Biogas production is one of the solutions for replacing fossil fuels, which promotes the widespread use of green energy. The aim of this study was to determine the potential of Silphium perfoliatum as an energy crop for biogas production, as well as the effect of different fertilization doses (0, 85 and 170 kg N ha−1) on the production potential (NL CH4 kg−1 VS) of Silphium perfoliatum. The study investigated the use of different feedstocks, such as raw and ensiled Silphium perfoliatum biomass. The methane production ranged between 193.59 and 243.61 NL CH4 kg−1 VS. The highest biogas production potential was achieved with the biomasses which were cultivated with the highest fertilization dose (170 kg N ha−1), both for raw and ensiled crop biomasses, although the difference from the other fertilization doses was not significant. The feedstock (biomass and silage) and digestate parameters were investigated as well. The use of Silphium perfoliatum for biogas production seems very promising since its methane production potential was found to be similar to that of the most common energy crop, such as maize, indicating that Silphium perfoliatum can compete in the future with maize.