LAPSE:2023.29792
Published Article
LAPSE:2023.29792
The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure
April 13, 2023
This study explored the effect of eight antimicrobials on the efficiency of biogas production in the anaerobic digestion (AD) process of cattle manure. The microbiome involved in AD, presence and number of genes mcrA, MSC and MST specific for Archaea, and antibiotic resistance genes (ARGs) concentration in digestate (D) were examined. Supplementation of antibiotics to substrate significantly lowered biogas production. Amoxicillin caused a 75% decrease in CH4 production in comparison with the control samples. Enrofloxacin, tetracycline, oxytetracycline, and chlortetracycline reduced the amount of biogas produced by 36, 39, 45 and 53%, respectively. High-throughput sequencing of 16S rRNA results revealed that bacteria dominated the Archaea microorganisms in all samples. Moreover, antibiotics led to a decrease in the abundance of the genes mcrA, MSC, MST, and induced an increase in the number of tetracyclines resistance genes. Antibiotics decreased the efficiency of the AD process and lowered the quantity of CH4 obtained, while stimulating an increase in the number of ARGs in D. This work reveals how antimicrobials affect the cattle manure AD process and changes in microbial biodiversity, number of functional genes and ARGs in the digestate due to drugs exposure. It also, provides useful, practical information about the AD process.
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Keywords
antibiotic resistance genes, biogas production, Illumina MiSeq Sequencing, mesophilic anaerobic digestion, microbiome biodiversity
Subject
Suggested Citation
Koniuszewska I, Harnisz M, Korzeniewska E, Czatzkowska M, Jastrzębski JP, Paukszto Ł, Bajkacz S, Felis E, Rusanowska P. The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure. (2023). LAPSE:2023.29792
Author Affiliations
Koniuszewska I: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland
Harnisz M: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland [ORCID]
Korzeniewska E: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland [ORCID]
Czatzkowska M: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland [ORCID]
Jastrzębski JP: Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-957 Olsztyn, Poland [ORCID]
Paukszto Ł: Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-957 Olsztyn, Poland [ORCID]
Bajkacz S: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland; Environmental Biotechnology Department, Faculty of Energy and Environmental Engineeri
Felis E: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland; The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-
Rusanowska P: Department of Environmental Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Warszawska 117 Str., 10-950 Olsztyn, Poland [ORCID]
Harnisz M: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland [ORCID]
Korzeniewska E: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland [ORCID]
Czatzkowska M: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland [ORCID]
Jastrzębski JP: Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-957 Olsztyn, Poland [ORCID]
Paukszto Ł: Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-957 Olsztyn, Poland [ORCID]
Bajkacz S: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland; Environmental Biotechnology Department, Faculty of Energy and Environmental Engineeri
Felis E: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland; The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-
Rusanowska P: Department of Environmental Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Warszawska 117 Str., 10-950 Olsztyn, Poland [ORCID]
Journal Name
Energies
Volume
14
Issue
4
First Page
1125
Year
2021
Publication Date
2021-02-20
Published Version
ISSN
1996-1073
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PII: en14041125, Publication Type: Journal Article
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LAPSE:2023.29792
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doi:10.3390/en14041125
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