Recovery of Anthocyanins from Hibiscus sabdariffa L. Using a Combination of Supercritical Carbon Dioxide Extraction and Subcritical Water Extraction

Dwila Nur Rizkiyah; Nicky Rahmana Putra; Zuhaili Idham; Ahmad Hazim Abdul Aziz; Mohd Azizi Che Yunus; Ibham Veza; Irianto; Stanley Chinedu Mamah; Lailatul Qomariyah

LAPSE:2023.28195v1

Published Article

LAPSE:2023.28195v1

Recovery of Anthocyanins from Hibiscus sabdariffa L. Using a Combination of Supercritical Carbon Dioxide Extraction and Subcritical Water Extraction

Dwila Nur Rizkiyah, Nicky Rahmana Putra, Zuhaili Idham, Ahmad Hazim Abdul Aziz, Mohd Azizi Che Yunus, Ibham Veza, Irianto, Stanley Chinedu Mamah, Lailatul Qomariyah

April 11, 2023

Abstract
Anthocyanins are one of the bioactive compounds in roselle that has many medicinal proposes. Anthocyanins are placed in the inner part of the roselle; therefore, combinations of two methods were applied to extract the anthocyanins. The first stage is employing supercritical carbon dioxide (ScCO2) to break the particle surface or outer layer of the roselle based on the total phenolic compounds (TPC) recovery, and the second step was to apply subcritical water extraction (SWE) for the extraction of anthocyanins. The objective is to determine the best conditions to obtain high yields of total anthocyanins compounds (TAC) from the roselle (Hibiscus sabdariffa) by employing a combination of ScCO2 and SWE. The optimal conditions of ScCO2 (first stage) were 19.13 MPa, 60 °C, and 4.31 mL/min, yielding 18.20%, and 80.34 mg/100 g TPC, respectively. The optimum conditions of SWE (second stage) were 9.48 MPa, 137 °C, and 6.14 mL/min, yielding 86.11% and 1224.61 mg/100 g TAC, respectively. The application of integrated ScCO2 and SWE proved successful in achieving high anthocyanins production and yield as compared to previous extraction methods. This approach may be used to extract the roselle with a greater anthocyanin’s concentration than the prior method.

Record ID

LAPSE:2023.28195v1

Keywords

anthocyanins, Optimization, roselle, subcritical water, supercritical carbon dioxide

Subject

Optimization

Suggested Citation

Rizkiyah DN, Putra NR, Idham Z, Aziz AHA, Che Yunus MA, Veza I, Irianto, Chinedu Mamah S, Qomariyah L. Recovery of Anthocyanins from Hibiscus sabdariffa L. Using a Combination of Supercritical Carbon Dioxide Extraction and Subcritical Water Extraction. (2023). LAPSE:2023.28195v1

Author Affiliations

Rizkiyah DN: Centre of Lipid Engineering and Applied Research (CLEAR), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, UTM Johor Bahru Campus, Johor Bahru 81310, Malaysia
Putra NR: Centre of Lipid Engineering and Applied Research (CLEAR), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, UTM Johor Bahru Campus, Johor Bahru 81310, Malaysia [ORCID]
Idham Z: Centre of Lipid Engineering and Applied Research (CLEAR), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, UTM Johor Bahru Campus, Johor Bahru 81310, Malaysia
Aziz AHA: Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Kota Kinabalu 88400, Malaysia [ORCID]
Che Yunus MA: Centre of Lipid Engineering and Applied Research (CLEAR), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, UTM Johor Bahru Campus, Johor Bahru 81310, Malaysia [ORCID]
Veza I: Department of Mechanical Engineering, Universiti Teknologi PETRONAS, Seri Iskandar 32610, Malaysia [ORCID]
Irianto: Department General Education, Faculty of Resillience, Rabdan Academy, Abu Dhabi P.O. Box 114646, United Arab Emirates [ORCID]
Chinedu Mamah S: Chemical Engineering Department, Alex Ekwueme Federal University Ndufu-Alike, Abakaliki P.O. Box 1010, Ebonyi State, Nigeria [ORCID]
Qomariyah L: Department of Industrial Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia

Journal Name

Processes

Volume

11

Issue

3

First Page

751

Year

2023

Publication Date

2023-03-03