LAPSE:2023.21160
Published Article
LAPSE:2023.21160
Optimization of Batch Dark Fermentation of Chlorella sp. Using Mixed-Cultures for Simultaneous Hydrogen and Butyric Acid Production
March 21, 2023
Abstract
This paper reports on the optimum conditions for simultaneous hydrogen and butyric acid production from microalgae (Chlorella sp.) using enriched anaerobic mixed cultures as inoculum. The fermentation was objectively carried out under acidogenic conditions to achieve butyric acid for further ABE fermentation in solventogenesis stage. The main effects of initial pH (5 and 7), temperature (35 °C and 55 °C), and substrate concentration (40, 60, 80, and 100 g-VS/L) for hydrogen and butyric acid production were evaluated by using batch fermentation experiment. The major effects on hydrogen and butyric acid production are pH and temperature. The highest production of hydrogen and butyric acid was observed at pH 7 and temperature 35 °C. Using initial Chlorella sp. concentration of 80 g-VS/L or 100 g-VS/L at pH 7 and temperature 35 °C could produce hydrogen with an average yield of 22 mL-H2/g-VS along with high butyric acid production yield of 0.05 g/g-VS, suggesting that microalgae (Chlorella sp.) has potential to be converted directly to butyric acid by using acidogenesis under above optimum conditions.
This paper reports on the optimum conditions for simultaneous hydrogen and butyric acid production from microalgae (Chlorella sp.) using enriched anaerobic mixed cultures as inoculum. The fermentation was objectively carried out under acidogenic conditions to achieve butyric acid for further ABE fermentation in solventogenesis stage. The main effects of initial pH (5 and 7), temperature (35 °C and 55 °C), and substrate concentration (40, 60, 80, and 100 g-VS/L) for hydrogen and butyric acid production were evaluated by using batch fermentation experiment. The major effects on hydrogen and butyric acid production are pH and temperature. The highest production of hydrogen and butyric acid was observed at pH 7 and temperature 35 °C. Using initial Chlorella sp. concentration of 80 g-VS/L or 100 g-VS/L at pH 7 and temperature 35 °C could produce hydrogen with an average yield of 22 mL-H2/g-VS along with high butyric acid production yield of 0.05 g/g-VS, suggesting that microalgae (Chlorella sp.) has potential to be converted directly to butyric acid by using acidogenesis under above optimum conditions.
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Keywords
acidogenesis, butyric acid, Hydrogen, microalgae
Subject
Suggested Citation
Usmanbaha N, Jariyaboon R, Reungsang A, Kongjan P, Chu CY. Optimization of Batch Dark Fermentation of Chlorella sp. Using Mixed-Cultures for Simultaneous Hydrogen and Butyric Acid Production. (2023). LAPSE:2023.21160
Author Affiliations
Usmanbaha N: Energy Technology Program, Faculty of Engineering, Prince of Songkla University, Songkhla 90110, Thailand; Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000,
Jariyaboon R: Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand; Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani
Reungsang A: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand
Kongjan P: Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand; Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani
Chu CY: Master’s Program of Green Energy Science and Technology, Feng Chia University, Taichung 40724, Taiwan; Green Energy Development Center, Feng Chia University, Taichung 40724, Taiwan
Jariyaboon R: Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand; Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani
Reungsang A: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand
Kongjan P: Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand; Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani
Chu CY: Master’s Program of Green Energy Science and Technology, Feng Chia University, Taichung 40724, Taiwan; Green Energy Development Center, Feng Chia University, Taichung 40724, Taiwan
Journal Name
Energies
Volume
12
Issue
13
Article Number
E2529
Year
2019
Publication Date
2019-07-01
ISSN
1996-1073
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PII: en12132529, Publication Type: Journal Article
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LAPSE:2023.21160
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https://doi.org/10.3390/en12132529
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Mar 21, 2023
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