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LAPSE:2023.36635v1

Steam Explosion of Eucalyptus grandis Sawdust for Ethanol Production within a Biorefinery Approach

Mairan Guigou, Juan Guarino, Luana M. Chiarello, María N. Cabrera, Mauricio Vique, Claudia Lareo, Mario D. Ferrari, Luiz P. Ramos

September 20, 2023

Abstract
In this work, Eucalyptus grandis sawdust was subjected to steam explosion as the first step in cellulosic ethanol production within a biorefinery approach. The effect of the moisture content in the eucalypt sawdust (8 and 50%) and pretreatment process variables, such as temperature and residence time, were evaluated along with the influence of the water washing of steam-exploded solids on enzymatic hydrolysis and C6 fermentation yields. All other process streams were characterized to evaluate the recovery yield of valuable co-products. A recovery of nearly 100% glucans in the solid fraction and 60% xylans in the liquid fraction, mainly as partially acetylated oligomers, was obtained. The best enzymatic hydrolysis efficiencies (66−67%) were achieved after pretreatment at 205 °C for 10 min. The washing of pretreated sawdust with water improved the hydrolysis efficiencies and ethanol production yields by 10% compared to the unwashed pretreated solids under the same experimental condition. The highest ethanol yields were achieved after pretreatment of the sawdust with an 8% moisture content at 205 °C for 10 min, enzymatic hydrolysis at 13 wt% total solids with 25 FPU/g glucans, and fermentation with S. cerevisiae PE-2. In this case, 227 L ethanol and 40 kg total xylose (including xylo-oligomers) were obtained per ton of dry eucalypt sawdust.

Record ID

LAPSE:2023.36635v1

Keywords

biomass moisture content, cellulosic ethanol, eucalypt sawdust, high total solids, PSSF, steam explosion

Subject

Food & Agricultural Processes

Suggested Citation

Guigou M, Guarino J, Chiarello LM, Cabrera MN, Vique M, Lareo C, Ferrari MD, Ramos LP. Steam Explosion of Eucalyptus grandis Sawdust for Ethanol Production within a Biorefinery Approach. (2023). LAPSE:2023.36635v1

Author Affiliations

Guigou M: Departamento de Bioingeniería, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, J. Herrera y Reissig 565, Montevideo CP 11300, Uruguay
Guarino J: Grupo de Ingeniería de Procesos Forestales, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, J. Herrera y Reissig 565, Montevideo CP 11300, Uruguay
Chiarello LM: Graduate Program in Chemical Engineering, Federal University of Paraná, P.O. Box 19032, Curitiba 81531-980, PR, Brazil [ORCID]
Cabrera MN: Grupo de Ingeniería de Procesos Forestales, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, J. Herrera y Reissig 565, Montevideo CP 11300, Uruguay [ORCID]
Vique M: Departamento de Bioingeniería, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, J. Herrera y Reissig 565, Montevideo CP 11300, Uruguay
Lareo C: Departamento de Bioingeniería, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, J. Herrera y Reissig 565, Montevideo CP 11300, Uruguay [ORCID]
Ferrari MD: Departamento de Bioingeniería, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, J. Herrera y Reissig 565, Montevideo CP 11300, Uruguay
Ramos LP: Graduate Program in Chemical Engineering, Federal University of Paraná, P.O. Box 19032, Curitiba 81531-980, PR, Brazil [ORCID]

Journal Name

Processes

Volume

Issue

First Page

2277

Year

2023

Publication Date

2023-07-28

DOI:

https://doi.org/10.3390/pr11082277