Renewable energy production is gaining importance in the context of global climate changes. However, in some countries other aspects increasing the role of renewable energy production are also present. Such a country is Ukraine, which is not self-sufficient in energy supply and whose dependency on poorly diversified import of energy carriers regularly leads to political tensions and has socio-economic implications. Production of agricultural biogas seems to be a way to both slow down climatic changes and increase energy self-sufficiency by replacing or complementing conventional sources of energy. One of the most substantial barriers to agricultural biogas production is the low level of agricultural concentration and significant economies of scale in constructing biogas plants. The aim of the paper was thus to assess the potential of agricultural biogas production in Ukraine, including its impact on energy self-sufficiency, mitigation of greenhouse gas (GHG) emissions and the economic performance of biogas plants. The results show that due to the prevailing fragmentation of farms, most manure cannot be processed in an economically viable way. However, in some regions utilization of technically available manure for agricultural biogas production could cover up to 11% of natural gas or up to 19% of electricity demand. While the theoretical potential for reducing greenhouse gas emissions could reach 5% to 6.14%, the achievable technical potential varies between 2.3% and 2.8% of total emissions. The economic performance of agricultural biogas plants correlates closely with their size and bioenergy generation potential.