LAPSE:2023.2944v1
Published Article
LAPSE:2023.2944v1
Manno-Oligosaccharide Production from Biomass Hydrolysis by Using Endo-1,4-β-Mannanase (ManNj6-379) from Nonomuraea jabiensis ID06-379
February 21, 2023
Abstract
A novel endo-β-1,4-mannanase gene was cloned from a novel actinomycetes, Nonomuraea jabiensis ID06-379, isolated from soil, overexpressed as an extracellular protein (47.8 kDa) in Streptomyces lividans 1326. This new endo-1,4-β-mannanase gene (manNj6-379) is encoded by 445-amino acids. The ManNj6-379 consists of a 28-residue signal peptide and a carbohydrate-binding module of family 2 belonging to the glycoside hydrolase (GH) family 5, with 59−77% identity to GH5 mannan endo-1,4-β-mannanase. The recombinant ManNj6-379 displayed an optimal pH of 6.5 with pH stability ranging between 5.5 and 7.0 and was stable for 120 min at 50 °C and lower temperatures. The optimal temperature for activity was 70 °C. An enzymatic hydrolysis assay revealed that ManNj6-379 could hydrolyze commercial β-mannan and biomass containing mannan.
A novel endo-β-1,4-mannanase gene was cloned from a novel actinomycetes, Nonomuraea jabiensis ID06-379, isolated from soil, overexpressed as an extracellular protein (47.8 kDa) in Streptomyces lividans 1326. This new endo-1,4-β-mannanase gene (manNj6-379) is encoded by 445-amino acids. The ManNj6-379 consists of a 28-residue signal peptide and a carbohydrate-binding module of family 2 belonging to the glycoside hydrolase (GH) family 5, with 59−77% identity to GH5 mannan endo-1,4-β-mannanase. The recombinant ManNj6-379 displayed an optimal pH of 6.5 with pH stability ranging between 5.5 and 7.0 and was stable for 120 min at 50 °C and lower temperatures. The optimal temperature for activity was 70 °C. An enzymatic hydrolysis assay revealed that ManNj6-379 could hydrolyze commercial β-mannan and biomass containing mannan.
Record ID
Keywords
cloning, endo-1,4-β-mannanase gene, Nonomuraea jabiensis ID06-379, Streptomyces lividans 1326
Subject
Suggested Citation
Ratnakomala S, Kahar P, Kashiwagi N, Lee J, Kudou M, Matsumoto H, Apriliana P, Yopi Y, Prasetya B, Ogino C, Kondo A. Manno-Oligosaccharide Production from Biomass Hydrolysis by Using Endo-1,4-β-Mannanase (ManNj6-379) from Nonomuraea jabiensis ID06-379. (2023). LAPSE:2023.2944v1
Author Affiliations
Ratnakomala S: Research Center for Biology, National Research and Innovation Agency, Cibinong Science Center, Cibinong 16911, Indonesia [ORCID]
Kahar P: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan [ORCID]
Kashiwagi N: Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan
Lee J: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
Kudou M: Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan
Matsumoto H: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
Apriliana P: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
Yopi Y: The National Standardization Agency of Indonesia (BSN), Research Center and HRD, South Tangerang 15314, Indonesia
Prasetya B: The National Standardization Agency of Indonesia (BSN), Research Center and HRD, South Tangerang 15314, Indonesia
Ogino C: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
Kondo A: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan; Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan
Kahar P: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan [ORCID]
Kashiwagi N: Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan
Lee J: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
Kudou M: Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan
Matsumoto H: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
Apriliana P: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
Yopi Y: The National Standardization Agency of Indonesia (BSN), Research Center and HRD, South Tangerang 15314, Indonesia
Prasetya B: The National Standardization Agency of Indonesia (BSN), Research Center and HRD, South Tangerang 15314, Indonesia
Ogino C: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan
Kondo A: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan; Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan
Journal Name
Processes
Volume
10
Issue
2
First Page
269
Year
2022
Publication Date
2022-01-29
ISSN
2227-9717
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PII: pr10020269, Publication Type: Journal Article
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LAPSE:2023.2944v1
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https://doi.org/10.3390/pr10020269
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