Abstract
As important parameters in the characterization of aerobic granulation, the shape and average diameter were related to substrates. The previous studies disclosed that the morphology change in aerobic granules was the result of growth and the relatively strong hydrodynamic shear force. No further exploration of the size distribution of the aerobic granules has been conducted. To better understand the impact of toxic compounds on aerobic granules’ growth during their formation, the properties of aerobic granules were traced over 81 days in 3 sequencing batch reactors fed with acetate and berberine wastewater, especially the particle size and size distribution. The results showed that the aerobic granules were cultivated by the simulated acetate wastewater (R1), simulated berberine wastewater (R2), and effluent from an anaerobic baffled reactor (ABR) reactor which was fed with industrial berberine wastewater (R3). The reactors exhibited different COD removal efficiencies, and the MLSS and MLVSS values affected by the different substrates which were in an order of R1 > R2 > R3. However, the SVI and SOUR, which were affected by several factors, showed more complicated results. The aerobic granules had the lowest microbial activity (SOUR), while the aerobic granules in R3 had the lowest settling ability among the three kinds of granules. For the three reactors with different influent compositions, the aerobic granulation process displayed a three-stage process separately. Compared with the granules fed with berberine wastewater, the granules fed with acetate in a stable operation period showed more independence from other periods.The size distribution was affected by substrates. The aerobic granules with a range of 0.3−1.0 µm occupied 77.0%, 67.0%, and 35.7% of the volume for R1, R2, and R3, respectively. The biomass less than 0.3 µm occupied 59.1% volume in R3. The components of the substrate had a great influence on the growth of aerobic granules, not only on the diameter but also on the size distribution.