Microchannel cooling technology is an effective method to solve local thermal stacking. In this paper, four innovative microchannels with bionic fish-shaped rib arrangements (CM-O, CM-R, CM-H, and CM-G) are designed by imitating geese and fish clusters. The heat transfer and flow characteristics of the microchannels are simulated numerically at different Reynold’s numbers (Re = 200 − 1600). The liquid water temperature and flow field in the four innovative microchannels with bionic ribs are analyzed. The results show that the ribs’ arrangement has an influence on the thermal performance of microchannels. Compared to the smooth microchannel (CM), the of the Nu microchannels with the bionic fish-shaped ribs increases by 33.00−53.26% while the fave increases by 28.63−34.93% at Re = 1200. The vortices around the ribs are clearly observed which improves the temperature gradient. The performance evaluation criterion (PEC) of CM-H is higher than that of the others. This indicates that the rib arrangement of CM-H is superior for heat dissipation application.