Turbulent flow, mainly originating from the rotor-stator interaction (RSI), is closely associated with the normal and safe operation of the centrifugal pump. In the current research, to clarify turbulent flow in the centrifugal pump with a vaned diffuser, the non-intrusive LDA (Laser Doppler Anemometry) system is applied to measure velocity pulsation signals at different regions when the pump operates at various flow rates. Time and frequency domain analysis methods are combined to investigate the velocity signals, and the velocity distribution around the volute tongue region is reconstructed from twenty measuring points. Results show that the velocity spectrum is characterized by the discrete components at the blade passing frequency and its higher harmonics, and it is caused by the RSI between the impeller and the diffuser. For the points in the volute spiral and diffusion sections, due to the significantly reduced RSI effect, the velocity spectrum shows an evident difference from comparison with the points between the impeller and diffuser, and the blade passing frequency is not always the dominant frequency. The comparison of velocity amplitudes and RMS* (root mean square of velocity) values at different points proves that the measuring position and flow rate affect velocity pulsations. As observed from velocity distribution reconstructed by LDA signals, high velocity regions are developed downstream of the diffuser channel for all the measured flow rates.