One-Step or Two-Step Acid/Alkaline Pretreatments to Improve Enzymatic Hydrolysis and Sugar Recovery from Arundo Donax L.

Shangyuan Tang; Yushen Cao; Chunming Xu; Yue Wu; Lingci Li; Peng Ye; Ying Luo; Yifan Gao; Yonghong Liao; Qiong Yan; Xiyu Cheng

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2023.21720

Published Article

LAPSE:2023.21720

One-Step or Two-Step Acid/Alkaline Pretreatments to Improve Enzymatic Hydrolysis and Sugar Recovery from Arundo Donax L.

Shangyuan Tang, Yushen Cao, Chunming Xu, Yue Wu, Lingci Li, Peng Ye, Ying Luo, Yifan Gao, Yonghong Liao, Qiong Yan, Xiyu Cheng

March 22, 2023

Energy crops are not easily converted by microorganisms because of their recalcitrance. This necessitates a pretreatment to improve their biodigestibility. The effects of different pretreatments, as well as their combination on the enzymatic digestibility of Arundo donax L. were systematically investigated to evaluate its potential for bioconversion. Dilute alkaline pretreatment (ALP) using 1.2% NaOH at 120 °C for 30 min resulted in the highest reducing sugar yield in the enzymatic hydrolysis process because of its strong delignification and morphological modification, while ferric chloride pretreatment (FP) was effective in removing hemicellulose and recovering soluble sugars in the pretreatment stage. Furthermore, an efficient two-step ferric chloride-alkaline pretreatment (FALP) was successfully developed. In the first FP step, easily degradable cellulosic components, especially hemicellulose, were dissolved and then effectively recovered as soluble sugars. Subsequently, the FP sample was further treated in the second ALP step to remove lignin to enhance the enzymatic hydrolysis of the hardly degradable cellulose. As a result, the integrated two-step process obtained the highest total sugar yield of 420.4 mg/g raw stalk in the whole pretreatment and enzymatic hydrolysis process; hence, the process is a valuable candidate for biofuel production.

Record ID

LAPSE:2023.21720

Keywords

Arundo donax L., dilute alkaline pretreatment, enzymatic hydrolysis, ferric chloride pretreatment, two-step process

Subject

Biosystems

Suggested Citation

Tang S, Cao Y, Xu C, Wu Y, Li L, Ye P, Luo Y, Gao Y, Liao Y, Yan Q, Cheng X. One-Step or Two-Step Acid/Alkaline Pretreatments to Improve Enzymatic Hydrolysis and Sugar Recovery from Arundo Donax L.. (2023). LAPSE:2023.21720

Author Affiliations

Tang S: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
Cao Y: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
Xu C: Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China
Wu Y: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
Li L: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
Ye P: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
Luo Y: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
Gao Y: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
Liao Y: Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China
Yan Q: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
Cheng X: College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China [ORCID]

Journal Name

Energies

Volume

13

Issue

4

Article Number

E948

Year

2020

Publication Date

2020-02-20