LAPSE:2023.22791v1
Published Article
LAPSE:2023.22791v1
The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes
March 24, 2023
Abstract
Ozone is an allotropic form of oxygen, so in the medical field ozone therapy has special effects. Starting from the premise that bio-oxidative ozone therapy reduces the number of bacteria, in the present study two approaches were proposed: to evaluate the biological effects of ozone gas on the tooth enamel remineralization process and to demonstrate its impact on the morphology and confluence of human primary gingival cells, namely keratinocytes (PGK) and fibroblasts (HGF). The ozone produced by HealOzone was applied in vivo to 68 M1s (first permanent molars), both maxillary and mandibular, on the occlusal surfaces at pit and fissure. The molars included in the study recorded values between 13 and 24 according to the DIAGNOdent Pen 2190 scale, this being the main inclusion/exclusion criterion for the investigated molars. Because the gas can make contact with primary gingival cells during the ozonation process, both human gingival fibroblasts and keratinocytes were exposed to different doses of ozone (20 s, 40 s, 60 s), and its effects were observed with the Olympus IX73 inverted microscope. The contact of ozone with the human primary gingival cells demonstrates cell sensitivity to the action of ozone, this being higher in fibroblasts compared to keratinocytes, but it is not considered toxic because all the changes are reversible at 48 h after exposure.
Ozone is an allotropic form of oxygen, so in the medical field ozone therapy has special effects. Starting from the premise that bio-oxidative ozone therapy reduces the number of bacteria, in the present study two approaches were proposed: to evaluate the biological effects of ozone gas on the tooth enamel remineralization process and to demonstrate its impact on the morphology and confluence of human primary gingival cells, namely keratinocytes (PGK) and fibroblasts (HGF). The ozone produced by HealOzone was applied in vivo to 68 M1s (first permanent molars), both maxillary and mandibular, on the occlusal surfaces at pit and fissure. The molars included in the study recorded values between 13 and 24 according to the DIAGNOdent Pen 2190 scale, this being the main inclusion/exclusion criterion for the investigated molars. Because the gas can make contact with primary gingival cells during the ozonation process, both human gingival fibroblasts and keratinocytes were exposed to different doses of ozone (20 s, 40 s, 60 s), and its effects were observed with the Olympus IX73 inverted microscope. The contact of ozone with the human primary gingival cells demonstrates cell sensitivity to the action of ozone, this being higher in fibroblasts compared to keratinocytes, but it is not considered toxic because all the changes are reversible at 48 h after exposure.
Record ID
Keywords
dental remineralization, ozone, primary gingival fibroblasts, primary gingival keratinocytes
Subject
Suggested Citation
Floare AD, Scurtu AD, Balean OI, Chioran D, Buzatu R, Sava Rosianu R, Alexa VT, Jumanca D, Rusu LC, Racea RC, Coricovac D, Pinzaru I, Dehelean CA, Galuscan A. The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes. (2023). LAPSE:2023.22791v1
Author Affiliations
Floare AD: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Scurtu AD: Department of Toxicology, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine [ORCID]
Balean OI: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Translational and Experimental Clinical Research Center in Oral Health (TEXC-OH), 14A Tudor Vladimir [ORCID]
Chioran D: Department II, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Buzatu R: Department II, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania [ORCID]
Sava Rosianu R: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Translational and Experimental Clinical Research Center in Oral Health (TEXC-OH), 14A Tudor Vladimir [ORCID]
Alexa VT: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania [ORCID]
Jumanca D: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Translational and Experimental Clinical Research Center in Oral Health (TEXC-OH), 14A Tudor Vladimir [ORCID]
Rusu LC: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Vict [ORCID]
Racea RC: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Vict
Coricovac D: Department of Toxicology, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine [ORCID]
Pinzaru I: Department of Toxicology, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine [ORCID]
Dehelean CA: Department of Toxicology, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine
Galuscan A: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Translational and Experimental Clinical Research Center in Oral Health (TEXC-OH), 14A Tudor Vladimir
Scurtu AD: Department of Toxicology, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine [ORCID]
Balean OI: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Translational and Experimental Clinical Research Center in Oral Health (TEXC-OH), 14A Tudor Vladimir [ORCID]
Chioran D: Department II, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Buzatu R: Department II, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania [ORCID]
Sava Rosianu R: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Translational and Experimental Clinical Research Center in Oral Health (TEXC-OH), 14A Tudor Vladimir [ORCID]
Alexa VT: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania [ORCID]
Jumanca D: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Translational and Experimental Clinical Research Center in Oral Health (TEXC-OH), 14A Tudor Vladimir [ORCID]
Rusu LC: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Vict [ORCID]
Racea RC: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Vict
Coricovac D: Department of Toxicology, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine [ORCID]
Pinzaru I: Department of Toxicology, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine [ORCID]
Dehelean CA: Department of Toxicology, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine
Galuscan A: Departament I, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania; Translational and Experimental Clinical Research Center in Oral Health (TEXC-OH), 14A Tudor Vladimir
Journal Name
Processes
Volume
9
Issue
11
First Page
1978
Year
2021
Publication Date
2021-11-05
ISSN
2227-9717
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PII: pr9111978, Publication Type: Journal Article
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LAPSE:2023.22791v1
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