I, Popa M, Vlad CS, Marcovici I, Pinzaru I, Dehelean CA, Moacă EA, Barbu-Tudoran L, Pricop M. Toxicological Profile of Biological Environment of Two Elastodontic Devices. (2023). LAPSE:2023.4180v1
Author Affiliations
Dinu S: Department of Pedodontics, Faculty of Dental Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania; Pediatric Dentistry Research Center, Faculty of Dental Medicine, Victor Babeş Universit [ORCID]
Buzatu R: Department of Dental Aesthetics, Faculty of Dental Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania [ORCID]
Macasoi I: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm
Popa M: Department of Pedodontics, Faculty of Dental Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania; Pediatric Dentistry Research Center, Faculty of Dental Medicine, Victor Babeş Universit
Vlad CS: Department of Pharmacology, Faculty of Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania
Marcovici I: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm
Pinzaru I: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm [ORCID]
Dehelean CA: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm
Moacă EA: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm [ORCID]
Barbu-Tudoran L: Electron Microscopy Laboratory “Prof. C. Craciun”, Faculty of Biology & Geology, “Babes-Bolyai” University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania; Electron Microscopy Integrated Laboratory, National Institute for R&D of Isotopic and M [ORCID]
Pricop M: Department of Maxillofacial Surgery, Faculty of Dental Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania
Buzatu R: Department of Dental Aesthetics, Faculty of Dental Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania [ORCID]
Macasoi I: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm
Popa M: Department of Pedodontics, Faculty of Dental Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania; Pediatric Dentistry Research Center, Faculty of Dental Medicine, Victor Babeş Universit
Vlad CS: Department of Pharmacology, Faculty of Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania
Marcovici I: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm
Pinzaru I: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm [ORCID]
Dehelean CA: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm
Moacă EA: Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania; Departament of Toxicology and Drug Industry, Faculty of Pharm [ORCID]
Barbu-Tudoran L: Electron Microscopy Laboratory “Prof. C. Craciun”, Faculty of Biology & Geology, “Babes-Bolyai” University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania; Electron Microscopy Integrated Laboratory, National Institute for R&D of Isotopic and M [ORCID]
Pricop M: Department of Maxillofacial Surgery, Faculty of Dental Medicine, Victor Babeş University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania
Journal Name
Processes
Volume
9
Issue
12
First Page
2116
Year
2021
Publication Date
2021-11-24
ISSN
2227-9717
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PII: pr9122116, Publication Type: Journal Article
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LAPSE:2023.4180v1
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https://doi.org/10.3390/pr9122116
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