LAPSE:2023.3281
Published Article
LAPSE:2023.3281
Production of Non-Volatile Metabolites from Sooty Molds and Their Bio-Functionalities
February 22, 2023
Abstract
In the current study, eleven sooty mold isolates were collected from different tropical host plants. The isolates were identified under Capnodium, Leptoxyphium and Trichomerium, based on morphology and phylogeny. For the secondary metabolite analysis, the isolates were grown on Potato Dextrose Broth (PDB). The well-grown mycelia were filtered and extracted over methanol (MeOH). The metabolites in the growth medium (or filtrate) were extracted over ethyl acetate (EtOAc). The antifungal activities of each crude extract were tested over Alternaria sp., Colletotrichum sp., Curvularia sp., Fusarium sp. and Pestalotiopsis sp. The metabolites were further tested for their total phenolic, flavonoid and protein content prior to their antioxidant and anti-fungal potential evaluation. The MeOH extracts of sooty molds were enriched with proteins and specifically inhibited Curvularia sp. The total phenolic content and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activity was largely recovered from the filtrate corresponding to the inhibition of Alternaria sp.; while the flavonoid and free radical reduction suggested a relative induction of growth of the Fusarium sp., Colletotrichum sp. and Pestalotiopsis sp. Hence, this study reveals the diversity of sooty molds in Thailand by a modern phylogenetic approach. Furthermore, the preliminary screening of the isolates reveals the potential of finding novel compounds and providing insights for the future research on secondary metabolites of bio-trophic fungi and their potential usage on sustainable agriculture.
In the current study, eleven sooty mold isolates were collected from different tropical host plants. The isolates were identified under Capnodium, Leptoxyphium and Trichomerium, based on morphology and phylogeny. For the secondary metabolite analysis, the isolates were grown on Potato Dextrose Broth (PDB). The well-grown mycelia were filtered and extracted over methanol (MeOH). The metabolites in the growth medium (or filtrate) were extracted over ethyl acetate (EtOAc). The antifungal activities of each crude extract were tested over Alternaria sp., Colletotrichum sp., Curvularia sp., Fusarium sp. and Pestalotiopsis sp. The metabolites were further tested for their total phenolic, flavonoid and protein content prior to their antioxidant and anti-fungal potential evaluation. The MeOH extracts of sooty molds were enriched with proteins and specifically inhibited Curvularia sp. The total phenolic content and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activity was largely recovered from the filtrate corresponding to the inhibition of Alternaria sp.; while the flavonoid and free radical reduction suggested a relative induction of growth of the Fusarium sp., Colletotrichum sp. and Pestalotiopsis sp. Hence, this study reveals the diversity of sooty molds in Thailand by a modern phylogenetic approach. Furthermore, the preliminary screening of the isolates reveals the potential of finding novel compounds and providing insights for the future research on secondary metabolites of bio-trophic fungi and their potential usage on sustainable agriculture.
Record ID
Keywords
antifungal activity, cell-wall lytic enzyme, flavonoids, fungal extracts, fungal secondary metabolites, phenolic compounds, phylogeny, taxonomy
Subject
Suggested Citation
Haituk S, Withee P, Sangta J, Senwanna C, Khamsaw P, Karunarathna A, Hongsibsong S, Sringarm K, Prasad SK, Sommano SR, Cheewangkoon R. Production of Non-Volatile Metabolites from Sooty Molds and Their Bio-Functionalities. (2023). LAPSE:2023.3281
Author Affiliations
Haituk S: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Withee P: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Sangta J: Plant Bioactive Compound Laboratory, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Senwanna C: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Khamsaw P: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Karunarathna A: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Hongsibsong S: School of Health Science Research, Chiang Mai University, Chiang Mai 50200, Thailand; Research Institute for Health Science, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Sringarm K: Department of Animal and Aquatic Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Prasad SK: Department of Biotechnology and Bioinformatics, Faculty of Life Sciences, JSS Academy of Higher Education and Research (JSSAHER), Mysuru 570015, Karnataka, India [ORCID]
Sommano SR: Plant Bioactive Compound Laboratory, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Cheewangkoon R: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; Innovative Agriculture Research Centre, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of
Withee P: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Sangta J: Plant Bioactive Compound Laboratory, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Senwanna C: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Khamsaw P: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Karunarathna A: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Hongsibsong S: School of Health Science Research, Chiang Mai University, Chiang Mai 50200, Thailand; Research Institute for Health Science, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Sringarm K: Department of Animal and Aquatic Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Prasad SK: Department of Biotechnology and Bioinformatics, Faculty of Life Sciences, JSS Academy of Higher Education and Research (JSSAHER), Mysuru 570015, Karnataka, India [ORCID]
Sommano SR: Plant Bioactive Compound Laboratory, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand [ORCID]
Cheewangkoon R: Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; Innovative Agriculture Research Centre, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of
Journal Name
Processes
Volume
10
Issue
2
First Page
329
Year
2022
Publication Date
2022-02-08
ISSN
2227-9717
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PII: pr10020329, Publication Type: Journal Article
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LAPSE:2023.3281
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https://doi.org/10.3390/pr10020329
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