In order to analyze the inner flow in a multi-stage double-suction centrifugal pump, which is regarded as a common way of knowing the current characteristics of the pump and as the basis of optimization for better performance, a numerical simulation considering the velocity field distribution characteristics and pressure fluctuation propagation law using the detached eddy simulation method was conducted. Additionally, the principle of entropy generation was put to use to quantify and compare the energy loss of different components. The results reveal that the existence of unstable flow structures in the first-stage impeller and a large number of vortical structures in the back-channel result in reduced operational efficiency of the pump. Furthermore, the pressure fluctuation intensity reaches its maximum with 0.15 at the blade trailing edge, which propagates to the tongue region of the forward flow channel and the double-volute under the low rates condition. Additionally, the main frequency of the monitoring points in the inter-stage flow channel and volute is basically located at a frequency of 198.667 Hz, which is twice the blade frequency. Consequently, the wall entropy production accounting for nearly 25% cannot be ignored and that the loss mainly occurs in the double-volute and the inter-stage flow channel due to the occurrence of irregular flow in the above components with more than 50%. The outcomes of this research present a valuable point of reference for the optimization of structural design in multistage turbomachines with various applications.