Abstract
Hydrolysate prepared from the chemical hydrolysis of water hyacinth biomass contains a high amount of solubilised carbohydrate and nutrients. This hydrolysate was utilised as a medium for the cultivation of two strains of Chlorella sorokiniana, isolated from a municipal wastewater treatment plant using two different media, i.e., BG-11 and Knop’s medium. Different light intensities, light−dark cycles, and various concentrations of external carbon sources (monosaccharides and inorganic carbon) were used to optimise the microalgal growth. For the accumulation of lipids and carbohydrates, the microalgal strains were transferred to nutrient amended medium (N-amended and P-amended). It was observed that the combined effect of glucose, inorganic carbon, and a 12:12 h light−dark cycle proved to be the optimum parameters for high biomass productivity (~200 mg/L/day). For Chlorella sorokiniana 1 (isolated from BG-11 medium), the maximum carbohydrate content (22%) was found in P-amended medium (N = 0 mg/L, P: 3 mg/L), whereas, high lipid content (17.3%) was recorded in N-amended medium (N = 5 mg/L, P = 0 mg/L). However, for Chlorella sorokiniana 2 (isolated from the Knop’s medium), both lipid (17%) and carbohydrate accumulation (12.3%) were found to be maximum in the N-amended medium. Chlorella sorokiniana 2 showed a high saturated lipid accumulation compared to other strains. Kinetic modelling of the lipid profile revealed that the production rate of fatty acids and their various constituents were species dependent under identical conditions.