LAPSE:2023.36122
Published Article
LAPSE:2023.36122
Digital Twin Based Design and Experimental Validation of a Continuous Peptide Polishing Step
June 13, 2023
Optimizing or debottlenecking existing production plants is a challenging task. In this case study, an existing reversed phased chromatography polishing step for peptide purification was optimized with the help of a digital twin. The existing batch chromatography was depicted digitally with the general rate model. Model parameter determination and model validation was done with dedicated experiments. The digital twin was then used to identify optimized process variants, especially continuous chromatography steps. MCSGP was found to achieve high purities and yield but at the cost of productivity due to column synchronization. An alternative Continuous Twin Column chromatography process (CTCC) was established that eliminates unnecessary waiting times. Ensuring the same or higher purity compared to the batch process, the continuous process achieved a yield increase of 31% and productivity increase of 27.6%. Experimental long runs confirmed these results.
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Keywords
continuous chromatography, digital twin, peptide purification
Subject
Suggested Citation
Zobel-Roos S, Vetter F, Scheps D, Pfeiffer M, Gunne M, Boscheinen O, Strube J. Digital Twin Based Design and Experimental Validation of a Continuous Peptide Polishing Step. (2023). LAPSE:2023.36122
Author Affiliations
Zobel-Roos S: Institute for Separation and Process Technology, Clausthal University of Technology, Leibnizstraße 15, D-38678 Clausthal-Zellerfeld, Germany
Vetter F: Institute for Separation and Process Technology, Clausthal University of Technology, Leibnizstraße 15, D-38678 Clausthal-Zellerfeld, Germany
Scheps D: CMC Microbial Platform, Sanofi-Aventis Deutschland GmbH, D-65926 Frankfurt am Main, Germany
Pfeiffer M: CMC Microbial Platform, Sanofi-Aventis Deutschland GmbH, D-65926 Frankfurt am Main, Germany
Gunne M: IA MSAT M&I DS, Sanofi-Aventis Deutschland GmbH, D-65926 Frankfurt am Main, Germany [ORCID]
Boscheinen O: CMC Microbial Platform, Sanofi-Aventis Deutschland GmbH, D-65926 Frankfurt am Main, Germany [ORCID]
Strube J: Institute for Separation and Process Technology, Clausthal University of Technology, Leibnizstraße 15, D-38678 Clausthal-Zellerfeld, Germany
Vetter F: Institute for Separation and Process Technology, Clausthal University of Technology, Leibnizstraße 15, D-38678 Clausthal-Zellerfeld, Germany
Scheps D: CMC Microbial Platform, Sanofi-Aventis Deutschland GmbH, D-65926 Frankfurt am Main, Germany
Pfeiffer M: CMC Microbial Platform, Sanofi-Aventis Deutschland GmbH, D-65926 Frankfurt am Main, Germany
Gunne M: IA MSAT M&I DS, Sanofi-Aventis Deutschland GmbH, D-65926 Frankfurt am Main, Germany [ORCID]
Boscheinen O: CMC Microbial Platform, Sanofi-Aventis Deutschland GmbH, D-65926 Frankfurt am Main, Germany [ORCID]
Strube J: Institute for Separation and Process Technology, Clausthal University of Technology, Leibnizstraße 15, D-38678 Clausthal-Zellerfeld, Germany
Journal Name
Processes
Volume
11
Issue
5
First Page
1401
Year
2023
Publication Date
2023-05-05
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr11051401, Publication Type: Journal Article
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Published Article
LAPSE:2023.36122
This Record
External Link
doi:10.3390/pr11051401
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Version History
[v1] (Original Submission)
Jun 13, 2023
Verified by curator on
Jun 13, 2023
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v1
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https://psecommunity.org/LAPSE:2023.36122
Original Submitter
Calvin Tsay
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